LOCUS       sequence-482120-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosomal purification of lysosome marker LAMP1-mScarlet-twin-strep.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LAMP1 mScarlet twin strep
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..138)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     388..407
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             695..715
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(722..727,728..751,752..787,788..811)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc
       61 cacgagttcg agatcgaggg cgagggcgag ggccgcccnt acgagggnca cccagaccgc
      121 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
      181 gttcatgtac ggctccaggg ccttcatcaa gcaccccgcc gacatccccg actactataa
      241 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
      301 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
      361 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
      421 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
      481 cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa
      541 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
      601 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
      661 cggcatggac gagctgtaca agggaggttc tggtgagaat ctctacttcc aatctggtgc
      721 tagcgcatgg agtcatcctc aattcgagaa aggtggaggt tctggcggtg gatcgggagg
      781 ttcagcgtgg agccacccgc agttcgaaaa ataaaagtaa agcggccgcg actctagatc
      841 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      901 cccctgaacc tgaaacataa aatgaatgcc aattgttgtt gttaacttgt tatgncgctt
      961 naana
//
