LOCUS       sequence-482139-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression and purification of rat wild-type Rab3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: twin strep HRV3C Flag Rab3a
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..152
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             171..194
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             195..218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             1108..1128
                     /estimated_length=21
     gap             1130..1143
                     /estimated_length=14
     gap             1148..1180
                     /estimated_length=33
     gap             1182..1196
                     /estimated_length=15
ORIGIN
        1 agnnnnnnng gttnngtgaa ccgtcagann ccgctagcgc tnaccggtcg ccaccatggg
       61 cagcgcatgg agtcatcctc aattncgaga aaggtggagg ttctggcggt ggatcgggag
      121 gttcagcgtg gagccacccg cagttcgaaa aatccggagg tgcttctggt ctggaagttc
      181 tgttccaggg acccgattac aaggatgacg acgataagtc cggactcaga tctcgagctc
      241 aagcttcgaa ttctgcagtc gacatggcct cagccacaga ctctcgatat gggcagaagg
      301 agtcctcaga ccagaacttc gactatatgt tcaagatcct gatcattggt aacagcagtg
      361 tgggcaaaac ctcattcctt ttccgctacg cagacgactc ttttactcca gcctttgtca
      421 gcactgtggg catagacttc aaggtcaaaa ccatctaccg caatgacaag aggatcaagc
      481 tgcagatctg ggacacagca ggacaagagc ggtaccgaac catcaccaca gcctactacc
      541 ggggcgccat gggcttcatt ctaatgtatg acatcaccaa tgaggagtcc ttcaatgcag
      601 tgcaggactg gtccactcag atcaaaactt actcatggga caatgcccag gtgctgctgg
      661 tggggaacaa gtgcgacatg gaggacgagc gagtggtgtc ctcagaacga ggccggcagc
      721 tggccgacca cctgggcttt gagttctttg aggccagcgc caaggacaac attaatgtca
      781 agcaganctt tgaacgtctg gtggacgtga tctgtgagaa gatgtcggan tccctagata
      841 ctgcagaccc tgcagtcacg ggtgccaagc agggcccaca gctcaccgac cagcnnnacc
      901 acctcatcng gantgcgcct gctgaggatc caccggatct aganactgat cataatcagc
      961 cntaccacat ttgtagaggt tttacttgct nnaaaanctc ccanacnccc ctganntgaa
     1021 acatnannga ngnnnntnnn tnnntntnnt gnagctnnan nnnnnnaann annncatcnn
     1081 nantncnnnn nnancntttt tnnnngcnnn nnnnnnnnnn nnnnnnnncn nnnnnnnnnn
     1141 nnncnnannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnnnnn nnnnnncnna
     1201 nnnnnnnnnc nnnnnannnn naa
//
