LOCUS       sequence-48215-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2359
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             92..157
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaatagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcaccat ggactacaaa gaccatgacg gtgattataa
      121 agatcatgac atcgattaca aggatgacga tgacaagatg gcccccaaga agaagaggaa
      181 ggtgggcatt caccgcgggg tacctatggt ggacttgagg acactcggtt attcgcaaca
      241 gcaacaggag aaaatcaagc ctaaggtcag gagcaccgtc gcgcaacacc acgaggcgct
      301 tgtggggcat ggcttcactc atgcgcatat tgtcgcgctt tcacagcacc ctgcggcgct
      361 tgggacggtg gctgtcaaat accaagatat gattgcggcc ctgcccgaag ccacgcacga
      421 ggcaattgta ggggtcggta aacagtggtc gggagcgcga gcacttgagg cgctgctgac
      481 tgtggcgggt gagcttaggg ggcctccgct ccagctcgac accgggcagc tgctgaagat
      541 cgcgaagaga gggggagtaa cagcggtaga ggcagtgcac gcctggcgca atgcgctcac
      601 cggggccccc ttgaacctga ccccagacca ggtagtcgca atcgcgtcaa acggaggggg
      661 aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc ctttgtcaag accacggcct
      721 tacaccggag caagtcgtgg ccattgcaag caatggggg
//
