LOCUS       sequence-482159-       10383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of Arhgef11-Cry2-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Krueger D, Spoelstra WK, Mastebroek DJ, Kok RNU, Wu S, Nikolaev M, 
            Bannier-Helaouet M, Gjorevski N, Lutolf M, van Es J, van Zon J, Tans
            SJ, Clevers H
  TITLE     Epithelial tension controls intestinal cell extrusion.
  JOURNAL   Science. 2025 Sep 4;389(6764):eadr8753. doi: 
            10.1126/science.adr8753. Epub 2025 Sep 4.
  PUBMED    40906841
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-09-4"; volume: "389(6764):eadr8753. doi"; pages: " 
            10.1126/science.adr8753. Epub 2025 Sep 4"
COMMENT     Sequence Label: p2T-TetON-Hygro-Arhgef11-Cry2-mCh
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    51..185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        323..690
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    367..385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    403..421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    439..457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    475..493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    511..529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    547..565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1885..3378
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      3397..3406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3403..4113
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3548..3566)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3816..3835
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4135..4269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     4439..4459
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(4469..4656,4657..4668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4725..4745)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4725..4743)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4764..4780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4764..4780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4777..4799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4788..4804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4812..4818,4819..4836,4837..4842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4857..4878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4995..5012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5166..5754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5246..5265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5925..6716,6717..6785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6548..6567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6786..6890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7053..7074)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7265..7284
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7428..7450
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7442..7459
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7443..7459
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7469..7487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(7518..7529,7530..7692)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      7717..7726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7734..8759)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8760..8816)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8826..8879)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(8880..9623)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(9697..9900)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9726..9746)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9901..10280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aagcttgctc gacattgatt attgactagt ctagtggatc caccgatgat gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      241 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc
      301 agatctgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa cgtatctaca
      361 gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag
      421 aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta
      481 tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca
      541 gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac cagggcgcct
      601 ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg tcttatacca
      661 actttccgta ccacttccta ccctcgtaaa gaattgttta aacgatatcg caccatgctg
      721 acccagagag aaattgatag gcaggaagtg attaacgagc tgtttgtgac cgaggcaagc
      781 catctgagaa ccctgagagt gctggacctg atcttctacc agaggatgaa gaaagagaac
      841 ctgatgcccc gggaggaact ggctagactg tttccaaacc tgcccgagct gatcgaaatt
      901 cacaattcct ggtgcgaagc aatgaagaaa ctgcgcgagg aaggacctat cattaaggag
      961 atttctgacc tgatgctggc taggttcgat ggcccagcac gcgaggaact gcagcaggtg
     1021 gccgctcagt tttgctcata ccagagcatc gccctggaac tgattaagac taaacagaga
     1081 aaggagtcta ggttccagct gtttatgcag gaggctgaaa gtcacccaca gtgtcggaga
     1141 ctgcagctgc gcgacctgat cattagcgaa atgcagcgac tgacaaagta tcccctgctg
     1201 ctggaaagca tcatcaagca cactgagggc gggacctccg agcatgaaaa gctgtgcagg
     1261 gcccgcgatc agtgtcggga gatcctgaag tacgtgaacg aggctgtcaa acagaccgaa
     1321 aatcgacatc ggctggaggg ctatcagaaa agactggacg ctacagcact ggaaagggcc
     1381 agtaaccccc tggcagccga gttcaagtca ctggatctga ccacacggaa aatgatccac
     1441 gaggggcctc tgacctggag aattagtaag gacaaaacac tggatctgca tgtgctgctg
     1501 ctggaagacc tgctggtcct gctgcagaag caggatgaga aactgctgct gaagtgccac
     1561 agcaaaactg cagtgggcag ctccgactcc aagcagacct tttctcctgt cctgaaactg
     1621 aatgccgtgc tgatccgatc agtcgcaaca gataagcggg ccttctttat catttgtact
     1681 agcaaactgg ggccccctca gatctacgaa ctggtggccc tgacatctag tgataagaac
     1741 acttggatgg agctgctgga ggaagccgtg cgcaatgcta cccgacatcc aggagagttc
     1801 tccgcagggg ggtcagcagg ggggtccgca ggggggtccg caggggggtc agcaggaggg
     1861 gctctcgagg ccaccatcgc caccatgaag atggacaaaa agactatagt ttggtttaga
     1921 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
     1981 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
     2041 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
     2101 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
     2161 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
     2221 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     2281 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     2341 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     2401 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     2461 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     2521 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     2581 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     2641 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     2701 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     2761 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     2821 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     2881 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     2941 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     3001 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     3061 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     3121 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     3181 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     3241 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     3301 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
     3361 atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     3421 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3481 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3541 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3601 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3661 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3721 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3781 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3841 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     3901 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3961 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4021 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4081 tccaccggcg gcatggacga gctgtacaag tagtctagtg gatccaccga tgatgatcca
     4141 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4201 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4261 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4321 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
     4381 gatcagatct tcgatggatc cagaccacct cccctgcgag cccgtcgacc tctagcacac
     4441 cctaactgac acacattcca cagaattcaa gtgatctcca aaaaataagt actttttgac
     4501 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     4561 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     4621 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     4681 ctaccttcag caagcccagc agatccgagc tccagctttt gttcccttta gtgagggtta
     4741 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4801 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4861 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4921 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4981 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5041 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5101 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5161 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5221 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5281 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5341 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5401 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5461 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5521 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5581 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5641 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5701 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5761 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5821 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5881 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5941 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6001 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6061 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6121 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6181 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6241 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6301 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6361 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6421 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6481 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6541 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6601 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6661 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6721 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6781 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6841 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6901 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     6961 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7021 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7081 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7141 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7201 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     7261 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgcg tcccattcgc cattcaggct
     7321 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7381 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7441 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     7501 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
     7561 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
     7621 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
     7681 taaaagcaag aaagatcgat ctcgaaggat ctggaggcca ccatggtgtc gatttattcc
     7741 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     7801 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     7861 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     7921 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     7981 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     8041 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     8101 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     8161 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     8221 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     8281 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc acgtagtgta ttgaccgatt
     8341 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     8401 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     8461 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     8521 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgatg aacataacga
     8581 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     8641 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     8701 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     8761 ggtccagggt tctcctccac gtctccagcc tgcttcagca ggctgaagtt agtagctccg
     8821 gatcctgggc caggattctc ctcgacgtca ccgcatgtta gcagacttcc tctgccctcc
     8881 ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat atcaaggtca
     8941 aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggtc
     9001 ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag ttccaggccg
     9061 aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg tttcagaagt
     9121 gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg atgctcctgt
     9181 tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta cagtgcgttc
     9241 tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt ttcgtactgt
     9301 ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga gaggagagca
     9361 cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc ttccaggggg
     9421 caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag cagggcccgc
     9481 ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg agcgagtttc
     9541 cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga gtttatgact
     9601 ttgctcttgt ccagtctaga catgctagcg gatctcatag tgagtcgtat taatttcgat
     9661 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
     9721 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     9781 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     9841 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     9901 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     9961 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
    10021 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
    10081 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
    10141 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
    10201 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
    10261 actagtcaat aatcaatgtc aacgcgtata tctttaccgt aagttatgta acgaaagcaa
    10321 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagcggcgc gccgaattct
    10381 acc
//
