LOCUS       sequence-48221-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK
  TITLE     FLASH assembly of TALENs for high-throughput genome editing.
  JOURNAL   Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.
  PUBMED    22484455
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170."
COMMENT     Sequence Label: TAL2222
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..145
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      121 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca
      181 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      241 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      301 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      361 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      421 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      481 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      541 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt
      601 gaacctgacc ccagaccagg tagtcgcaat cgcgtcacat gacgggggaa agcaagccct
      661 ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac cacggcctta caccggagca
      721 agtcgtggcc attgcaagca atgggggtgg caaacaggct cttgagacgg ttcagagact
      781 tctcccagtt ctctgtcaag cccacgggct gactcccgat caagttgtag cgattgcgtc
      841 gcatgacgga gggaaacaag cattggagac tgtc
//
