LOCUS       sequence-482212-       10475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Myc-tagged catalytic dead TRIP12 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Kassel S, Yuan K, Bunnag N, Neitzel LR, Lu W, Schwarzkopf A, Maines 
            B, Loberg MA, Xu G, Adams A, McCray AD, Cho A, Rockouski M, Orton G,
            Goldsmith L, Aronno MMA, Spencer ZT, Khan OM, Ye F, Williams C, 
            Lebensohn AM, Rohatgi R, Wang X, Weiss VL, Hong CC, Kettenbach AN, 
            Robbins DJ, Ahmed Y, Lee E
  TITLE     The TRIP12 E3 ligase induces SWI/SNF component BRG1-beta-catenin 
            interaction to promote Wnt signaling.
  JOURNAL   Nat Commun. 2025 Jun 5;16(1):5248. doi: 10.1038/s41467-025-60535-5.
  PUBMED    40473626
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-06-5"; volume: "16(1):5248. doi"; pages: " 
            10.1038/s41467-025-60535-5"
COMMENT     Sequence Label: pCS2-Myc-TRIP12-CD
FEATURES             Location/Qualifiers
     source          1..10475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6527..6661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6551..6570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6605..6624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6774..6794)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6774..6792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6813..6829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6813..6829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6826..6848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6837..6853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6861..6867,6868..6885,6886..6891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6906..6927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7044..7061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7215..7803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7295..7314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7974..8765,8766..8834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8597..8616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8835..8939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8965..9420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9102..9123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9314..9333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9491..10475
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaggc
      361 cggcccatgt ccaaccggcc taataacaat ccaggggggt cactgcgacg ttcacagagg
      421 aacactgccg gggcccaacc acaagacgac tcaataggag gaaggtcaca tttagggcag
      481 gcaaaacata agggatatag ccctcctgag agtagaaaat ctaattctaa ggcacccaaa
      541 gtgcagtcta atactacttc tgaactgtca aggggacacc tttctaaaag aagctgcagt
      601 tcatcatctg ctgtgatagt tccacaacca gaggatccag acagagccaa tacttcagaa
      661 agacaaaaaa cggggcaggt gcctaagaaa gacaattctc gaggagtgaa gcgcagtgct
      721 agtccagact acaacaggac caattctcct agctctgcaa aaaaaccaaa agcacttcag
      781 catactgaat ctccctcaga aacaaataag ccacatagta agtcaaagaa gagacattta
      841 gaccaggagc aacaactgaa atctgcacaa tcaccatcaa caagcaaggc tcataccagg
      901 aagagtgggg ccactggcgg ttcacggagt cagaaaagaa aaaggacaga gagttcttgt
      961 gtaaagagtg gctccgggtc tgaatcaact ggtgcagaag agagatctgc gaaacctacc
     1021 aagctggctt caaaatcagc cacctcagcc aaagctgggt gtagcaccat cactgattct
     1081 tcttctgctg cctctacttc ctcctcgtct tctgctgtag cctcggcctc ctccactgta
     1141 ccaccaggtg ccagagtgaa acaaggaaaa gatcagaaca aggccaggcg ttcccgttca
     1201 gcgtccagtc ccagccccag aagaagtagc agggaaaagg aacagagtaa aactggtggc
     1261 tcttcaaaat ttgattgggc tgctcgtttc agccctaaag ttagccttcc taaaacaaaa
     1321 ctgtctcttc cagggtcttc taagtcagag acatcaaaac ctggaccttc tggattacag
     1381 gccaaattag caagtttaag aaaatctacg aagaaacgca gtgagtctcc acctgctgag
     1441 ctccccagtt tgaggcggag cacacgccaa aagaccacgg gctcctgtgc tagtaccagt
     1501 cggcgaggct ctggcctggg caaaagagga gcagctgaag ctcgtcgaca ggagaaaatg
     1561 gcagaccctg aaagcaacca ggaggcagta aattcttcag ctgctcggac agatgaagct
     1621 ccccaaggag ctgcagcctc tagttctgtt gcaggggctg ttggcatgac cacctctggg
     1681 gagagtgaat cagatgattc cgagatggga cgtttgcaag ctttgttaga ggcaaggggt
     1741 cttccccctc acctatttgg tcctcttggt cctcggatgt cacagctttt ccatagaaca
     1801 attggaagtg gagctagttc taaggcccag cagctactac aaggattgca agccagtgat
     1861 gaaagtcaac agcttcaggc agttattgag atgtgtcagt tactggtcat gggaaatgag
     1921 gagacactgg gagggtttcc tgtcaagagt gttgttccag ctttgattac gttacttcag
     1981 atggagcaca attttgatat tatgaaccat gcttgtcgag ccttaacata catgatggaa
     2041 gcacttcctc gatcttctgc tgttgtagta gatgctattc ctgtcttttt agaaaagctg
     2101 caagttattc agtgtattga tgtggcagag caggccttga ctgccttgga gatgttgtca
     2161 cggagacata gtaaagccat tctacaggcg ggtggtttgg cagactgctt gctgtaccta
     2221 gaattcttca gcataaatgc ccaaagaaat gcattagcaa ttgcagctaa ttgctgccag
     2281 agtatcacgc cagatgaatt tcattttgtg gcagattcac tcccattgct aacccaaagg
     2341 ctaacacatc aggataaaaa gtcagtagaa agcacttgcc tttgttttgc acgcctagtg
     2401 gacaacttcc agcatgagga gaatttactc cagcaggttg cttccaaaga tctgcttaca
     2461 aatgttcaac agctgttggt agtgactcca cccattttaa gttctgggat gtttataatg
     2521 gtggttcgca tgttttctct gatgtgttcc aactgtccaa ctttagctgt tcaacttatg
     2581 aaacaaaaca ttgcagaaac gcttcacttt ctcctgtgtg gtgcctccaa tggaagttgt
     2641 caggaacaga ttgatcttgt tccacgaagc cctcaagagt tgtatgaact gacatctctg
     2701 atttgtgaac ttatgccatg tttaccaaaa gaaggcattt ttgcagttga taccatgttg
     2761 aagaagggaa atgcacagaa cacagatggt gcgatatggc agtggcgtga tgatcggggc
     2821 ctctggcatc catataacag gattgacagc cggatcattg agcaaatcaa tgaggacacg
     2881 ggaacagcac gtgccattca gagaaaacct aacccgttag ccaatagtaa cactagtgga
     2941 tattcagagt caaagaagga tgatgctcga gcacagctta tgaaagagga tccggaactg
     3001 gctaagtctt ttattaagac gttatttggt gttctttatg aagtgtatag ttcctcagca
     3061 ggacctgcgg tcagacataa gtgccttaga gcaattctta ggataattta ttttgcggat
     3121 gctgaacttc tgaaggatgt tctgaaaaat catgctgttt caagtcacat tgcttccatg
     3181 ctgtcaagcc aagacctgaa gatagtagtg ggagcacttc agatggcaga aattttaatg
     3241 cagaagttac ctgatatttt tagtgtttac ttcagaagag aaggtgtaat gcatcaagta
     3301 aaacacttag cagaatcaga gtctttgttg acaagtccac caaaggcatg tacgaatgga
     3361 tcgggatcca tgggatccac aacttcagtc agcagtggga cagccacagc tgccactcat
     3421 gctgcagctg acttgggatc acccagcttg cagcacagca gggatgattc tttagatctc
     3481 agccctcaag gtcgattaag tgatgttcta aagagaaaac gactgccaaa acgagggcca
     3541 agaaggccaa agtactcacc tccaagagat gatgacaaag tagacaatca agctaaaagc
     3601 cccaccacta ctcagtcacc taaatcttct ttcctggcaa gcttgaatcc aaaaacatgg
     3661 ggaaggttaa gtacacagtc caacagcaac aacattgagc cagcacggac tgcgggaggt
     3721 agtggccttg ccagggctgc ctcaaaggat accatctcca ataatagaga aaaaattaaa
     3781 ggttggatta aggagcaggc acataaattt gtagaacgtt atttcagttc tgagaatatg
     3841 gatggaagca accctgcatt gaatgtcctt cagagacttt gtgctgcaac cgaacaactc
     3901 aacctccagg tggatggtgg agctgagtgc cttgtagaaa tccgtagcat agtctcagag
     3961 tcagatgttt catcatttga aatccaacat agtggatttg tgaagcagct gttgctttat
     4021 ttgacatcta aaagtgaaaa ggatgctgtg agcagagaga tcagattaaa gcgatttctt
     4081 catgtatttt tttcttctcc acttcctgga gaagagccca ttggaagagt ggaaccagtg
     4141 ggtaatgcac ctttgttggc attagttcac aagatgaaca actgcctcag ccagatggaa
     4201 caatttccag tcaaagtaca tgatttccct agtggaaatg ggacaggagg cagcttttct
     4261 ctcaacagag gatcacaggc tttaaaattt ttcaacacac atcaattaaa atgccagtta
     4321 caaaggcatc cagactgtgc aaatgtgaag cagtggaagg gtggacctgt caagattgac
     4381 cctctggctt tggtacaagc catcgagaga taccttgtag ttagagggta tggaagagta
     4441 agagaagatg atgaagacag cgatgacgat ggatcagatg aggaaataga tgagtctctg
     4501 gctgctcagt tcctaaattc aggaaatgta agacacaggc tgcagtttta tattggagaa
     4561 catttgctgc cgtataacat gactgtgtat caggcagtac ggcagtttag tatacaggct
     4621 gaagatgaaa gagaatccac agatgatgag agcaatcctc taggcagagc tggtatttgg
     4681 acaaagactc atacaatatg gtataaacct gtgagagagg atgaagaaag taataaagat
     4741 tgtgttggtg gtaaaagagg aagagcccaa acagctccaa cgaaaacttc ccctagaaat
     4801 gcaaaaaagc atgatgagtt atggcacgat ggagtgtgcc catcagtatc aaatccttta
     4861 gaagtttacc tcattcccac accacctgaa aatataacat ttgaagaccc gtcattagat
     4921 gtgatccttc ttttaagagt tttacatgct atcagtcgat actggtatta cttgtatgat
     4981 aatgcaatgt gcaaggaaat tattccaact agtgaattta ttaacagtaa gttaacagca
     5041 aaagcaaata ggcaacttca agatccttta gtaatcatga caggaaacat cccaacatgg
     5101 cttactgagc taggaaaaac ctgcccattt ttctttcctt ttgatacccg gcaaatgctt
     5161 ttttatgtaa ctgcatttga tcgggaccga gcaatgcaaa gattacttga taccaaccca
     5221 gaaatcaacc agtctgattc tcaagatagc agagttgcac ctagattgga tagaaaaaaa
     5281 cgtactgtga accgagagga gctgctgaaa caggcggagt ctgtgatgca ggacctcggc
     5341 agctcacggg ccatgttaga aatccagtat gaaaatgagg ttggtacagg tcttgggcct
     5401 acactggagt tttatgcgct tgtatctcag gaactacaga gagctgactt gggtctttgg
     5461 agaggtgaag aagtaactct tagcaatcca aaagggagcc aagaagggac caagtatatt
     5521 caaaacctcc agggcctgtt tgcgcttccc tttggtagga cagcaaagcc agctcatatc
     5581 gcaaaggtta agatgaagtt tcgcttctta ggaaaattaa tggccaaggc tatcatggat
     5641 ttcagattgg tggaccttcc ccttggctta cccttttata aatggatgct acggcaagaa
     5701 acttcactga catcacacga tttgtttgac atcgacccag ttgtagccag atcagtttat
     5761 cacctagaag acattgtcag acagaagaaa agacttgaac aagataaatc ccagaccaaa
     5821 gagagtctac agtatgcatt agaaaccttg actatgaatg gctgctcagt tgaagatcta
     5881 ggactggatt tcactctgcc agggtttccc aatatcgaac tgaagaaagg agggaaggat
     5941 ataccagtca ctatccacaa tttagaggag tatctaagac tggttatatt ctgggcacta
     6001 aatgaaggcg tttctaggca atttgattcg ttcagagatg gatttgaatc agtcttccca
     6061 ctcagtcatc ttcagtactt ctacccggag gaactggatc agctcctttg tggcagtaaa
     6121 gcagacactt gggatgcaaa gacactgatg gaatgctgta ggcctgatca tggttatact
     6181 catgacagtc gggctgtgaa gtttttgttt gagattctca gtagttttga taatgagcag
     6241 cagaggttat ttctccagtt tgtgactggt agcccaagat tgcctgttgg aggattccgg
     6301 agtttgaatc cacctttgac aattgtccga aagacgtttg aatcaacaga aaacccagat
     6361 gacttcttgc cctctgtaat gactgctgtg aactatctta agttgccgga ctattcaagc
     6421 attgagataa tgcgtgaaaa actgttgata gcagcaagag aagggcagca gtcgttccat
     6481 ctttcctaag gcgcgcctct agaactatag tgagtcgtat tacgtagatc cagacatgat
     6541 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6601 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6661 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6721 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag
     6781 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6841 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
     6901 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6961 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7021 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7081 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7141 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7621 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7981 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8041 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8101 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8161 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8221 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8281 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8341 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8581 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8941 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     9001 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     9061 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     9121 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     9181 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     9241 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     9301 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     9361 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     9421 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9481 tattacgcca gtcgaccgcc aattcaatat ggcgtatatg gactcatgcc aattcaatat
     9541 ggtggatctg gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat
     9601 ggtggatctg gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat
     9661 ggcggacctg gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag
     9721 gaggggtctt ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc
     9781 ggccatattg gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc
     9841 aatatggcta ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat
     9901 atatgggttt tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat
     9961 atggggcttc ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata
    10021 tggtctttcc tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat
    10081 tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca
    10141 cccaccattg acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct
    10201 attgacgtca atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa
    10261 cttggcaagt acattactat tggaagtacg ccagggtaca ttggcagtac tcccattgac
    10321 gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg
    10381 gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat
    10441 gggaggtcta tataagcaat gctcgtttag ggaac
//
