LOCUS       sequence-48234-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, 
            Tsien RY
  TITLE     Ca2+ indicators based on computationally redesigned 
            calmodulin-peptide pairs.
  JOURNAL   Chem Biol. 2006 May;13(5):521-30.
  PUBMED    16720273
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2006-05"; volume: "13(5)"; pages: "521-30"
COMMENT     Sequence Label: pcDNA-lynD3cpV
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(158..160,161..163,164..676)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(203..224)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(464..483)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttgcggccgc caccatgggc tgcatcaaga gcaagcgcaa
      121 ggacaacctg aacgacgacg gcgtggacat gaagaccatg gtgagcaagg gcgaggagct
      181 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt
      241 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      301 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
      361 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      421 catgcccgaa ggctacgtcc aggagcgtac catcttcttc aaggacgacg gcaactacaa
      481 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      541 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
      601 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat
      661 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      721 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      781 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
//
