LOCUS       sequence-482344-        9890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Csp1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Csp1
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1251..1259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(2614..2694)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             3594..4559
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     terminator      4584..4670
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4708..5102
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5209..6180
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6691..7530
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7517..8368
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8379..8487
                     /note="incP origin of transfer"
     CDS             complement(8654..9469)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9461..9480
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(9601..9695)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ctgaccccaa ccgccctgcg gctgaccacc accgatatta ccacctgccg gagcgccacc
       61 accctgacga ccacccagca tctgcatggt gccgccaacg ttcaccacga cttctgtggt
      121 gtagcgatcc tgaccggatt gatcggtcca tttacgggta cgcagctgac cttcgatata
      181 aacctgagaa cctttacgca gatattcgct ggccacttct gccagtttgc cgaacagcac
      241 aacgcggtgc cattcagtct gctctttcat ctcgccggtc gctttatcac gccaggattc
      301 ggaagtagcc agcgtaatgt tggcaactgc gccaccattt ggcatgtagc gtacttccgg
      361 gtcctgaccc agattaccaa cgagaataac cttgtttacg cctctgctgg ccatgtgtat
      421 tctcctggat ccttaaaaca cctcgccctc gtcaacaacc tcatactccg cttccacagc
      481 tggcgcagcc atttcttcct ccatccaagc gcgagcacgg cgagcttgct gctcggtcat
      541 agagtgcatg ccttcagcgc ccaccgcacc acaaacagca gtcataccct cagccggagt
      601 gatgccgaag tgttcgcaat atggcttgaa gagttcgcgg attggctgga gatccactgg
      661 ttgctccgcc tgagcgcgca cctgttctgg ctgttgcgct tgatccattt cctccgcagc
      721 gtacatgccc tgaaaagtgt ccgggaacgc gaggcgccaa gcagcagcct tcgcgcattt
      781 ttcgatcata acacccggca ttttcgccca attagatttg ccggtggaat agtcgtcgag
      841 cgccacctca gcatacgcag tctcgcggcc atccttaaag cgaacttccg cccaaccacc
      901 gatgagctcc tcacccgcct ttttgtagca cgcagcacca cgtttatgca caacatcacc
      961 gtcacgcaga acaaccacac cagatttgat accatcatag ttagcatcac gacacgcgcg
     1021 acggttaaaa acttgatagg aagtaatcat agaagctgga gcaccgccgt atttcaccag
     1081 gtaagcgtct ttcacaaacg gattcagacg ttgagtctga cagagttgca gaaagaggcc
     1141 cacctctttt tcagtagcgt tctcacagat ataacggcga acatcgtctg gagtaacttg
     1201 aaccgccagg ccagagtcgt cagtgaactt cacgatttgg ttcattccaa acctccttaa
     1261 agttaaacaa aattatttcg atcgagtgcg gccgcggatc atgcaggtgg caagccggac
     1321 ggcacaatac accgataccg taagctgcgg gtgaagagct attcgtaagg gacaggttga
     1381 agctacaacg gctcacactc gctcttcact tacagccgaa ggcggtgtgc acacggaagt
     1441 gtgcggatgt actttggagc ggattacggg tgcagtattc tgcatgccca ctgtttctaa
     1501 ctcccggcgg ggactccccg tcaggccgga aaatattttc ccggatatgc tgccggccgg
     1561 cttatcacct gctctagcct catatgaacg gtattatttt acaaaccgct ctttccatat
     1621 cctgcagtat tccgcaaatt ccctgtcctc cggattcacc tgcagcacaa actgaattct
     1681 tccaaggaca gaacatatat acctctgctc ctccaccgca ttcccggtct cctccctcag
     1741 cctttcaaga tgccctgtta cgccatattt catgcagtaa tagatttcct gccgcagttt
     1801 tttacggtaa tccctggaga cctggggctt ttcattgacc acaattcccg tcacactctg
     1861 gcgtacgccg tccttcagga gctttgtttt cttttcattt aaggaaaatc ccatcgcctc
     1921 cagatagctt tttaccttat gataaacagc ggcgggatca aaatctccgg aaaaggtcat
     1981 gtcatcgcag tatctggtgt atacgatctg tctcgcttca caccagcgcc tcatggattc
     2041 atcaaagggg cgcatcacca gattggagat ggccggggaa gccggagagc cctggggcag
     2101 gcgttcccgg cagcagcaca ggtttgccag aagcgcggcg gcggccggag gaaacagcgt
     2161 atccgggaag gcggcgctga taaccctggg aaagagaata ctgtcaaaaa aatggctgat
     2221 atccagcttg agcacctgct ttctcccggt atgcggcccg gcattggcca ggatgtccgt
     2281 gcctttccga taggccctgg cgcagtccga tacgggcctt tgctccagca catggtgcag
     2341 gatattgcgc tgcacggcct tcagtaggct gcagggaacc ataagcgtcc ttgtcccgcc
     2401 atcctttttt gtcagaatct ccttatggta attctccgca ccacggttgc tgagcatata
     2461 aagacaggca agctgctttt ccctgctcat accggcgctg tgagggacaa gatccagcga
     2521 aaggataaag tcctggcatt gttctttggt acagtatttc caaagttgcg aatttgacat
     2581 atgtatatct ccttcgaatt cggatcctag aggttgcata aagcctaagg ggtaggcctt
     2641 actagagata gccatttttt gcactcctgt atccgcttct tgcaaggctg gacttatccc
     2701 tatcaaaccg gacactgcac tttatgctgg ttatgctggc tgcatggggc attagttggg
     2761 gagccagatt tgtcatggag caggccgttc tgctttatgg atcaggaaaa aactatttgt
     2821 tcttcagtca tggtactgtt ctgatgtacc tgctgtgtgt tttcctggta taccgccgtt
     2881 ggatagctcc gctaccggtc gttggtcaga tcaagctaat tcccatgcat ggtgaaaacg
     2941 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3001 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     3061 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     3121 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     3181 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgtaa ttacggatga
     3241 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     3301 tttacggtct ttaaaaaggc cgtaatatcc agcagatccc ctttatccgc caattcgtcg
     3361 ccccatgccc cgacagcacg aacttctggt gttctcgctt cttaaaaaga acgtcttcgt
     3421 tctgcttggc gttatttttg cttggaaaag tggtcactga ttgcaaaaag gatggcgcaa
     3481 cgtggcaatg ggggtaaccc gtatacgcat cacgtcgaga tgcattttca tcgacttggc
     3541 gcctttctac atcacaccaa gcagcccaca ttaaaataag agaaccgtga actatggatt
     3601 tttgcttatt gaacgagaaa agtcagatct tcgtccacgc cgagccctat gcagtctccg
     3661 attatgttaa ccagtatgtc ggtacgcact ctattcgcct gcccaagggc gggcgcccgg
     3721 caggcaggct gcaccacaga atcttcggat gcctcgatct gtgtcgaatc agctacggcg
     3781 gtagcgtgag ggtaatctcg cctggattag agacctgtta tcatctgcaa ataatactca
     3841 aaggccattg cctgtggcgt ggccatggcc aggagcacta ttttgcgccg ggcgaactat
     3901 tgctgctcaa tccggatgac caagccgacc tgacctattc agaagattgc gagaaattta
     3961 tcgttaaatt gccctcagtg gtccttgatc gggcgtgcag tgacaacaat tggcacaagc
     4021 cgagggaggg tatccgtttc gccgcgcgac acaatctcca gcaactcgat ggctttatca
     4081 atctactcgg gttagtttgt gacgaagcgg aacatacaaa gtcgatgcct cgggtccaag
     4141 agcactatgc ggggatcatc gcttccaagc tgctcgaaat gctgggcagc aatgtcagcc
     4201 gtgaaatttt cagcaaaggt aacccgtctt tcgagcgagt cgttcaattc attgaggaga
     4261 atctcaaacg gaatatcagc cttgagcggt tagcggagct ggcgatgatg agtccacgct
     4321 cgctctacaa tttgttcgag aagcacgccg gcaccacgcc gaagaactac atccgcaacc
     4381 gcaagctcga aagcatccgc gcctgcttga acgatcccag tgccaatgtg cgtagtataa
     4441 ctgagatagc cctagactac ggcttcttac atttgggacg cttcgctgaa aactatagga
     4501 gcgcgttcgg cgagttgcct tccgacaccc tgcgtcaatg caaaaaggaa gtggcttgat
     4561 tacgaacgtt taattaaagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4621 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     4681 agacagctgg gcgcgcccta actgtcacga acccctgcaa taactgtcac gcccccctgc
     4741 aataactgtc acgaacccct gcaataactg tcacgccccc aaacctgcaa acccagcagg
     4801 ggcgggggct ggcggggtgt tggaaaaatc catccatgat tatctaagaa taatccacta
     4861 ggcgcggtta tcagcgccct tgtggggcgc tgctgccctt gcccaatatg cccggccaga
     4921 ggccggatag ctggtctatt cgctgcgcta ggctacacac cgccccaccg ctgcgcggca
     4981 gggggaaagg cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga
     5041 gcgcttttag ccgctttagc ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag
     5101 ccccgcaggg cctgtctcgg tcgatcattc agcccggctc atccttcgga tctagctaca
     5161 ctttatgctt ccggctcgta taatgtgtgg gaatcgcttg gtggcccgat gaagaacgac
     5221 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc
     5281 ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg
     5341 ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg
     5401 cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg
     5461 gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac
     5521 tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc
     5581 cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc
     5641 cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg
     5701 tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag
     5761 caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc
     5821 agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac
     5881 cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt
     5941 atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg
     6001 gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag
     6061 cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca
     6121 cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag
     6181 tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt
     6241 atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat
     6301 tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc
     6361 gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag
     6421 cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac
     6481 cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa
     6541 ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg
     6601 cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta
     6661 accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg
     6721 gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt
     6781 acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg
     6841 gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg
     6901 gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt
     6961 ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg
     7021 ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc
     7081 gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa
     7141 aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc
     7201 gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc
     7261 gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc
     7321 tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc
     7381 cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg
     7441 tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag
     7501 agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca
     7561 cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa
     7621 gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg
     7681 cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg
     7741 gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct
     7801 gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca aaggtagcta
     7861 tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat
     7921 acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg
     7981 gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt
     8041 ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag
     8101 catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg
     8161 tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt
     8221 ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc
     8281 gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga
     8341 catcacccgg cccaaggcgg caggctgagg ccggcctacg gccagcctcg cagagcagga
     8401 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
     8461 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
     8521 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
     8581 atcgctataa tgaccccgaa gcagggttat gcagcggaaa aggacaacgc gcggaccgcg
     8641 gtccaattaa ttattagaaa aattcatcca gcatcagatg aaattgcagt ttgttcatat
     8701 ccggattatc aatgccatat ttctgaaaca gacgtttttg caggctcggg ctaaattcgc
     8761 ccaggcagtt ccacagaatg gccagatcct gataacgatc cgcaatgccc acacggccca
     8821 catcaatgca gccaatcagt ttgccttcat cgaaaatcag gttatccagg ctaaaatcgc
     8881 cgtgggtcac cacgctatcc gggctaaacg gcagcagttt atgcatttct ttccacacct
     8941 gttccaccgg ccagccgtta cgttcatcat caaaatcgct cgcatccacc aggccgttgt
     9001 tcatacggct ctgcgcctgg gccagacgaa acacacgatc gctgttaaac gggcagttgc
     9061 acaccggaat gctatgcaga cgacgcagaa acacggccag cgcatccaca atgttttcgc
     9121 cgctatccgg atattcttcc agcacctgaa acgcggtttt gcccggaatc gcggtggtca
     9181 gcagccacgc atcatccggg gtgcgaataa aatgtttaat ggtcggcagc ggcataaatt
     9241 cggtcagcca gttcagacgc accatttcat cggtcacatc gttcgccacg ctgcctttgc
     9301 catgtttcag aaacagttcc ggcgcatccg gtttgccata cagacgataa atggtcgcgc
     9361 cgctctgacc cacgttatca cgcgcccatt tatagccata cagatccgca tccatgttgc
     9421 tgttcagacg cggacggcta cagctcgttt cacgctgaat atggctcata acaccccttg
     9481 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     9541 caatgtaaca tcagagattt tgagacacaa atttaaatcg taattattgg ggacccctgg
     9601 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     9661 ttctgaggtc attactggat ctatcaacag gagtccaaga ctagtcgcca gggttttccc
     9721 agtcacgacg cggccgcaag cttgcatgcc tgcaggtcga ctctagatca gaacggaatg
     9781 tcatcatcaa agtccatcgg cggctcgtta gacggcgctg ccggagcgga ctgctgcggg
     9841 cgagactgcg cgccgccgct gaactgattg ccaccctgcg gctgctgagg
//
