LOCUS       sequence-482346-        9844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Abe1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Abe1
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             469..1434
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     terminator      1459..1545
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1583..1977
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             2084..3055
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3566..4405
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4392..5243
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            5254..5362
                     /note="incP origin of transfer"
     CDS             complement(5529..6344)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6336..6355
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(6476..6570)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             8016..8024
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(9333..9413)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
       61 actatcccat atcaccagct caccgtcttt cattgccata cgtaattacg gatgagcatt
      121 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
      181 ggtctttaaa aaggccgtaa tatccagcag atccccttta tccgccaatt cgtcgcccca
      241 tgccccgaca gcacgaactt ctggtgttct cgcttcttaa aaagaacgtc ttcgttctgc
      301 ttggcgttat ttttgcttgg aaaagtggtc actgattgca aaaaggatgg cgcaacgtgg
      361 caatgggggt aacccgtata cgcatcacgt cgagatgcat tttcatcgac ttggcgcctt
      421 tctacatcac accaagcagc ccacattaaa ataagagaac cgtgaactat ggatttttgc
      481 ttattgaacg agaaaagtca gatcttcgtc cacgccgagc cctatgcagt ctccgattat
      541 gttaaccagt atgtcggtac gcactctatt cgcctgccca agggcgggcg cccggcaggc
      601 aggctgcacc acagaatctt cggatgcctc gatctgtgtc gaatcagcta cggcggtagc
      661 gtgagggtaa tctcgcctgg attagagacc tgttatcatc tgcaaataat actcaaaggc
      721 cattgcctgt ggcgtggcca tggccaggag cactattttg cgccgggcga actattgctg
      781 ctcaatccgg atgaccaagc cgacctgacc tattcagaag attgcgagaa atttatcgtt
      841 aaattgccct cagtggtcct tgatcgggcg tgcagtgaca acaattggca caagccgagg
      901 gagggtatcc gtttcgccgc gcgacacaat ctccagcaac tcgatggctt tatcaatcta
      961 ctcgggttag tttgtgacga agcggaacat acaaagtcga tgcctcgggt ccaagagcac
     1021 tatgcgggga tcatcgcttc caagctgctc gaaatgctgg gcagcaatgt cagccgtgaa
     1081 attttcagca aaggtaaccc gtctttcgag cgagtcgttc aattcattga ggagaatctc
     1141 aaacggaata tcagccttga gcggttagcg gagctggcga tgatgagtcc acgctcgctc
     1201 tacaatttgt tcgagaagca cgccggcacc acgccgaaga actacatccg caaccgcaag
     1261 ctcgaaagca tccgcgcctg cttgaacgat cccagtgcca atgtgcgtag tataactgag
     1321 atagccctag actacggctt cttacatttg ggacgcttcg ctgaaaacta taggagcgcg
     1381 ttcggcgagt tgccttccga caccctgcgt caatgcaaaa aggaagtggc ttgattacga
     1441 acgtttaatt aaaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagaca
     1561 gctgggcgcg ccctaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa
     1621 ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg
     1681 gggctggcgg ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc
     1741 ggttatcagc gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg
     1801 gatagctggt ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg
     1861 aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct
     1921 tttagccgct ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg
     1981 cagggcctgt ctcggtcgat cattcagccc ggctcatcct tcggatctag ctacacttta
     2041 tgcttccggc tcgtataatg tgtgggaatc gcttggtggc ccgatgaaga acgacaggac
     2101 tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt
     2161 cagggacgcc accaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca
     2221 gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc
     2281 cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga
     2341 catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca
     2401 ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac
     2461 gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt
     2521 ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt
     2581 gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc
     2641 tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct
     2701 cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag
     2761 cgagatggcc gggctggtca agcgcttcgg tgatgacctc agcaagtgcg actttatcgc
     2821 cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga
     2881 ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac
     2941 cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc
     3001 accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt
     3061 gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga
     3121 atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt
     3181 gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga
     3241 tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt
     3301 cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg
     3361 acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca
     3421 aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt
     3481 gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac
     3541 caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc
     3601 gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt
     3661 cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc
     3721 acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat
     3781 ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc
     3841 gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat
     3901 cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga
     3961 gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc
     4021 cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg
     4081 ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca
     4141 gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct
     4201 gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt
     4261 cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt
     4321 caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa
     4381 gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc
     4441 cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca
     4501 gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg
     4561 agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta
     4621 atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc
     4681 tggaacccaa gtgggaggcc gtcaccgctg atgccatggt ggtcaaaggt agctatcggg
     4741 cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg
     4801 actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg
     4861 ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc
     4921 tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg
     4981 acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct
     5041 ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc
     5101 cgtcagaggc cagtggttcg accatgcgca agcgccgcca gcgggtgcgc gaggcgttgc
     5161 cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca
     5221 cccggcccaa ggcggcaggc tgaggccggc ctacggccag cctcgcagag caggattccc
     5281 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     5341 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
     5401 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
     5461 tataatgacc ccgaagcagg gttatgcagc ggaaaaggac aacgcgcgga ccgcggtcca
     5521 attaattatt agaaaaattc atccagcatc agatgaaatt gcagtttgtt catatccgga
     5581 ttatcaatgc catatttctg aaacagacgt ttttgcaggc tcgggctaaa ttcgcccagg
     5641 cagttccaca gaatggccag atcctgataa cgatccgcaa tgcccacacg gcccacatca
     5701 atgcagccaa tcagtttgcc ttcatcgaaa atcaggttat ccaggctaaa atcgccgtgg
     5761 gtcaccacgc tatccgggct aaacggcagc agtttatgca tttctttcca cacctgttcc
     5821 accggccagc cgttacgttc atcatcaaaa tcgctcgcat ccaccaggcc gttgttcata
     5881 cggctctgcg cctgggccag acgaaacaca cgatcgctgt taaacgggca gttgcacacc
     5941 ggaatgctat gcagacgacg cagaaacacg gccagcgcat ccacaatgtt ttcgccgcta
     6001 tccggatatt cttccagcac ctgaaacgcg gttttgcccg gaatcgcggt ggtcagcagc
     6061 cacgcatcat ccggggtgcg aataaaatgt ttaatggtcg gcagcggcat aaattcggtc
     6121 agccagttca gacgcaccat ttcatcggtc acatcgttcg ccacgctgcc tttgccatgt
     6181 ttcagaaaca gttccggcgc atccggtttg ccatacagac gataaatggt cgcgccgctc
     6241 tgacccacgt tatcacgcgc ccatttatag ccatacagat ccgcatccat gttgctgttc
     6301 agacgcggac ggctacagct cgtttcacgc tgaatatggc tcataacacc ccttgtatta
     6361 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     6421 taacatcaga gattttgaga cacaaattta aatcgtaatt attggggacc cctggattct
     6481 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     6541 aggtcattac tggatctatc aacaggagtc caagactagt cgccagggtt ttcccagtca
     6601 cgacgcggcc gcaagcttgc atgcctgcag gtcgactcta gatcagaacg gaatgtcatc
     6661 atcaaagtcc atcggcggct cgttagacgg cgctgccgga gcggactgct gcgggcgaga
     6721 ctgcgcgccg ccgctgaact gattgccacc ctgcggctgc tgaggctgac cccaaccgcc
     6781 ctgcggctga ccaccaccga tattaccacc tgccggagcg ccaccaccct gacgaccacc
     6841 cagcatctgc atggtgccgc caacgttcac cacgacttct gtggtgtagc gatcctgacc
     6901 ggattgatcg gtccatttac gggtacgcag ctgaccttcg atataaacct gagaaccttt
     6961 acgcagatat tcgctggcca cttctgccag tttgccgaac agcacaacgc ggtgccattc
     7021 agtctgctct ttcatctcgc cggtcgcttt atcacgccag gattcggaag tagccagcgt
     7081 aatgttggca actgcgccac catttggcat gtagcgtact tccgggtcct gacccagatt
     7141 accaacgaga ataaccttgt ttacgcctct gctggccatg tgtattctcc tggatcctta
     7201 aaacacctcg ccctcgtcaa caacctcata ctccgcttcc acagctggcg cagccatttc
     7261 ttcctccatc caagcgcgag cacggcgagc ttgctgctcg gtcatagagt gcatgccttc
     7321 agcgcccacc gcaccacaaa cagcagtcat accctcagcc ggagtgatgc cgaagtgttc
     7381 gcaatatggc ttgaagagtt cgcggattgg ctggagatcc actggttgct ccgcctgagc
     7441 gcgcacctgt tctggctgtt gcgcttgatc catttcctcc gcagcgtaca tgccctgaaa
     7501 agtgtccggg aacgcgaggc gccaagcagc agccttcgcg catttttcga tcataacacc
     7561 cggcattttc gcccaattag atttgccggt ggaatagtcg tcgagcgcca cctcagcata
     7621 cgcagtctcg cggccatcct taaagcgaac ttccgcccaa ccaccgatga gctcctcacc
     7681 cgcctttttg tagcacgcag caccacgttt atgcacaaca tcaccgtcac gcagaacaac
     7741 cacaccagat ttgataccat catagttagc atcacgacac gcgcgacggt taaaaacttg
     7801 ataggaagta atcatagaag ctggagcacc gccgtatttc accaggtaag cgtctttcac
     7861 aaacggattc agacgttgag tctgacagag ttgcagaaag aggcccacct ctttttcagt
     7921 agcgttctca cagatataac ggcgaacatc gtctggagta acttgaaccg ccaggccaga
     7981 gtcgtcagtg aacttcacga tttggttcat tccaaacctc cttaaagtta aacaaaatta
     8041 tttcgatcga gtgcggccgc ggatcatgca ggtggcgtgg gagcctcata ctaagcaaag
     8101 cctcaagttg ctttcgcatt gcaccgtgga gggggtgaag agctattcgt aagggacagg
     8161 ttgaagctac aacggctcac actcgctctt cacttcctcc acggtgccct tctttggtgg
     8221 gtcttggcag aacgggcatg atacacaccc tcgcctgagg ctcccacatc acctgctcta
     8281 gcctcatatg aacggtacta cggaccaaaa agattttcca cctcgatatt gtcagcagct
     8341 actcgccggt caacatcggc agcatatgat aggtgaccat taaccttgct gaggttttta
     8401 gtcaactttc cgtgagcggc aagatgacgg taggcgcgta gcttattacg ataaccctta
     8461 gtaagttttg gcgttcccgt atgaaccaaa agcccatgaa ctttcaggcg agcaggacta
     8521 acggataatt ttgttttccc tggtgctagc tcccatggtg atgcatggaa aacaccagca
     8581 acatcgtccg tcaagcctga cggaacctca gcactccccg aaaatgttac atcgtcagca
     8641 tatcttgtgt aattgcaccc atatttttct gcaaccctga gtagctgctt atcaagatca
     8701 tctgcacata tgttagacaa cactggactg gctggagacc cttgagcaag cccacctcta
     8761 aaacagcaca gacttgatac caaagcggct tggtcgtgat tgtatccgag ccgctgcaag
     8821 cactctttaa cataaatgct aggtgtagat gggaaaaatt cttgaatatc acaagagaat
     8881 acccatttag catttttatg cttgatagca gcgtcaatat aggtgcgtcc ggcaacaaac
     8941 ccatatacgc aatcagccgg cctccatgcg tctgcaagcc gaacagacag ccatttctga
     9001 aaaattttca gtaccaccct tggggcatga atctcacgct gcccattccc tttgggtata
     9061 ttaaatgtcc tgtaatacct atggggctta tttataattg cccagactat cccagggttc
     9121 accccaaaaa tggcggcaac aacagatcca gaaacaaccg gcggcaaatt tagcgcggca
     9181 agtcgattaa cttcctgcaa ttcagtatct gtgatctcgc cttctagcga tttaataaaa
     9241 tcgctaagcg tcggaaaaac cacataggga ttggaaaaga aatagggtgg gagcctcata
     9301 tgtatatctc cttcgaattc ggatcctaga ggttgcataa agcctaaggg gtaggcctta
     9361 ctagagatag ccattttttg cactcctgta tccgcttctt gcaaggctgg acttatccct
     9421 atcaaaccgg acactgcact ttatgctggt tatgctggct gcatggggca ttagttgggg
     9481 agccagattt gtcatggagc aggccgttct gctttatgga tcaggaaaaa actatttgtt
     9541 cttcagtcat ggtactgttc tgatgtacct gctgtgtgtt ttcctggtat accgccgttg
     9601 gatagctccg ctaccggtcg ttggtcagat caagctaatt cccatgcatg gtgaaaacgg
     9661 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     9721 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     9781 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     9841 attc
//
