LOCUS       sequence-482350-       10042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eas1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Eas1
FEATURES             Location/Qualifiers
     source          1..10042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             126..134
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1580..1674
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1795..1814)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1806..2621
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2788..2896
                     /note="incP origin of transfer"
     CDS             complement(2907..3758)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3745..4584)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5095..6066)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6173..6567
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6605..6691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6716..7681)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        8581..8661
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 caacctgtcc cttacgaata gctcttcacc cctcacttac gtgaggtgaa gtaagtgact
       61 caatggaacc ccagccacct gcatgatccg cggccgcact cgatcgaaat aattttgttt
      121 aactttaagg aggtttggaa tgaaccaaat cgtgaagttc actgacgact ctggcctggc
      181 ggttcaagtt actccagacg atgttcgccg ttatatctgt gagaacgcta ctgaaaaaga
      241 ggtgggcctc tttctgcaac tctgtcagac tcaacgtctg aatccgtttg tgaaagacgc
      301 ttacctggtg aaatacggcg gtgctccagc ttctatgatt acttcctatc aagtttttaa
      361 ccgtcgcgcg tgtcgtgatg ctaactatga tggtatcaaa tctggtgtgg ttgttctgcg
      421 tgacggtgat gttgtgcata aacgtggtgc tgcgtgctac aaaaaggcgg gtgaggagct
      481 catcggtggt tgggcggaag ttcgctttaa ggatggccgc gagactgcgt atgctgaggt
      541 ggcgctcgac gactattcca ccggcaaatc taattgggcg aaaatgccgg gtgttatgat
      601 cgaaaaatgc gcgaaggctg ctgcttggcg cctcgcgttc ccggacactt ttcagggcat
      661 gtacgctgcg gaggaaatgg atcaagcgca acagccagaa caggtgcgcg ctcaggcgga
      721 gcaaccagtg gatctccagc caatccgcga actcttcaag ccatattgcg aacacttcgg
      781 catcactccg gctgagggta tgactgctgt ttgtggtgcg gtgggcgctg aaggcatgca
      841 ctctatgacc gagcagcaag ctcgccgtgc tcgcgcttgg atggaggaag aaatggctgc
      901 gccagctgtg gaagcggagt atgaggttgt tgacgagggc gaggtgtttt aaggatccag
      961 gagaatacac atggccagca gaggcgtaaa caaggttatt ctcgttggta atctgggtca
     1021 ggacccggaa gtacgctaca tgccaaatgg tggcgcagtt gccaacatta cgctggctac
     1081 ttccgaatcc tggcgtgata aagcgaccgg cgagatgaaa gagcagactg aatggcaccg
     1141 cgttgtgctg ttcggcaaac tggcagaagt ggccagcgaa tatctgcgta aaggttctca
     1201 ggtttatatc gaaggtcagc tgcgtacccg taaatggacc gatcaatccg gtcaggatcg
     1261 ctacaccaca gaagtcgtgg tgaacgttgg cggcaccatg cagatgctgg gtggtcgtca
     1321 gggtggtggc gctccggcag gtggtaatat cggtggtggt cagccgcagg gcggttgggg
     1381 tcagcctcag cagccgcagg gtggcaatca gttcagcggc ggcgcgcagt ctcgcccgca
     1441 gcagtccgct ccggcagcgc cgtctaacga gccgccgatg gactttgatg atgacattcc
     1501 gttctgatct agagtcgacc tgcaggcatg caagcttgcg gccgcgtcgt gactgggaaa
     1561 accctggcga ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat
     1621 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg
     1681 gtccccaata attacgattt aaatttgtgt ctcaaaatct ctgatgttac attgcacaag
     1741 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1801 gtgttatgag ccatattcag cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca
     1861 tggatgcgga tctgtatggc tataaatggg cgcgtgataa cgtgggtcag agcggcgcga
     1921 ccatttatcg tctgtatggc aaaccggatg cgccggaact gtttctgaaa catggcaaag
     1981 gcagcgtggc gaacgatgtg accgatgaaa tggtgcgtct gaactggctg accgaattta
     2041 tgccgctgcc gaccattaaa cattttattc gcaccccgga tgatgcgtgg ctgctgacca
     2101 ccgcgattcc gggcaaaacc gcgtttcagg tgctggaaga atatccggat agcggcgaaa
     2161 acattgtgga tgcgctggcc gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact
     2221 gcccgtttaa cagcgatcgt gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg
     2281 gcctggtgga tgcgagcgat tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt
     2341 ggaaagaaat gcataaactg ctgccgttta gcccggatag cgtggtgacc cacggcgatt
     2401 ttagcctgga taacctgatt ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc
     2461 gtgtgggcat tgcggatcgt tatcaggatc tggccattct gtggaactgc ctgggcgaat
     2521 ttagcccgag cctgcaaaaa cgtctgtttc agaaatatgg cattgataat ccggatatga
     2581 acaaactgca atttcatctg atgctggatg aatttttcta ataattaatt ggaccgcggt
     2641 ccgcgcgttg tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
     2701 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     2761 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     2821 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     2881 tctgcgaggc tggccgtagg ccggcctcag cctgccgcct tgggccgggt gatgtcgtac
     2941 ttgcccgccg cgaactcggt taccgtccag cccagcgcga ccagctccgg caacgcctcg
     3001 cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg cctctgacgg ccagacatag
     3061 ccgcacaagg tatctatgga agccttgccg gttttgccgg ggtcgatcca gccacacagc
     3121 cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc gcacctcgtc catgctgatg
     3181 cgcacatgct ggccgccacc catgacggcc tgcgcgatca aggggttcag ggccacgtac
     3241 aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca gccgaaaccc ctgccgcttg
     3301 cggccattct gggcgatgat ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc
     3361 ttgagcgccc caccactatc gacctctgcc ccgatttcct ttgccagcgc ccgatagcta
     3421 cctttgacca ccatggcatc agcggtgacg gcctcccact tgggttccag gaacagccgg
     3481 agctgccgtc cgccttcggt cttgggttcc gggccaagca ctaggccatt aggcccagcc
     3541 atggccacca gcccttgcag gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg
     3601 aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc
     3661 tcgccccgct tgagggcacg gaacaggccg ggggccagac agtgcgccgg gtcgtgccgg
     3721 acgtggctga ggctgtgctt gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg
     3781 ctgcctctcc agcacggcgg gcttgagcac cccgccgtca tgccgcctga accaccgatc
     3841 agcgaacggt gcgccatagt tggccttgct cacaccgaag cggacgaaga accggcgctg
     3901 gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc atgctcgaca ggtaggactg
     3961 ccagcggatg ttatcgacca gtaccgagct gccccggctg gcctgctgct ggtcgcctgc
     4021 gcccatcatg gccgcgccct tgctggcatg gtgcaggaac acgatagagc acccggtatc
     4081 ggcggcgatg gcctccatgc gaccgatgac ctgggccatg gggccgctgg cgttttcttc
     4141 ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg cggcggccct cggcggcgcg
     4201 cttgaggccg tcgaaccact ccggggccat gatgttgggc aggctgccga tcagcggctg
     4261 gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc
     4321 gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc
     4381 ggtgggcagt tcgcccacct ccagcagatc cggcccgcct gcaatctgtg cggccagttg
     4441 cagggccagc atggatttac cggcaccacc gggcgacacc agcgccccga ccgtaccggc
     4501 caccatgttg ggcaaaacgt agtccagcgg tggcggcgct gctgcgaacg cctccagaat
     4561 attgataggc ttatgggtag ccattgattg cctcctttgc aggcagttgg tggttaggcg
     4621 ctggcggggt cactaccccc gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc
     4681 gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac gcatcccttt ggccttcatg
     4741 cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc
     4801 ttgtcctttt ggtctttcat atcagtcacc gagaaacttg ccggggccga aaggcttgtc
     4861 ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg ctggccatat cagcgactga
     4921 aaagcggcca gcctcggcct tgtttgacgt ataaccaaag ccaccgggca accaatagcc
     4981 cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc
     5041 cccttctgtg cgtgagtact catagtataa caggcgtgag taccaacgca agcactacat
     5101 gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag
     5161 ctcggcccgc gcaagctgga cgctgggcag acccatgacc ttgctgacgg tgcgctcgat
     5221 gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct gggctggcct cggccatggc
     5281 cttgccgatt tcctcggcac tgcggccccg gctggccagc ttctgcgcgg cgataaagtc
     5341 gcacttgctg aggtcatcac cgaagcgctt gaccagcccg gccatctcgc tgcggtactc
     5401 gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg ggcagttcga ggctggccag
     5461 cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc tgctggccag cctgctgcac
     5521 cagcgccggg ccagcggtgg cggtcttgcc cttggattca cgcagcagca cccacggctg
     5581 ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg aagcccgcca agcggccata
     5641 gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat
     5701 ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg acccatgcct gatagttctt
     5761 cgggctggtt tccactacca gggcaggctc ccggccctcg gctttcatgt catccaggtc
     5821 aaactcgctg aggtcgtcca ccagcaccag accatgccgc tcctgctcgg cgggcctgat
     5881 atacacgtca ttgccctggg cattcatccg cttgagccat ggcgtgttct ggagcacttc
     5941 ggcggctgac cattcccggt tcatcatctg gccggtggtg gcgtccctga cgccgatatc
     6001 gaagcgctca cagcccatgg ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt
     6061 cttcatcggg ccaccaagcg attcccacac attatacgag ccggaagcat aaagtgtagc
     6121 tagatccgaa ggatgagccg ggctgaatga tcgaccgaga caggccctgc ggggctgcac
     6181 acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc
     6241 gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc cccctgccgc
     6301 gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc ggcctctggc
     6361 cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg cgcctagtgg
     6421 attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc cgcccctgct
     6481 gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg
     6541 gggcgtgaca gttattgcag gggttcgtga cagttagggc gcgcccagct gtctagggcg
     6601 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     6661 agtctttcga ctgagccttt cgttttattt gatgccttta attaaacgtt cgtaatcaag
     6721 ccacttcctt tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat
     6781 agttttcagc gaagcgtccc aaatgtaaga agccgtagtc tagggctatc tcagttatac
     6841 tacgcacatt ggcactggga tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc
     6901 ggatgtagtt cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg
     6961 gactcatcat cgccagctcc gctaaccgct caaggctgat attccgtttg agattctcct
     7021 caatgaattg aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt tcacggctga
     7081 cattgctgcc cagcatttcg agcagcttgg aagcgatgat ccccgcatag tgctcttgga
     7141 cccgaggcat cgactttgta tgttccgctt cgtcacaaac taacccgagt agattgataa
     7201 agccatcgag ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt
     7261 gccaattgtt gtcactgcac gcccgatcaa ggaccactga gggcaattta acgataaatt
     7321 tctcgcaatc ttctgaatag gtcaggtcgg cttggtcatc cggattgagc agcaatagtt
     7381 cgcccggcgc aaaatagtgc tcctggccat ggccacgcca caggcaatgg cctttgagta
     7441 ttatttgcag atgataacag gtctctaatc caggcgagat taccctcacg ctaccgccgt
     7501 agctgattcg acacagatcg aggcatccga agattctgtg gtgcagcctg cctgccgggc
     7561 gcccgccctt gggcaggcga atagagtgcg taccgacata ctggttaaca taatcggaga
     7621 ctgcataggg ctcggcgtgg acgaagatct gacttttctc gttcaataag caaaaatcca
     7681 tagttcacgg ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag
     7741 tcgatgaaaa tgcatctcga cgtgatgcgt atacgggtta cccccattgc cacgttgcgc
     7801 catccttttt gcaatcagtg accacttttc caagcaaaaa taacgccaag cagaacgaag
     7861 acgttctttt taagaagcga gaacaccaga agttcgtgct gtcggggcat ggggcgacga
     7921 attggcggat aaaggggatc tgctggatat tacggccttt ttaaagaccg taaagaaaaa
     7981 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     8041 gtaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     8101 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     8161 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     8221 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     8281 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     8341 caccatgcat gggaattagc ttgatctgac caacgaccgg tagcggagct atccaacggc
     8401 ggtataccag gaaaacacac agcaggtaca tcagaacagt accatgactg aagaacaaat
     8461 agttttttcc tgatccataa agcagaacgg cctgctccat gacaaatctg gctccccaac
     8521 taatgcccca tgcagccagc ataaccagca taaagtgcag tgtccggttt gatagggata
     8581 agtccagcct tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc
     8641 taccccttag gctttatgca acctctagga tccgaattcg aaggagatat acatatgact
     8701 caatggaacc ccaacctttt tcgtaaggaa ggtttatcta aaggatatga ttctcactat
     8761 ttgacacgct tagtcgagca aggggaaaca attaatgaaa gaaaccttcc tgtagttttt
     8821 tctctagcac atttagccaa tttatctcat actttgtatt ctgacttaca cggttttgta
     8881 agccgtaacg accttgaacg aaaagacttc ccatataaaa acttttcaat ttcgaaaagg
     8941 accggaggaa aacgttggat ttccattcct actcctgcac ttatggctgt gcagtattgg
     9001 ataaacaaaa atatacttaa tagaattatt cctcacgctg cagcatatgc ttatgtcccg
     9061 aaaagaaaaa taatccagca tgcacaacgt cattgcggtt caggctggtt gattaaaatt
     9121 gatattaaag atttttttgg caatataagt gagcgccaag tttttgatgt ttttctaaat
     9181 ctaggatatc caaaactctt atccttcgaa atggcaaggt tgtgcacacg agtaaccccc
     9241 aaaagaaagg gaaagaggtg gtgtaacact aataatgaac atacaatcac tggttatgat
     9301 tcaaaatttg tgggcagctt accacaagga gcacccacaa gccctgcatt atcgaatctg
     9361 gtttgtatcg gtctcgataa gcaactaaca tcgcttgcaa tatctgaagg cgctacttac
     9421 agccgttatg ctgacgatat atgtttttct ttaccaatgg gatgtagaaa aagcgcatta
     9481 cacatcaaaa aagagatatc taaaattctt catggatatg gtttttctga taacagcaaa
     9541 aaaacgaggc taattccgcc aggggctaga aaagttctta ctgggcttaa tgtaaataat
     9601 gacacaccaa gaatacctaa agaaataagg gattcagtta gaatgcatct atattatgca
     9661 aaaaaaaatg gtatcgcaac tcattgtaaa acgcgtggtt ttcgttcagt tattggattc
     9721 tacaactatc tatcaggagt aataggatat atttattcca tagactcaaa catggggaat
     9781 aagttccgga atgaattcaa ctcattacct tgggtagatt tttctttata ataccgttca
     9841 tatgaggcta gagcaggtga ttggggttcc gtcgtgaaac ttagttgtgg tagatacata
     9901 tatagtgtat caactgccat tggcagccta cgattccgtt tgaatggttt gacaggatgc
     9961 caacgagcac ggattccgtc gctctttgta gtagccttcg ccagagtgag aagtgaagag
    10021 cgagtgtgag ccgttgtagc tt
//
