LOCUS       sequence-482353-        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Ban1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Ban1
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1377..1385
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(2745..2825)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             3725..4690
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     terminator      4715..4801
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4839..5233
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5340..6311
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6822..7661
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7648..8499
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8510..8618
                     /note="incP origin of transfer"
     CDS             complement(8785..9600)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9592..9611
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(9732..9826)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 agatcagaac ggaatgtcat catcaaagtc catcggcggc tcgttagacg gcgctgccgg
       61 agcggactgc tgcgggcgag actgcgcgcc gccgctgaac tgattgccac cctgcggctg
      121 ctgaggctga ccccaaccgc cctgcggctg accaccaccg atattaccac ctgccggagc
      181 gccaccaccc tgacgaccac ccagcatctg catggtgccg ccaacgttca ccacgacttc
      241 tgtggtgtag cgatcctgac cggattgatc ggtccattta cgggtacgca gctgaccttc
      301 gatataaacc tgagaacctt tacgcagata ttcgctggcc acttctgcca gtttgccgaa
      361 cagcacaacg cggtgccatt cagtctgctc tttcatctcg ccggtcgctt tatcacgcca
      421 ggattcggaa gtagccagcg taatgttggc aactgcgcca ccatttggca tgtagcgtac
      481 ttccgggtcc tgacccagat taccaacgag aataaccttg tttacgcctc tgctggccat
      541 gtgtattctc ctggatcctt aaaacacctc gccctcgtca acaacctcat actccgcttc
      601 cacagctggc gcagccattt cttcctccat ccaagcgcga gcacggcgag cttgctgctc
      661 ggtcatagag tgcatgcctt cagcgcccac cgcaccacaa acagcagtca taccctcagc
      721 cggagtgatg ccgaagtgtt cgcaatatgg cttgaagagt tcgcggattg gctggagatc
      781 cactggttgc tccgcctgag cgcgcacctg ttctggctgt tgcgcttgat ccatttcctc
      841 cgcagcgtac atgccctgaa aagtgtccgg gaacgcgagg cgccaagcag cagccttcgc
      901 gcatttttcg atcataacac ccggcatttt cgcccaatta gatttgccgg tggaatagtc
      961 gtcgagcgcc acctcagcat acgcagtctc gcggccatcc ttaaagcgaa cttccgccca
     1021 accaccgatg agctcctcac ccgccttttt gtagcacgca gcaccacgtt tatgcacaac
     1081 atcaccgtca cgcagaacaa ccacaccaga tttgatacca tcatagttag catcacgaca
     1141 cgcgcgacgg ttaaaaactt gataggaagt aatcatagaa gctggagcac cgccgtattt
     1201 caccaggtaa gcgtctttca caaacggatt cagacgttga gtctgacaga gttgcagaaa
     1261 gaggcccacc tctttttcag tagcgttctc acagatataa cggcgaacat cgtctggagt
     1321 aacttgaacc gccaggccag agtcgtcagt gaacttcacg atttggttca ttccaaacct
     1381 ccttaaagtt aaacaaaatt atttcgatcg agtgcggccg cggatcatgc aggtggcatg
     1441 tttagtgact acttcacctt taaattaagg gtgaagagct attcgtaagg gacaggttga
     1501 agctacaacg gctcacactc gctcttcact tttcttaatt taaaggtgag gatggttctg
     1561 caattttggg cagaatcatc attatggttt tgaaataaga attaaataca tacttacttc
     1621 gattagcact tgctaaaagt ctagtcacca ctaaacatat cacctgctct agcctcatat
     1681 gaacggtatt aatcccaatc taattcctga attttcttta tcaactctat acccttgctt
     1741 tgatcaacca tataaatgaa tcttgctaac ccaattaagt accctttata attagcaata
     1801 gtaataagcc catgatattt caaatgttca tttacaccaa actttttaca ataataaatt
     1861 tctttattca gtcttcgaat atatttttgt ggaatcctta caccttgtgg tgtgataact
     1921 agcccagtta cgattttcgt atgcctatcc ttcaatagtc tagttttctt attattgata
     1981 ataaaatcat acttactcaa tacacaggac actatctcct ttattttctt tactaattta
     2041 agatacccct ttttttggac acctgaaaaa gtaatatcat ctgcatatct agaatatcga
     2101 atgttatatc gactagctaa ttgttgaaat tcttcatcaa tctcacgaaa gaaaatattg
     2161 gacagcattg gacttgttgg aaaaccttgt accagctcat tattcacagt tgttaataaa
     2221 ctaaatgctt cactaactgc atctgaataa ccaattccta tgaatatttt cttaatctct
     2281 ttatatttaa tagaaggaaa aaaatctttg atatccgttg aaaacaccca aaaaggttct
     2341 tgatgttgat gaacttcggc attcgttaga atggatttac tcttcacaaa gccgtgtgca
     2401 caagctgaca cttcaatttt atttaggata ttcttaagta tccatttttg aatcgtcttt
     2461 agtttataat ttggcttcca tacttctctt ggttctccat ttcgtttgct gacataataa
     2521 tgattggtat tgtctatcac gaacttctcc aaatcatcat ctggtaactc aaaaagtctc
     2581 aaaatctgat tgatgtcaaa gataactgga caatggttag aatgtatatc tttagcatat
     2641 tgcataaaga attttttttc ttcctccgat aaatcgcttg atatgatgtt atgtaaaact
     2701 tgcttaatca tatgtatatc tccttcgaat tcggatccta gaggttgcat aaagcctaag
     2761 gggtaggcct tactagagat agccattttt tgcactcctg tatccgcttc ttgcaaggct
     2821 ggacttatcc ctatcaaacc ggacactgca ctttatgctg gttatgctgg ctgcatgggg
     2881 cattagttgg ggagccagat ttgtcatgga gcaggccgtt ctgctttatg gatcaggaaa
     2941 aaactatttg ttcttcagtc atggtactgt tctgatgtac ctgctgtgtg ttttcctggt
     3001 ataccgccgt tggatagctc cgctaccggt cgttggtcag atcaagctaa ttcccatgca
     3061 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     3121 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     3181 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     3241 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     3301 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgta
     3361 attacggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     3421 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagcagatcc cctttatccg
     3481 ccaattcgtc gccccatgcc ccgacagcac gaacttctgg tgttctcgct tcttaaaaag
     3541 aacgtcttcg ttctgcttgg cgttattttt gcttggaaaa gtggtcactg attgcaaaaa
     3601 ggatggcgca acgtggcaat gggggtaacc cgtatacgca tcacgtcgag atgcattttc
     3661 atcgacttgg cgcctttcta catcacacca agcagcccac attaaaataa gagaaccgtg
     3721 aactatggat ttttgcttat tgaacgagaa aagtcagatc ttcgtccacg ccgagcccta
     3781 tgcagtctcc gattatgtta accagtatgt cggtacgcac tctattcgcc tgcccaaggg
     3841 cgggcgcccg gcaggcaggc tgcaccacag aatcttcgga tgcctcgatc tgtgtcgaat
     3901 cagctacggc ggtagcgtga gggtaatctc gcctggatta gagacctgtt atcatctgca
     3961 aataatactc aaaggccatt gcctgtggcg tggccatggc caggagcact attttgcgcc
     4021 gggcgaacta ttgctgctca atccggatga ccaagccgac ctgacctatt cagaagattg
     4081 cgagaaattt atcgttaaat tgccctcagt ggtccttgat cgggcgtgca gtgacaacaa
     4141 ttggcacaag ccgagggagg gtatccgttt cgccgcgcga cacaatctcc agcaactcga
     4201 tggctttatc aatctactcg ggttagtttg tgacgaagcg gaacatacaa agtcgatgcc
     4261 tcgggtccaa gagcactatg cggggatcat cgcttccaag ctgctcgaaa tgctgggcag
     4321 caatgtcagc cgtgaaattt tcagcaaagg taacccgtct ttcgagcgag tcgttcaatt
     4381 cattgaggag aatctcaaac ggaatatcag ccttgagcgg ttagcggagc tggcgatgat
     4441 gagtccacgc tcgctctaca atttgttcga gaagcacgcc ggcaccacgc cgaagaacta
     4501 catccgcaac cgcaagctcg aaagcatccg cgcctgcttg aacgatccca gtgccaatgt
     4561 gcgtagtata actgagatag ccctagacta cggcttctta catttgggac gcttcgctga
     4621 aaactatagg agcgcgttcg gcgagttgcc ttccgacacc ctgcgtcaat gcaaaaagga
     4681 agtggcttga ttacgaacgt ttaattaaag gcatcaaata aaacgaaagg ctcagtcgaa
     4741 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4801 tccgccgccc tagacagctg ggcgcgccct aactgtcacg aacccctgca ataactgtca
     4861 cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca
     4921 aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga
     4981 ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat
     5041 gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc
     5101 gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga
     5161 aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg
     5221 cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttcgg
     5281 atctagctac actttatgct tccggctcgt ataatgtgtg ggaatcgctt ggtggcccga
     5341 tgaagaacga caggactttg caggccatag gccgacagct caaggccatg ggctgtgagc
     5401 gcttcgatat cggcgtcagg gacgccacca ccggccagat gatgaaccgg gaatggtcag
     5461 ccgccgaagt gctccagaac acgccatggc tcaagcggat gaatgcccag ggcaatgacg
     5521 tgtatatcag gcccgccgag caggagcggc atggtctggt gctggtggac gacctcagcg
     5581 agtttgacct ggatgacatg aaagccgagg gccgggagcc tgccctggta gtggaaacca
     5641 gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg
     5701 ggcagattgc ccggacgctg gccagcgagt acgacgccga cccggccagc gccgacagcc
     5761 gccactatgg ccgcttggcg ggcttcacca accgcaagga caagcacacc acccgcgccg
     5821 gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg
     5881 cgctggtgca gcaggctggc cagcagatcg agcaggccca gcggcagcag gagaaggccc
     5941 gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc
     6001 tggacgagta ccgcagcgag atggccgggc tggtcaagcg cttcggtgat gacctcagca
     6061 agtgcgactt tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg
     6121 gcaaggccat ggccgaggcc agcccagcgc tggcagagcg caagcccggc cacgaagcgg
     6181 attacatcga gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg
     6241 ccgagctggc acgggcaccg gcaccccgcc agcgaggcat ggacaggggc gggccagatt
     6301 tcagcatgta gtgcttgcgt tggtactcac gcctgttata ctatgagtac tcacgcacag
     6361 aagggggttt tatggaatac gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg
     6421 acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc gaggctggcc
     6481 gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg tccttgttcc
     6541 gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa agaccaaaag
     6601 gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc gcgatatgcc
     6661 gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga cgacgaatac
     6721 gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg tagtgacccc
     6781 gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc cataagccta
     6841 tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca
     6901 tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg
     6961 ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg ggcgaactgc
     7021 ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt catcaccgcc
     7081 tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct gacggcctgc
     7141 tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg ttcgacggcc
     7201 tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc cggttccaca
     7261 tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg gaggccatcg
     7321 ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc gcggccatga
     7381 tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc gataacatcc
     7441 gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg
     7501 acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt
     7561 tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc gtgctggaga
     7621 ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca cagcctcagc
     7681 cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg
     7741 ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg caagcggatc
     7801 gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca agggctggtg
     7861 gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga aggcggacgg
     7921 cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc catggtggtc
     7981 aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag tggtggggcg
     8041 ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat cgcccagaat
     8101 ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga ggcggacggg
     8161 cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg cggccagcat
     8221 gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac
     8281 cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat agataccttg
     8341 tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg
     8401 gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg
     8461 ggcaagtacg acatcacccg gcccaaggcg gcaggctgag gccggcctac ggccagcctc
     8521 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     8581 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     8641 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     8701 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aaggacaacg
     8761 cgcggaccgc ggtccaatta attattagaa aaattcatcc agcatcagat gaaattgcag
     8821 tttgttcata tccggattat caatgccata tttctgaaac agacgttttt gcaggctcgg
     8881 gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat ccgcaatgcc
     8941 cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca ggttatccag
     9001 gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt tatgcatttc
     9061 tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc tcgcatccac
     9121 caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat cgctgttaaa
     9181 cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca gcgcatccac
     9241 aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt tgcccggaat
     9301 cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa tggtcggcag
     9361 cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat cgttcgccac
     9421 gctgcctttg ccatgtttca gaaacagttc cggcgcatcc ggtttgccat acagacgata
     9481 aatggtcgcg ccgctctgac ccacgttatc acgcgcccat ttatagccat acagatccgc
     9541 atccatgttg ctgttcagac gcggacggct acagctcgtt tcacgctgaa tatggctcat
     9601 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     9661 tttatcttgt gcaatgtaac atcagagatt ttgagacaca aatttaaatc gtaattattg
     9721 gggacccctg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     9781 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag actagtcgcc
     9841 agggttttcc cagtcacgac gcggccgcaa gcttgcatgc ctgcaggtcg actct
//
