LOCUS       sequence-482363-        9883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Ebu1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Ebu1
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             127..135
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1581..1675
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1796..1815)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1807..2622
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2789..2897
                     /note="incP origin of transfer"
     CDS             complement(2908..3759)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3746..4585)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5096..6067)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6174..6568
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6606..6692)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6717..7682)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        8582..8662
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 caacctgtcc cttacgaata gctcttcacc cagagaactt aggtgtcagg tatagaaagt
       61 agtttctaca agtcgccacc tgcatgatcc gcggccgcac tcgatcgaaa taattttgtt
      121 taactttaag gaggtttgga atgaaccaaa tcgtgaagtt cactgacgac tctggcctgg
      181 cggttcaagt tactccagac gatgttcgcc gttatatctg tgagaacgct actgaaaaag
      241 aggtgggcct ctttctgcaa ctctgtcaga ctcaacgtct gaatccgttt gtgaaagacg
      301 cttacctggt gaaatacggc ggtgctccag cttctatgat tacttcctat caagttttta
      361 accgtcgcgc gtgtcgtgat gctaactatg atggtatcaa atctggtgtg gttgttctgc
      421 gtgacggtga tgttgtgcat aaacgtggtg ctgcgtgcta caaaaaggcg ggtgaggagc
      481 tcatcggtgg ttgggcggaa gttcgcttta aggatggccg cgagactgcg tatgctgagg
      541 tggcgctcga cgactattcc accggcaaat ctaattgggc gaaaatgccg ggtgttatga
      601 tcgaaaaatg cgcgaaggct gctgcttggc gcctcgcgtt cccggacact tttcagggca
      661 tgtacgctgc ggaggaaatg gatcaagcgc aacagccaga acaggtgcgc gctcaggcgg
      721 agcaaccagt ggatctccag ccaatccgcg aactcttcaa gccatattgc gaacacttcg
      781 gcatcactcc ggctgagggt atgactgctg tttgtggtgc ggtgggcgct gaaggcatgc
      841 actctatgac cgagcagcaa gctcgccgtg ctcgcgcttg gatggaggaa gaaatggctg
      901 cgccagctgt ggaagcggag tatgaggttg ttgacgaggg cgaggtgttt taaggatcca
      961 ggagaataca catggccagc agaggcgtaa acaaggttat tctcgttggt aatctgggtc
     1021 aggacccgga agtacgctac atgccaaatg gtggcgcagt tgccaacatt acgctggcta
     1081 cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa agagcagact gaatggcacc
     1141 gcgttgtgct gttcggcaaa ctggcagaag tggccagcga atatctgcgt aaaggttctc
     1201 aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac cgatcaatcc ggtcaggatc
     1261 gctacaccac agaagtcgtg gtgaacgttg gcggcaccat gcagatgctg ggtggtcgtc
     1321 agggtggtgg cgctccggca ggtggtaata tcggtggtgg tcagccgcag ggcggttggg
     1381 gtcagcctca gcagccgcag ggtggcaatc agttcagcgg cggcgcgcag tctcgcccgc
     1441 agcagtccgc tccggcagcg ccgtctaacg agccgccgat ggactttgat gatgacattc
     1501 cgttctgatc tagagtcgac ctgcaggcat gcaagcttgc ggccgcgtcg tgactgggaa
     1561 aaccctggcg actagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
     1621 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagg
     1681 ggtccccaat aattacgatt taaatttgtg tctcaaaatc tctgatgtta cattgcacaa
     1741 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1801 ggtgttatga gccatattca gcgtgaaacg agctgtagcc gtccgcgtct gaacagcaac
     1861 atggatgcgg atctgtatgg ctataaatgg gcgcgtgata acgtgggtca gagcggcgcg
     1921 accatttatc gtctgtatgg caaaccggat gcgccggaac tgtttctgaa acatggcaaa
     1981 ggcagcgtgg cgaacgatgt gaccgatgaa atggtgcgtc tgaactggct gaccgaattt
     2041 atgccgctgc cgaccattaa acattttatt cgcaccccgg atgatgcgtg gctgctgacc
     2101 accgcgattc cgggcaaaac cgcgtttcag gtgctggaag aatatccgga tagcggcgaa
     2161 aacattgtgg atgcgctggc cgtgtttctg cgtcgtctgc atagcattcc ggtgtgcaac
     2221 tgcccgttta acagcgatcg tgtgtttcgt ctggcccagg cgcagagccg tatgaacaac
     2281 ggcctggtgg atgcgagcga ttttgatgat gaacgtaacg gctggccggt ggaacaggtg
     2341 tggaaagaaa tgcataaact gctgccgttt agcccggata gcgtggtgac ccacggcgat
     2401 tttagcctgg ataacctgat tttcgatgaa ggcaaactga ttggctgcat tgatgtgggc
     2461 cgtgtgggca ttgcggatcg ttatcaggat ctggccattc tgtggaactg cctgggcgaa
     2521 tttagcccga gcctgcaaaa acgtctgttt cagaaatatg gcattgataa tccggatatg
     2581 aacaaactgc aatttcatct gatgctggat gaatttttct aataattaat tggaccgcgg
     2641 tccgcgcgtt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     2701 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     2761 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     2821 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     2881 ctctgcgagg ctggccgtag gccggcctca gcctgccgcc ttgggccggg tgatgtcgta
     2941 cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg accagctccg gcaacgcctc
     3001 gcgcacccgc tggcggcgct tgcgcatggt cgaaccactg gcctctgacg gccagacata
     3061 gccgcacaag gtatctatgg aagccttgcc ggttttgccg gggtcgatcc agccacacag
     3121 ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat
     3181 gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc aaggggttca gggccacgta
     3241 caggcgcccg tccgcctcgt cgctggcgta ctccgacagc agccgaaacc cctgccgctt
     3301 gcggccattc tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg
     3361 cttgagcgcc ccaccactat cgacctctgc cccgatttcc tttgccagcg cccgatagct
     3421 acctttgacc accatggcat cagcggtgac ggcctcccac ttgggttcca ggaacagccg
     3481 gagctgccgt ccgccttcgg tcttgggttc cgggccaagc actaggccat taggcccagc
     3541 catggccacc agcccttgca ggatgcgcag atcatcagcg cccagcggct ccgggccgct
     3601 gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg
     3661 ctcgccccgc ttgagggcac ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg
     3721 gacgtggctg aggctgtgct tgttcttagg cttcaccacg gggcaccccc ttgctcttgc
     3781 gctgcctctc cagcacggcg ggcttgagca ccccgccgtc atgccgcctg aaccaccgat
     3841 cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa gcggacgaag aaccggcgct
     3901 ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt catgctcgac aggtaggact
     3961 gccagcggat gttatcgacc agtaccgagc tgccccggct ggcctgctgc tggtcgcctg
     4021 cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag cacccggtat
     4081 cggcggcgat ggcctccatg cgaccgatga cctgggccat ggggccgctg gcgttttctt
     4141 cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc
     4201 gcttgaggcc gtcgaaccac tccggggcca tgatgttggg caggctgccg atcagcggct
     4261 ggatcagcag gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc gccccaaggg
     4321 cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc
     4381 cggtgggcag ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt gcggccagtt
     4441 gcagggccag catggattta ccggcaccac cgggcgacac cagcgccccg accgtaccgg
     4501 ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa
     4561 tattgatagg cttatgggta gccattgatt gcctcctttg caggcagttg gtggttaggc
     4621 gctggcgggg tcactacccc cgccctgcgc cgctctgagt tcttccaggc actcgcgcag
     4681 cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt tggccttcat
     4741 gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt cgccggtctg
     4801 cttgtccttt tggtctttca tatcagtcac cgagaaactt gccggggccg aaaggcttgt
     4861 cttcgcggaa caaggacaag gtgcagccgt caaggttaag gctggccata tcagcgactg
     4921 aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa gccaccgggc aaccaatagc
     4981 ccttgtcact tttgatcagg tagaccgacc ctgaagcgct tttttcgtat tccataaaac
     5041 ccccttctgt gcgtgagtac tcatagtata acaggcgtga gtaccaacgc aagcactaca
     5101 tgctgaaatc tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca
     5161 gctcggcccg cgcaagctgg acgctgggca gacccatgac cttgctgacg gtgcgctcga
     5221 tgtaatccgc ttcgtggccg ggcttgcgct ctgccagcgc tgggctggcc tcggccatgg
     5281 ccttgccgat ttcctcggca ctgcggcccc ggctggccag cttctgcgcg gcgataaagt
     5341 cgcacttgct gaggtcatca ccgaagcgct tgaccagccc ggccatctcg ctgcggtact
     5401 cgtccagcgc cgtgcgccgg tggcggctaa gctgccgctc gggcagttcg aggctggcca
     5461 gcctgcgggc cttctcctgc tgccgctggg cctgctcgat ctgctggcca gcctgctgca
     5521 ccagcgccgg gccagcggtg gcggtcttgc ccttggattc acgcagcagc acccacggct
     5581 gataaccggc gcgggtggtg tgcttgtcct tgcggttggt gaagcccgcc aagcggccat
     5641 agtggcggct gtcggcgctg gccgggtcgg cgtcgtactc gctggccagc gtccgggcaa
     5701 tctgcccccg aagttcaccg cctgcggcgt cggccacctt gacccatgcc tgatagttct
     5761 tcgggctggt ttccactacc agggcaggct cccggccctc ggctttcatg tcatccaggt
     5821 caaactcgct gaggtcgtcc accagcacca gaccatgccg ctcctgctcg gcgggcctga
     5881 tatacacgtc attgccctgg gcattcatcc gcttgagcca tggcgtgttc tggagcactt
     5941 cggcggctga ccattcccgg ttcatcatct ggccggtggt ggcgtccctg acgccgatat
     6001 cgaagcgctc acagcccatg gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt
     6061 tcttcatcgg gccaccaagc gattcccaca cattatacga gccggaagca taaagtgtag
     6121 ctagatccga aggatgagcc gggctgaatg atcgaccgag acaggccctg cggggctgca
     6181 cacgcgcccc cacccttcgg gtagggggaa aggccgctaa agcggctaaa agcgctccag
     6241 cgtatttctg cggggtttgg tgtggggttt agcgggcttt gcccgccttt ccccctgccg
     6301 cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag accagctatc cggcctctgg
     6361 ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc gctgataacc gcgcctagtg
     6421 gattattctt agataatcat ggatggattt ttccaacacc ccgccagccc ccgcccctgc
     6481 tgggtttgca ggtttggggg cgtgacagtt attgcagggg ttcgtgacag ttattgcagg
     6541 ggggcgtgac agttattgca ggggttcgtg acagttaggg cgcgcccagc tgtctagggc
     6601 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     6661 cagtctttcg actgagcctt tcgttttatt tgatgccttt aattaaacgt tcgtaatcaa
     6721 gccacttcct ttttgcattg acgcagggtg tcggaaggca actcgccgaa cgcgctccta
     6781 tagttttcag cgaagcgtcc caaatgtaag aagccgtagt ctagggctat ctcagttata
     6841 ctacgcacat tggcactggg atcgttcaag caggcgcgga tgctttcgag cttgcggttg
     6901 cggatgtagt tcttcggcgt ggtgccggcg tgcttctcga acaaattgta gagcgagcgt
     6961 ggactcatca tcgccagctc cgctaaccgc tcaaggctga tattccgttt gagattctcc
     7021 tcaatgaatt gaacgactcg ctcgaaagac gggttacctt tgctgaaaat ttcacggctg
     7081 acattgctgc ccagcatttc gagcagcttg gaagcgatga tccccgcata gtgctcttgg
     7141 acccgaggca tcgactttgt atgttccgct tcgtcacaaa ctaacccgag tagattgata
     7201 aagccatcga gttgctggag attgtgtcgc gcggcgaaac ggataccctc cctcggcttg
     7261 tgccaattgt tgtcactgca cgcccgatca aggaccactg agggcaattt aacgataaat
     7321 ttctcgcaat cttctgaata ggtcaggtcg gcttggtcat ccggattgag cagcaatagt
     7381 tcgcccggcg caaaatagtg ctcctggcca tggccacgcc acaggcaatg gcctttgagt
     7441 attatttgca gatgataaca ggtctctaat ccaggcgaga ttaccctcac gctaccgccg
     7501 tagctgattc gacacagatc gaggcatccg aagattctgt ggtgcagcct gcctgccggg
     7561 cgcccgccct tgggcaggcg aatagagtgc gtaccgacat actggttaac ataatcggag
     7621 actgcatagg gctcggcgtg gacgaagatc tgacttttct cgttcaataa gcaaaaatcc
     7681 atagttcacg gttctcttat tttaatgtgg gctgcttggt gtgatgtaga aaggcgccaa
     7741 gtcgatgaaa atgcatctcg acgtgatgcg tatacgggtt acccccattg ccacgttgcg
     7801 ccatcctttt tgcaatcagt gaccactttt ccaagcaaaa ataacgccaa gcagaacgaa
     7861 gacgttcttt ttaagaagcg agaacaccag aagttcgtgc tgtcggggca tggggcgacg
     7921 aattggcgga taaaggggat ctgctggata ttacggcctt tttaaagacc gtaaagaaaa
     7981 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     8041 cgtaattacg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     8101 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     8161 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     8221 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     8281 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     8341 tcaccatgca tgggaattag cttgatctga ccaacgaccg gtagcggagc tatccaacgg
     8401 cggtatacca ggaaaacaca cagcaggtac atcagaacag taccatgact gaagaacaaa
     8461 tagttttttc ctgatccata aagcagaacg gcctgctcca tgacaaatct ggctccccaa
     8521 ctaatgcccc atgcagccag cataaccagc ataaagtgca gtgtccggtt tgatagggat
     8581 aagtccagcc ttgcaagaag cggatacagg agtgcaaaaa atggctatct ctagtaaggc
     8641 ctacccctta ggctttatgc aacctctagg atccgaattc gaaggagata tacatatgag
     8701 acgtgaatgg gaagattttt atcttgaaaa aggtataaat accaatttta tatctactta
     8761 tcttgattat attaaaactt tagagtctcg aaacattccc gtaatctttg aagttgaaca
     8821 cttatctaag cttattggaa taaaataccc tgaaatcaac gacattgttt ttggaacaaa
     8881 atcgttttat cgcgaattta aaataccgaa aaagaaagga ggatatcgaa aaatatcttc
     8941 accttatcca tcgctacgta agtgtcagac atggattcat gaaaaattgc tatattcttt
     9001 acgaactcat aaaaatgcgt ttgcatataa gaaaggaaaa tcaattatag ataatgcaga
     9061 acagcatttg aataatcatt tcttacttaa aattgacatc aaagattttt ttcctagcat
     9121 accaataaat tgggtaatta aattatttga ttctctggga tatgcgaaaa atgtatctta
     9181 tgcactagca tcattatgtt gtcttgacgg ttgcttacct caaggcggca tcactagccc
     9241 ttcactaagt aatatattac tttatccatt agatgaacga ctgtcaaaat tggcgttaaa
     9301 atttaatttg aaatatagcc gatatgccga tgatatggtt ttttctggcg aagcgattcc
     9361 gtcaaaattt attagttatg taactgacat aattgaaagc tatggtttta ttttaaataa
     9421 agaaaaaacg aaactgctga acaacacaaa acaaaaaata gtgacgggaa ttgacgttag
     9481 taaagataag ttacaattac cgagaaaaac caggcgtgaa attagaaaaa ccatgcatta
     9541 tattaaaaaa tacgggattt ttagtcacat tgataatgaa aaaattaaag accctaatta
     9601 cataaaaagt ttagaaggaa aaattcgttt ttggttacaa gttgagccag ataaccaaga
     9661 ggccagaagt tacattgaac acattaatca aatgaagtaa taccgttcat atgaggctag
     9721 agcaggtgat gacttgtaga aactagcacg ttggagtgta gtcgctcctt ggttaagggt
     9781 cactttaacc acgactatac ggataactcc gtatagtcgc ttcgaatagc gttgctattc
     9841 tcgcggttct ttaagtgaag agcgagtgtg agccgttgta gct
//
