LOCUS       sequence-482371-       10082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Hcl recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Hcl1
FEATURES             Location/Qualifiers
     source          1..10082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      791..1185
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(1223..1309)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1334..2299)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        3199..3279
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             4826..4834
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      6280..6374
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6495..6514)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6506..7321
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7488..7596
                     /note="incP origin of transfer"
     CDS             complement(7607..8458)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8445..9284)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(9795..10082,1..684))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARA*LARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 catctcgctg cggtactcgt ccagcgccgt gcgccggtgg cggctaagct gccgctcggg
       61 cagttcgagg ctggccagcc tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg
      121 ctggccagcc tgctgcacca gcgccgggcc agcggtggcg gtcttgccct tggattcacg
      181 cagcagcacc cacggctgat aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa
      241 gcccgccaag cggccatagt ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct
      301 ggccagcgtc cgggcaatct gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac
      361 ccatgcctga tagttcttcg ggctggtttc cactaccagg gcaggctccc ggccctcggc
      421 tttcatgtca tccaggtcaa actcgctgag gtcgtccacc agcaccagac catgccgctc
      481 ctgctcggcg ggcctgatat acacgtcatt gccctgggca ttcatccgct tgagccatgg
      541 cgtgttctgg agcacttcgg cggctgacca ttcccggttc atcatctggc cggtggtggc
      601 gtccctgacg ccgatatcga agcgctcaca gcccatggcc ttgagctgtc ggcctatggc
      661 ctgcaaagtc ctgtcgttct tcatcgggcc accaagcgat tcccacacat tatacgagcc
      721 ggaagcataa agtgtagcta gatccgaagg atgagccggg ctgaatgatc gaccgagaca
      781 ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc
      841 ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc
      901 cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc
      961 agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct
     1021 gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg
     1081 ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc
     1141 gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagggcgc
     1201 gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1261 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat
     1321 taaacgttcg taatcaagcc acttcctttt tgcattgacg cagggtgtcg gaaggcaact
     1381 cgccgaacgc gctcctatag ttttcagcga agcgtcccaa atgtaagaag ccgtagtcta
     1441 gggctatctc agttatacta cgcacattgg cactgggatc gttcaagcag gcgcggatgc
     1501 tttcgagctt gcggttgcgg atgtagttct tcggcgtggt gccggcgtgc ttctcgaaca
     1561 aattgtagag cgagcgtgga ctcatcatcg ccagctccgc taaccgctca aggctgatat
     1621 tccgtttgag attctcctca atgaattgaa cgactcgctc gaaagacggg ttacctttgc
     1681 tgaaaatttc acggctgaca ttgctgccca gcatttcgag cagcttggaa gcgatgatcc
     1741 ccgcatagtg ctcttggacc cgaggcatcg actttgtatg ttccgcttcg tcacaaacta
     1801 acccgagtag attgataaag ccatcgagtt gctggagatt gtgtcgcgcg gcgaaacgga
     1861 taccctccct cggcttgtgc caattgttgt cactgcacgc ccgatcaagg accactgagg
     1921 gcaatttaac gataaatttc tcgcaatctt ctgaataggt caggtcggct tggtcatccg
     1981 gattgagcag caatagttcg cccggcgcaa aatagtgctc ctggccatgg ccacgccaca
     2041 ggcaatggcc tttgagtatt atttgcagat gataacaggt ctctaatcca ggcgagatta
     2101 ccctcacgct accgccgtag ctgattcgac acagatcgag gcatccgaag attctgtggt
     2161 gcagcctgcc tgccgggcgc ccgcccttgg gcaggcgaat agagtgcgta ccgacatact
     2221 ggttaacata atcggagact gcatagggct cggcgtggac gaagatctga cttttctcgt
     2281 tcaataagca aaaatccata gttcacggtt ctcttatttt aatgtgggct gcttggtgtg
     2341 atgtagaaag gcgccaagtc gatgaaaatg catctcgacg tgatgcgtat acgggttacc
     2401 cccattgcca cgttgcgcca tcctttttgc aatcagtgac cacttttcca agcaaaaata
     2461 acgccaagca gaacgaagac gttcttttta agaagcgaga acaccagaag ttcgtgctgt
     2521 cggggcatgg ggcgacgaat tggcggataa aggggatctg ctggatatta cggccttttt
     2581 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     2641 cctgatgaat gctcatccgt aattacgtat ggcaatgaaa gacggtgagc tggtgatatg
     2701 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     2761 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     2821 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     2881 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2941 cttcttcgcc cccgttttca ccatgcatgg gaattagctt gatctgacca acgaccggta
     3001 gcggagctat ccaacggcgg tataccagga aaacacacag caggtacatc agaacagtac
     3061 catgactgaa gaacaaatag ttttttcctg atccataaag cagaacggcc tgctccatga
     3121 caaatctggc tccccaacta atgccccatg cagccagcat aaccagcata aagtgcagtg
     3181 tccggtttga tagggataag tccagccttg caagaagcgg atacaggagt gcaaaaaatg
     3241 gctatctcta gtaaggccta ccccttaggc tttatgcaac ctctaggatc cgaattcgaa
     3301 ggagatatac atttgtcaat gattcaaaaa gattatacag atagttttat tttaaatgct
     3361 ttgaatctac caactatttc agatttagat tcgttatctg atgcttttgc aatcagtaaa
     3421 agacttatct ttttattaac caaaaaaaca gaaaactatt acaaaagatt ttatattaaa
     3481 aagagagatg gtacaagtag agagatactt agtcccacat attctttaaa attaatacaa
     3541 cgatggattt tgaaagaaat attggagaaa gttagtttat cagagcaagc gatggcatat
     3601 aaaaaaggga aaaataatgg cataaagaaa aatgcaatgc atcatagata cagcctatat
     3661 atacttgaaa tggatattaa agattttttc cattcaataa aaagagaaag agtgttttat
     3721 ctttttaaga acttgggcta taacaatatg gtttcaaata tcttaactaa tctatgtact
     3781 tataatgggt atttacctca agggggagtt tgctcgcctt atatttccaa tttaatttgt
     3841 tacaggttag ataatcggtt gtcaggatta tgtggaaaaa gggatattct ttatacaaga
     3901 tatgctgacg atttaacctt ttcaagtgat aataaacaga cacttactaa agcgataaat
     3961 attataatta acataattga ggatgaagga tttaaagtca ataaaaataa gacaagactg
     4021 ctatctccag gttcacacaa aaaagttact ggaattactg tgaatgatag taaaataaag
     4081 gcaagtaagc aattaaaacg aactgttaga gcaatgatac atcaggcaat agttacagcc
     4141 gattatagcc agattgatag aattagaggg atagtatctt atataaattc cattgaagtt
     4201 ggatataggg aaaaagttat aacttacata aatagtttat caaataagga ttacgcattt
     4261 tttgaggaaa tagttgagcg gtttaatgac aacaagattt ttaaggaaat acaagatatg
     4321 aattataaag gatatgatta cgatgaccag gttagtctgg agttagaaaa aatagtgagt
     4381 gccaggcaag aatatctcgg aaaaattgga aaagtggact ttttgtcagc acatatacaa
     4441 agtcaacaag atgagttcaa aacaaatgct gctgcaacta aagaaactta tgaatagtac
     4501 cgttcatatg aggctagagc aggtgatatt attgacaaca tcgttatcct tagatgctac
     4561 tacttttgta taaacgtgca caatatttat ttatatgaaa gtagtgggaa gtggttcgct
     4621 ttccgaggtg ctatgtcagg actatattta atgaagtaag aagtgaagag cgagtgtgag
     4681 ccgttgtagc ttcaacctgt cccttacgaa tagctcttca ccccttactt catcaaaagg
     4741 aataaaggta aggaggacgg tgttgtcaat gatgccacct gcatgatccg cggccgcact
     4801 cgatcgaaat aattttgttt aactttaagg aggtttggaa tgaaccaaat cgtgaagttc
     4861 actgacgact ctggcctggc ggttcaagtt actccagacg atgttcgccg ttatatctgt
     4921 gagaacgcta ctgaaaaaga ggtgggcctc tttctgcaac tctgtcagac tcaacgtctg
     4981 aatccgtttg tgaaagacgc ttacctggtg aaatacggcg gtgctccagc ttctatgatt
     5041 acttcctatc aagtttttaa ccgtcgcgcg tgtcgtgatg ctaactatga tggtatcaaa
     5101 tctggtgtgg ttgttctgcg tgacggtgat gttgtgcata aacgtggtgc tgcgtgctac
     5161 aaaaaggcgg gtgaggagct catcggtggt tgggcggaag ttcgctttaa ggatggccgc
     5221 gagactgcgt atgctgaggt ggcgctcgac gactattcca ccggcaaatc taattgggcg
     5281 aaaatgccgg gtgttatgat cgaaaaatgc gcgaaggctg ctgcttggcg cctcgcgttc
     5341 ccggacactt ttcagggcat gtacgctgcg gaggaaatgg atcaagcgca acagccagaa
     5401 caggtgcgcg ctcaggcgga gcaaccagtg gatctccagc caatccgcga actcttcaag
     5461 ccatattgcg aacacttcgg catcactccg gctgagggta tgactgctgt ttgtggtgcg
     5521 gtgggcgctg aaggcatgca ctctatgacc gagcagcaag ctcgccgtgc tcgcgcttgg
     5581 atggaggaag aaatggctgc gccagctgtg gaagcggagt atgaggttgt tgacgagggc
     5641 gaggtgtttt aaggatccag gagaatacac atggccagca gaggcgtaaa caaggttatt
     5701 ctcgttggta atctgggtca ggacccggaa gtacgctaca tgccaaatgg tggcgcagtt
     5761 gccaacatta cgctggctac ttccgaatcc tggcgtgata aagcgaccgg cgagatgaaa
     5821 gagcagactg aatggcaccg cgttgtgctg ttcggcaaac tggcagaagt ggccagcgaa
     5881 tatctgcgta aaggttctca ggtttatatc gaaggtcagc tgcgtacccg taaatggacc
     5941 gatcaatccg gtcaggatcg ctacaccaca gaagtcgtgg tgaacgttgg cggcaccatg
     6001 cagatgctgg gtggtcgtca gggtggtggc gctccggcag gtggtaatat cggtggtggt
     6061 cagccgcagg gcggttgggg tcagcctcag cagccgcagg gtggcaatca gttcagcggc
     6121 ggcgcgcagt ctcgcccgca gcagtccgct ccggcagcgc cgtctaacga gccgccgatg
     6181 gactttgatg atgacattcc gttctgatct agagtcgacc tgcaggcatg caagcttgcg
     6241 gccgcgtcgt gactgggaaa accctggcga ctagtcttgg actcctgttg atagatccag
     6301 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     6361 ttattggtga gaatccaggg gtccccaata attacgattt aaatttgtgt ctcaaaatct
     6421 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     6481 cataaacagt aatacaaggg gtgttatgag ccatattcag cgtgaaacga gctgtagccg
     6541 tccgcgtctg aacagcaaca tggatgcgga tctgtatggc tataaatggg cgcgtgataa
     6601 cgtgggtcag agcggcgcga ccatttatcg tctgtatggc aaaccggatg cgccggaact
     6661 gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg accgatgaaa tggtgcgtct
     6721 gaactggctg accgaattta tgccgctgcc gaccattaaa cattttattc gcaccccgga
     6781 tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc gcgtttcagg tgctggaaga
     6841 atatccggat agcggcgaaa acattgtgga tgcgctggcc gtgtttctgc gtcgtctgca
     6901 tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt gtgtttcgtc tggcccaggc
     6961 gcagagccgt atgaacaacg gcctggtgga tgcgagcgat tttgatgatg aacgtaacgg
     7021 ctggccggtg gaacaggtgt ggaaagaaat gcataaactg ctgccgttta gcccggatag
     7081 cgtggtgacc cacggcgatt ttagcctgga taacctgatt ttcgatgaag gcaaactgat
     7141 tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt tatcaggatc tggccattct
     7201 gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa cgtctgtttc agaaatatgg
     7261 cattgataat ccggatatga acaaactgca atttcatctg atgctggatg aatttttcta
     7321 ataattaatt ggaccgcggt ccgcgcgttg tccttttccg ctgcataacc ctgcttcggg
     7381 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     7441 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     7501 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     7561 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggcctcag cctgccgcct
     7621 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
     7681 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
     7741 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
     7801 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
     7861 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
     7921 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
     7981 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
     8041 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     8101 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
     8161 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     8221 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     8281 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     8341 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     8401 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     8461 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     8521 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     8581 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     8641 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     8701 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     8761 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     8821 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     8881 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     8941 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     9001 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     9061 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     9121 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
     9181 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
     9241 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
     9301 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
     9361 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
     9421 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
     9481 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
     9541 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
     9601 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
     9661 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
     9721 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
     9781 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
     9841 ggtgccggtg cccgtgccag ctaggcccgc gcaagctgga cgctgggcag acccatgacc
     9901 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
     9961 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
    10021 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
    10081 gc
//
