LOCUS       sequence-482372-       10283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Tsp1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Tsp1
FEATURES             Location/Qualifiers
     source          1..10283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      247..341
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(462..481)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             473..1288
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1455..1563
                     /note="incP origin of transfer"
     CDS             complement(1574..2425)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2412..3251)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3762..4733)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4840..5234
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5272..5358)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5383..6348)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        7248..7328
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             9076..9084
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ccggcaggtg gtaatatcgg tggtggtcag ccgcagggcg gttggggtca gcctcagcag
       61 ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca gtccgctccg
      121 gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt ctgatctaga
      181 gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta
      241 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
      301 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      361 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      421 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      481 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      541 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      601 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      661 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      721 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      781 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      841 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      901 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      961 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
     1021 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
     1081 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
     1141 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
     1201 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1261 tcatctgatg ctggatgaat ttttctaata attaattgga ccgcggtccg cgcgttgtcc
     1321 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     1381 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     1441 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     1501 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     1561 ccgtaggccg gcctcagcct gccgccttgg gccgggtgat gtcgtacttg cccgccgcga
     1621 actcggttac cgtccagccc agcgcgacca gctccggcaa cgcctcgcgc acccgctggc
     1681 ggcgcttgcg catggtcgaa ccactggcct ctgacggcca gacatagccg cacaaggtat
     1741 ctatggaagc cttgccggtt ttgccggggt cgatccagcc acacagccgc tggtgcagca
     1801 ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat gctgatgcgc acatgctggc
     1861 cgccacccat gacggcctgc gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg
     1921 cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg ccgcttgcgg ccattctggg
     1981 cgatgatgga taccttccaa aggcgctcga tgcagtcctg tatgtgcttg agcgccccac
     2041 cactatcgac ctctgccccg atttcctttg ccagcgcccg atagctacct ttgaccacca
     2101 tggcatcagc ggtgacggcc tcccacttgg gttccaggaa cagccggagc tgccgtccgc
     2161 cttcggtctt gggttccggg ccaagcacta ggccattagg cccagccatg gccaccagcc
     2221 cttgcaggat gcgcagatca tcagcgccca gcggctccgg gccgctgaac tcgatccgct
     2281 tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga
     2341 gggcacggaa caggccgggg gccagacagt gcgccgggtc gtgccggacg tggctgaggc
     2401 tgtgcttgtt cttaggcttc accacggggc acccccttgc tcttgcgctg cctctccagc
     2461 acggcgggct tgagcacccc gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg
     2521 ccatagttgg ccttgctcac accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca
     2581 ccccattcct cggcctcggc gctggtcatg ctcgacaggt aggactgcca gcggatgtta
     2641 tcgaccagta ccgagctgcc ccggctggcc tgctgctggt cgcctgcgcc catcatggcc
     2701 gcgcccttgc tggcatggtg caggaacacg atagagcacc cggtatcggc ggcgatggcc
     2761 tccatgcgac cgatgacctg ggccatgggg ccgctggcgt tttcttcctc gatgtggaac
     2821 cggcgcagcg tgtccagcac catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg
     2881 aaccactccg gggccatgat gttgggcagg ctgccgatca gcggctggat cagcaggccg
     2941 tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc caagggcgtg caggcggtga
     3001 tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca ccgggccggt gggcagttcg
     3061 cccacctcca gcagatccgg cccgcctgca atctgtgcgg ccagttgcag ggccagcatg
     3121 gatttaccgg caccaccggg cgacaccagc gccccgaccg taccggccac catgttgggc
     3181 aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct ccagaatatt gataggctta
     3241 tgggtagcca ttgattgcct cctttgcagg cagttggtgg ttaggcgctg gcggggtcac
     3301 tacccccgcc ctgcgccgct ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt
     3361 cgtcggtcag ccagaacttg cgctgacgca tccctttggc cttcatgcgc tcggcatatc
     3421 gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc ggtctgcttg tccttttggt
     3481 ctttcatatc agtcaccgag aaacttgccg gggccgaaag gcttgtcttc gcggaacaag
     3541 gacaaggtgc agccgtcaag gttaaggctg gccatatcag cgactgaaaa gcggccagcc
     3601 tcggccttgt ttgacgtata accaaagcca ccgggcaacc aatagccctt gtcacttttg
     3661 atcaggtaga ccgaccctga agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt
     3721 gagtactcat agtataacag gcgtgagtac caacgcaagc actacatgct gaaatctggc
     3781 ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc gtgccagctc ggcccgcgca
     3841 agctggacgc tgggcagacc catgaccttg ctgacggtgc gctcgatgta atccgcttcg
     3901 tggccgggct tgcgctctgc cagcgctggg ctggcctcgg ccatggcctt gccgatttcc
     3961 tcggcactgc ggccccggct ggccagcttc tgcgcggcga taaagtcgca cttgctgagg
     4021 tcatcaccga agcgcttgac cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg
     4081 cgccggtggc ggctaagctg ccgctcgggc agttcgaggc tggccagcct gcgggccttc
     4141 tcctgctgcc gctgggcctg ctcgatctgc tggccagcct gctgcaccag cgccgggcca
     4201 gcggtggcgg tcttgccctt ggattcacgc agcagcaccc acggctgata accggcgcgg
     4261 gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc ggccatagtg gcggctgtcg
     4321 gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc gggcaatctg cccccgaagt
     4381 tcaccgcctg cggcgtcggc caccttgacc catgcctgat agttcttcgg gctggtttcc
     4441 actaccaggg caggctcccg gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg
     4501 tcgtccacca gcaccagacc atgccgctcc tgctcggcgg gcctgatata cacgtcattg
     4561 ccctgggcat tcatccgctt gagccatggc gtgttctgga gcacttcggc ggctgaccat
     4621 tcccggttca tcatctggcc ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag
     4681 cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca
     4741 ccaagcgatt cccacacatt atacgagccg gaagcataaa gtgtagctag atccgaagga
     4801 tgagccgggc tgaatgatcg accgagacag gccctgcggg gctgcacacg cgcccccacc
     4861 cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg ctccagcgta tttctgcggg
     4921 gtttggtgtg gggtttagcg ggctttgccc gcctttcccc ctgccgcgca gcggtggggc
     4981 ggtgtgtagc ctagcgcagc gaatagacca gctatccggc ctctggccgg gcatattggg
     5041 caagggcagc agcgccccac aagggcgctg ataaccgcgc ctagtggatt attcttagat
     5101 aatcatggat ggatttttcc aacaccccgc cagcccccgc ccctgctggg tttgcaggtt
     5161 tgggggcgtg acagttattg caggggttcg tgacagttat tgcagggggg cgtgacagtt
     5221 attgcagggg ttcgtgacag ttagggcgcg cccagctgtc tagggcggcg gatttgtcct
     5281 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     5341 agcctttcgt tttatttgat gcctttaatt aaacgttcgt aatcaagcca cttccttttt
     5401 gcattgacgc agggtgtcgg aaggcaactc gccgaacgcg ctcctatagt tttcagcgaa
     5461 gcgtcccaaa tgtaagaagc cgtagtctag ggctatctca gttatactac gcacattggc
     5521 actgggatcg ttcaagcagg cgcggatgct ttcgagcttg cggttgcgga tgtagttctt
     5581 cggcgtggtg ccggcgtgct tctcgaacaa attgtagagc gagcgtggac tcatcatcgc
     5641 cagctccgct aaccgctcaa ggctgatatt ccgtttgaga ttctcctcaa tgaattgaac
     5701 gactcgctcg aaagacgggt tacctttgct gaaaatttca cggctgacat tgctgcccag
     5761 catttcgagc agcttggaag cgatgatccc cgcatagtgc tcttggaccc gaggcatcga
     5821 ctttgtatgt tccgcttcgt cacaaactaa cccgagtaga ttgataaagc catcgagttg
     5881 ctggagattg tgtcgcgcgg cgaaacggat accctccctc ggcttgtgcc aattgttgtc
     5941 actgcacgcc cgatcaagga ccactgaggg caatttaacg ataaatttct cgcaatcttc
     6001 tgaataggtc aggtcggctt ggtcatccgg attgagcagc aatagttcgc ccggcgcaaa
     6061 atagtgctcc tggccatggc cacgccacag gcaatggcct ttgagtatta tttgcagatg
     6121 ataacaggtc tctaatccag gcgagattac cctcacgcta ccgccgtagc tgattcgaca
     6181 cagatcgagg catccgaaga ttctgtggtg cagcctgcct gccgggcgcc cgcccttggg
     6241 caggcgaata gagtgcgtac cgacatactg gttaacataa tcggagactg catagggctc
     6301 ggcgtggacg aagatctgac ttttctcgtt caataagcaa aaatccatag ttcacggttc
     6361 tcttatttta atgtgggctg cttggtgtga tgtagaaagg cgccaagtcg atgaaaatgc
     6421 atctcgacgt gatgcgtata cgggttaccc ccattgccac gttgcgccat cctttttgca
     6481 atcagtgacc acttttccaa gcaaaaataa cgccaagcag aacgaagacg ttctttttaa
     6541 gaagcgagaa caccagaagt tcgtgctgtc ggggcatggg gcgacgaatt ggcggataaa
     6601 ggggatctgc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     6661 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgta attacgtatg
     6721 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     6781 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     6841 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     6901 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     6961 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgcatggg
     7021 aattagcttg atctgaccaa cgaccggtag cggagctatc caacggcggt ataccaggaa
     7081 aacacacagc aggtacatca gaacagtacc atgactgaag aacaaatagt tttttcctga
     7141 tccataaagc agaacggcct gctccatgac aaatctggct ccccaactaa tgccccatgc
     7201 agccagcata accagcataa agtgcagtgt ccggtttgat agggataagt ccagccttgc
     7261 aagaagcgga tacaggagtg caaaaaatgg ctatctctag taaggcctac cccttaggct
     7321 ttatgcaacc tctaggatcc gaattcgaag gagatataca tatgtctgac caaccacgta
     7381 cccgccaaga actatacgat cgcattcgac aagttggcaa agaggaattt atccttgaag
     7441 aaatgatacg ttatggtttc tggtctgcac aaggcgaaat gccgcaagat ccagcagatg
     7501 aaattcgccg cgctggagaa attcgtcggg aactgaacga actgcggcaa gaaagtcgaa
     7561 aattacaaga tgaaaaggaa ttacggaagc gcttattaaa gcagcgttta gaagaatcca
     7621 aacgcaagcg tcaagaaaca aaagaacgcc gcgaacaaga acggcgatcg cgtgcagaag
     7681 cttggaagca gaaacagcag caggatatta tctatcttgg ggaggatgta tctgctgggt
     7741 taaataacac tcaaggcaat gaagaaaggt tgcattccta caatttaccg ctatgcaaca
     7801 acgccgaaca aattgccgct gcgatgggaa tcactatagg taaactccgc tttttggcat
     7861 ttaaccgcaa aacctcgaca gtttcgcact acatccgctt caaaatgccg aaaaagacgg
     7921 ggggagaaag aataatttcc gcaccgatgc cgaatttaaa acaggcacag cactggattt
     7981 tagcgaatat tttggaaaag ctggaacttc acgacgctgc acatggcttt agacgcgatc
     8041 gctctatcgt caccaatgct acaccccatg tcggcgctga tgtaattatc aacttcgatt
     8101 taaagaactt tttcccctcc atttcttata aacgagtcaa aggtttattt cactccttcg
     8161 gctattccga agcagcggcg acaattttcg gcttggtgtc taccgctgct gaagtcgaaa
     8221 ctttagaaat tgacggcaaa acttattatg ttgcagtcga tgaacgtcac cttccccaag
     8281 gttcccccgc aagtccagca attactaact tactctgtcg ccgcttagac cgacgtttaa
     8341 cggcgatggc agaaaaatta ggttttactt acacccgcta cgccgacgat ttaacctttt
     8401 ccgcctctgg cgacagtctg cgccatatat gtaatatact caaaggcaca gaatcaattg
     8461 ttacccacga aggctttacc atcaaccaag aaaaaacccg aattttgcga aaatctcgcc
     8521 agcaagaagt tacgggagtg gtggtaaacg ataaacccaa cgttgacaac aaaacattga
     8581 aacgctttcg cgctaccctg tttcaaattg agaaagatgg tttagaagga aagcactggg
     8641 gcaactctga taatttaatc gctgctattg gtggctttgc caattttgtc gcaatggtaa
     8701 atcccgaaaa aggtgcggag tttcaacagc aagtgcaacg catcaaaaat aagtatgttc
     8761 gcaacggtag aagttagtac cgttcatatg aggctagagc aggtgattca cgcgcatctg
     8821 taagctacgc gaaacaacct tgcaaaccgg acgatggttc gtcgtccttg tgcttagtct
     8881 aaccatgcat cactagcctg ctcaagtgaa gagcgagtgt gagccgttgt agcttcaacc
     8941 tgtcccttac gaatagctct tcacccgagc agtctagtcc tgagaccttt acgaaggggt
     9001 aatttgcact tcgatgcgcg tgagccacct gcatgatccg cggccgcact cgatcgaaat
     9061 aattttgttt aactttaagg aggtttggaa tgaaccaaat cgtgaagttc actgacgact
     9121 ctggcctggc ggttcaagtt actccagacg atgttcgccg ttatatctgt gagaacgcta
     9181 ctgaaaaaga ggtgggcctc tttctgcaac tctgtcagac tcaacgtctg aatccgtttg
     9241 tgaaagacgc ttacctggtg aaatacggcg gtgctccagc ttctatgatt acttcctatc
     9301 aagtttttaa ccgtcgcgcg tgtcgtgatg ctaactatga tggtatcaaa tctggtgtgg
     9361 ttgttctgcg tgacggtgat gttgtgcata aacgtggtgc tgcgtgctac aaaaaggcgg
     9421 gtgaggagct catcggtggt tgggcggaag ttcgctttaa ggatggccgc gagactgcgt
     9481 atgctgaggt ggcgctcgac gactattcca ccggcaaatc taattgggcg aaaatgccgg
     9541 gtgttatgat cgaaaaatgc gcgaaggctg ctgcttggcg cctcgcgttc ccggacactt
     9601 ttcagggcat gtacgctgcg gaggaaatgg atcaagcgca acagccagaa caggtgcgcg
     9661 ctcaggcgga gcaaccagtg gatctccagc caatccgcga actcttcaag ccatattgcg
     9721 aacacttcgg catcactccg gctgagggta tgactgctgt ttgtggtgcg gtgggcgctg
     9781 aaggcatgca ctctatgacc gagcagcaag ctcgccgtgc tcgcgcttgg atggaggaag
     9841 aaatggctgc gccagctgtg gaagcggagt atgaggttgt tgacgagggc gaggtgtttt
     9901 aaggatccag gagaatacac atggccagca gaggcgtaaa caaggttatt ctcgttggta
     9961 atctgggtca ggacccggaa gtacgctaca tgccaaatgg tggcgcagtt gccaacatta
    10021 cgctggctac ttccgaatcc tggcgtgata aagcgaccgg cgagatgaaa gagcagactg
    10081 aatggcaccg cgttgtgctg ttcggcaaac tggcagaagt ggccagcgaa tatctgcgta
    10141 aaggttctca ggtttatatc gaaggtcagc tgcgtacccg taaatggacc gatcaatccg
    10201 gtcaggatcg ctacaccaca gaagtcgtgg tgaacgttgg cggcaccatg cagatgctgg
    10261 gtggtcgtca gggtggtggc gct
//
