LOCUS       sequence-482390-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Csp1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Csp1
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            936..1044
                     /note="incP origin of transfer"
     CDS             complement(1055..1906)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1893..2732)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3243..4214)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4321..4715
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4753..4839)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4884..5966)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5928..5947
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5967..6044)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6250..6269
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    6284..6305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6320..6325,6326..6343,6344..6350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6354..6378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7733..7741
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      9187..9281
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9402..9421)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(9413..9459,1..769)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cagcaacatg gatgcggatc tgtatggcta taaatgggcg cgtgataacg tgggtcagag
       61 cggcgcgacc atttatcgtc tgtatggcaa accggatgcg ccggaactgt ttctgaaaca
      121 tggcaaaggc agcgtggcga acgatgtgac cgatgaaatg gtgcgtctga actggctgac
      181 cgaatttatg ccgctgccga ccattaaaca ttttattcgc accccggatg atgcgtggct
      241 gctgaccacc gcgattccgg gcaaaaccgc gtttcaggtg ctggaagaat atccggatag
      301 cggcgaaaac attgtggatg cgctggccgt gtttctgcgt cgtctgcata gcattccggt
      361 gtgcaactgc ccgtttaaca gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat
      421 gaacaacggc ctggtggatg cgagcgattt tgatgatgaa cgtaacggct ggccggtgga
      481 acaggtgtgg aaagaaatgc ataaactgct gccgtttagc ccggatagcg tggtgaccca
      541 cggcgatttt agcctggata acctgatttt cgatgaaggc aaactgattg gctgcattga
      601 tgtgggccgt gtgggcattg cggatcgtta tcaggatctg gccattctgt ggaactgcct
      661 gggcgaattt agcccgagcc tgcaaaaacg tctgtttcag aaatatggca ttgataatcc
      721 ggatatgaac aaactgcaat ttcatctgat gctggatgaa tttttctaat aattaattgg
      781 accgcggtcc gcgcgttgtc cttttccgct gcataaccct gcttcggggt cattatagcg
      841 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
      901 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
      961 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     1021 aatcctgctc tgcgaggctg gccgtaggcc ggcctcagcc tgccgccttg ggccgggtga
     1081 tgtcgtactt gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc agctccggca
     1141 acgcctcgcg cacccgctgg cggcgcttgc gcatggtcga accactggcc tctgacggcc
     1201 agacatagcc gcacaaggta tctatggaag ccttgccggt tttgccgggg tcgatccagc
     1261 cacacagccg ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc acctcgtcca
     1321 tgctgatgcg cacatgctgg ccgccaccca tgacggcctg cgcgatcaag gggttcaggg
     1381 ccacgtacag gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc cgaaacccct
     1441 gccgcttgcg gccattctgg gcgatgatgg ataccttcca aaggcgctcg atgcagtcct
     1501 gtatgtgctt gagcgcccca ccactatcga cctctgcccc gatttccttt gccagcgccc
     1561 gatagctacc tttgaccacc atggcatcag cggtgacggc ctcccacttg ggttccagga
     1621 acagccggag ctgccgtccg ccttcggtct tgggttccgg gccaagcact aggccattag
     1681 gcccagccat ggccaccagc ccttgcagga tgcgcagatc atcagcgccc agcggctccg
     1741 ggccgctgaa ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc agcttgctgc
     1801 gcttgcgctc gccccgcttg agggcacgga acaggccggg ggccagacag tgcgccgggt
     1861 cgtgccggac gtggctgagg ctgtgcttgt tcttaggctt caccacgggg cacccccttg
     1921 ctcttgcgct gcctctccag cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac
     1981 caccgatcag cgaacggtgc gccatagttg gccttgctca caccgaagcg gacgaagaac
     2041 cggcgctggt cgtcgtccac accccattcc tcggcctcgg cgctggtcat gctcgacagg
     2101 taggactgcc agcggatgtt atcgaccagt accgagctgc cccggctggc ctgctgctgg
     2161 tcgcctgcgc ccatcatggc cgcgcccttg ctggcatggt gcaggaacac gatagagcac
     2221 ccggtatcgg cggcgatggc ctccatgcga ccgatgacct gggccatggg gccgctggcg
     2281 ttttcttcct cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg
     2341 gcggcgcgct tgaggccgtc gaaccactcc ggggccatga tgttgggcag gctgccgatc
     2401 agcggctgga tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct gaggtgcgcc
     2461 ccaagggcgt gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg caggtagatc
     2521 accgggccgg tgggcagttc gcccacctcc agcagatccg gcccgcctgc aatctgtgcg
     2581 gccagttgca gggccagcat ggatttaccg gcaccaccgg gcgacaccag cgccccgacc
     2641 gtaccggcca ccatgttggg caaaacgtag tccagcggtg gcggcgctgc tgcgaacgcc
     2701 tccagaatat tgataggctt atgggtagcc attgattgcc tcctttgcag gcagttggtg
     2761 gttaggcgct ggcggggtca ctacccccgc cctgcgccgc tctgagttct tccaggcact
     2821 cgcgcagcgc ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc atccctttgg
     2881 ccttcatgcg ctcggcatat cgcgcttggc gtacagcgtc agggctggcc agcaggtcgc
     2941 cggtctgctt gtccttttgg tctttcatat cagtcaccga gaaacttgcc ggggccgaaa
     3001 ggcttgtctt cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct ggccatatca
     3061 gcgactgaaa agcggccagc ctcggccttg tttgacgtat aaccaaagcc accgggcaac
     3121 caatagccct tgtcactttt gatcaggtag accgaccctg aagcgctttt ttcgtattcc
     3181 ataaaacccc cttctgtgcg tgagtactca tagtataaca ggcgtgagta ccaacgcaag
     3241 cactacatgc tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc
     3301 cgtgccagct cggcccgcgc aagctggacg ctgggcagac ccatgacctt gctgacggtg
     3361 cgctcgatgt aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg gctggcctcg
     3421 gccatggcct tgccgatttc ctcggcactg cggccccggc tggccagctt ctgcgcggcg
     3481 ataaagtcgc acttgctgag gtcatcaccg aagcgcttga ccagcccggc catctcgctg
     3541 cggtactcgt ccagcgccgt gcgccggtgg cggctaagct gccgctcggg cagttcgagg
     3601 ctggccagcc tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc
     3661 tgctgcacca gcgccgggcc agcggtggcg gtcttgccct tggattcacg cagcagcacc
     3721 cacggctgat aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag
     3781 cggccatagt ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc
     3841 cgggcaatct gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga
     3901 tagttcttcg ggctggtttc cactaccagg gcaggctccc ggccctcggc tttcatgtca
     3961 tccaggtcaa actcgctgag gtcgtccacc agcaccagac catgccgctc ctgctcggcg
     4021 ggcctgatat acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg
     4081 agcacttcgg cggctgacca ttcccggttc atcatctggc cggtggtggc gtccctgacg
     4141 ccgatatcga agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc
     4201 ctgtcgttct tcatcgggcc accaagcgat tcccacacat tatacgagcc ggaagcataa
     4261 agtgtagcta gatccgaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg
     4321 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
     4381 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
     4441 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
     4501 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg
     4561 cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg
     4621 cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta
     4681 ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagggcgc gcccagctgt
     4741 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4801 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat taaacgttcg
     4861 taatcaagcc acttcctttt tgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     4981 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     5041 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     5101 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     5161 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5221 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     5281 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     5341 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     5401 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     5461 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     5521 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     5581 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     5641 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     5701 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     5761 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     5821 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     5881 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     5941 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     6001 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     6061 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     6121 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     6181 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     6241 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcataatgtg agttagctca
     6301 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6361 tgagcggata acaattcccc tctaggatcc gaattcgaag gagatataca tatgtcaaat
     6421 tcgcaacttt ggaaatactg taccaaagaa caatgccagg actttatcct ttcgctggat
     6481 cttgtccctc acagcgccgg tatgagcagg gaaaagcagc ttgcctgtct ttatatgctc
     6541 agcaaccgtg gtgcggagaa ttaccataag gagattctga caaaaaagga tggcgggaca
     6601 aggacgctta tggttccctg cagcctactg aaggccgtgc agcgcaatat cctgcaccat
     6661 gtgctggagc aaaggcccgt atcggactgc gccagggcct atcggaaagg cacggacatc
     6721 ctggccaatg ccgggccgca taccgggaga aagcaggtgc tcaagctgga tatcagccat
     6781 ttttttgaca gtattctctt tcccagggtt atcagcgccg ccttcccgga tacgctgttt
     6841 cctccggccg ccgccgcgct tctggcaaac ctgtgctgct gccgggaacg cctgccccag
     6901 ggctctccgg cttccccggc catctccaat ctggtgatgc gcccctttga tgaatccatg
     6961 aggcgctggt gtgaagcgag acagatcgta tacaccagat actgcgatga catgaccttt
     7021 tccggagatt ttgatcccgc cgctgtttat cataaggtaa aaagctatct ggaggcgatg
     7081 ggattttcct taaatgaaaa gaaaacaaag ctcctgaagg acggcgtacg ccagagtgtg
     7141 acgggaattg tggtcaatga aaagccccag gtctccaggg attaccgtaa aaaactgcgg
     7201 caggaaatct attactgcat gaaatatggc gtaacagggc atcttgaaag gctgagggag
     7261 gagaccggga atgcggtgga ggagcagagg tatatatgtt ctgtccttgg aagaattcag
     7321 tttgtgctgc aggtgaatcc ggaggacagg gaatttgcgg aatactgcag gatatggaaa
     7381 gagcggtttg taaaataata ccgttcatat gaggctagag caggtgataa gccggccggc
     7441 agcatatccg ggaaaatatt ttccggcctg acggggagtc cccgccggga gttagaaaca
     7501 gtgggcatgc agaatactgc acccgtaatc cgctccaaag tacatccgca cacttccgtg
     7561 tgcacaccgc cttcggctgt aagtgaagag cgagtgtgag ccgttgtagc ttcaacctgt
     7621 cccttacgaa tagctcttca cccgcagctt acggtatcgg tgtattgtgc cgtccggctt
     7681 gccacctgca tgatccgcgg ccgcactcga tcgaaataat tttgtttaac tttaaggagg
     7741 tttggaatga accaaatcgt gaagttcact gacgactctg gcctggcggt tcaagttact
     7801 ccagacgatg ttcgccgtta tatctgtgag aacgctactg aaaaagaggt gggcctcttt
     7861 ctgcaactct gtcagactca acgtctgaat ccgtttgtga aagacgctta cctggtgaaa
     7921 tacggcggtg ctccagcttc tatgattact tcctatcaag tttttaaccg tcgcgcgtgt
     7981 cgtgatgcta actatgatgg tatcaaatct ggtgtggttg ttctgcgtga cggtgatgtt
     8041 gtgcataaac gtggtgctgc gtgctacaaa aaggcgggtg aggagctcat cggtggttgg
     8101 gcggaagttc gctttaagga tggccgcgag actgcgtatg ctgaggtggc gctcgacgac
     8161 tattccaccg gcaaatctaa ttgggcgaaa atgccgggtg ttatgatcga aaaatgcgcg
     8221 aaggctgctg cttggcgcct cgcgttcccg gacacttttc agggcatgta cgctgcggag
     8281 gaaatggatc aagcgcaaca gccagaacag gtgcgcgctc aggcggagca accagtggat
     8341 ctccagccaa tccgcgaact cttcaagcca tattgcgaac acttcggcat cactccggct
     8401 gagggtatga ctgctgtttg tggtgcggtg ggcgctgaag gcatgcactc tatgaccgag
     8461 cagcaagctc gccgtgctcg cgcttggatg gaggaagaaa tggctgcgcc agctgtggaa
     8521 gcggagtatg aggttgttga cgagggcgag gtgttttaag gatccaggag aatacacatg
     8581 gccagcagag gcgtaaacaa ggttattctc gttggtaatc tgggtcagga cccggaagta
     8641 cgctacatgc caaatggtgg cgcagttgcc aacattacgc tggctacttc cgaatcctgg
     8701 cgtgataaag cgaccggcga gatgaaagag cagactgaat ggcaccgcgt tgtgctgttc
     8761 ggcaaactgg cagaagtggc cagcgaatat ctgcgtaaag gttctcaggt ttatatcgaa
     8821 ggtcagctgc gtacccgtaa atggaccgat caatccggtc aggatcgcta caccacagaa
     8881 gtcgtggtga acgttggcgg caccatgcag atgctgggtg gtcgtcaggg tggtggcgct
     8941 ccggcaggtg gtaatatcgg tggtggtcag ccgcagggcg gttggggtca gcctcagcag
     9001 ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca gtccgctccg
     9061 gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt ctgatctaga
     9121 gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta
     9181 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     9241 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     9301 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     9361 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     9421 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaa
//
