LOCUS       sequence-482392-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Ebu1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Ebu1
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1470..1478
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    2826..2850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2854..2860,2861..2878,2879..2884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2899..2920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2935..2954)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3160..3237
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3238..4320
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3257..3276)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      4365..4451
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4489..4883
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             4990..5961
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6472..7311
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7298..8149
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8160..8268
                     /note="incP origin of transfer"
     CDS             complement(8435..9250)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9242..9261
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(join(9382..9452,1..24))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttactggatc tatcaacagg agtccaagac tagtcgccag ggttttccca gtcacgacgc
       61 ggccgcaagc ttgcatgcct gcaggtcgac tctagatcag aacggaatgt catcatcaaa
      121 gtccatcggc ggctcgttag acggcgctgc cggagcggac tgctgcgggc gagactgcgc
      181 gccgccgctg aactgattgc caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg
      241 ctgaccacca ccgatattac cacctgccgg agcgccacca ccctgacgac cacccagcat
      301 ctgcatggtg ccgccaacgt tcaccacgac ttctgtggtg tagcgatcct gaccggattg
      361 atcggtccat ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag
      421 atattcgctg gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg
      481 ctctttcatc tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt
      541 ggcaactgcg ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac
      601 gagaataacc ttgtttacgc ctctgctggc catgtgtatt ctcctggatc cttaaaacac
      661 ctcgccctcg tcaacaacct catactccgc ttccacagct ggcgcagcca tttcttcctc
      721 catccaagcg cgagcacggc gagcttgctg ctcggtcata gagtgcatgc cttcagcgcc
      781 caccgcacca caaacagcag tcataccctc agccggagtg atgccgaagt gttcgcaata
      841 tggcttgaag agttcgcgga ttggctggag atccactggt tgctccgcct gagcgcgcac
      901 ctgttctggc tgttgcgctt gatccatttc ctccgcagcg tacatgccct gaaaagtgtc
      961 cgggaacgcg aggcgccaag cagcagcctt cgcgcatttt tcgatcataa cacccggcat
     1021 tttcgcccaa ttagatttgc cggtggaata gtcgtcgagc gccacctcag catacgcagt
     1081 ctcgcggcca tccttaaagc gaacttccgc ccaaccaccg atgagctcct cacccgcctt
     1141 tttgtagcac gcagcaccac gtttatgcac aacatcaccg tcacgcagaa caaccacacc
     1201 agatttgata ccatcatagt tagcatcacg acacgcgcga cggttaaaaa cttgatagga
     1261 agtaatcata gaagctggag caccgccgta tttcaccagg taagcgtctt tcacaaacgg
     1321 attcagacgt tgagtctgac agagttgcag aaagaggccc acctcttttt cagtagcgtt
     1381 ctcacagata taacggcgaa catcgtctgg agtaacttga accgccaggc cagagtcgtc
     1441 agtgaacttc acgatttggt tcattccaaa cctccttaaa gttaaacaaa attatttcga
     1501 tcgagtgcgg ccgcggatca tgcaggtggc gacttgtaga aactactttc tatacctgac
     1561 acctaagttc tctgggtgaa gagctattcg taagggacag gttgagctac aacggctcac
     1621 actcgctctt cacttaaaga accgcgagaa tagcaacgct attcgaagcg actatacgga
     1681 gttatccgta tagtcgtggt taaagtgacc cttaaccaag gagcgactac actccaacgt
     1741 gctagtttct acaagtcatc acctgctcta gcctcatatg aacggtatta cttcatttga
     1801 ttaatgtgtt caatgtaact tctggcctct tggttatctg gctcaacttg taaccaaaaa
     1861 cgaatttttc cttctaaact ttttatgtaa ttagggtctt taattttttc attatcaatg
     1921 tgactaaaaa tcccgtattt tttaatataa tgcatggttt ttctaatttc acgcctggtt
     1981 tttctcggta attgtaactt atctttacta acgtcaattc ccgtcactat tttttgtttt
     2041 gtgttgttca gcagtttcgt tttttcttta tttaaaataa aaccatagct ttcaattatg
     2101 tcagttacat aactaataaa ttttgacgga atcgcttcgc cagaaaaaac catatcatcg
     2161 gcatatcggc tatatttcaa attaaatttt aacgccaatt ttgacagtcg ttcatctaat
     2221 ggataaagta atatattact tagtgaaggg ctagtgatgc cgccttgagg taagcaaccg
     2281 tcaagacaac ataatgatgc tagtgcataa gatacatttt tcgcatatcc cagagaatca
     2341 aataatttaa ttacccaatt tattggtatg ctaggaaaaa aatctttgat gtcaatttta
     2401 agtaagaaat gattattcaa atgctgttct gcattatcta taattgattt tcctttctta
     2461 tatgcaaacg catttttatg agttcgtaaa gaatatagca atttttcatg aatccatgtc
     2521 tgacacttac gtagcgatgg ataaggtgaa gatatttttc gatatcctcc tttctttttc
     2581 ggtattttaa attcgcgata aaacgatttt gttccaaaaa caatgtcgtt gatttcaggg
     2641 tattttattc caataagctt agataagtgt tcaacttcaa agattacggg aatgtttcga
     2701 gactctaaag ttttaatata atcaagataa gtagatataa aattggtatt tatacctttt
     2761 tcaagataaa aatcttccca ttcacgtctc atatgtatat ctccttcgaa ttcggatcct
     2821 agaggggaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2881 taaagcctgg ggtgcctaat gagtgagcta actcacatta tgcggttgct ggcgcctata
     2941 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     3001 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     3061 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     3121 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa
     3181 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     3241 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     3301 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     3361 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     3421 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     3481 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     3541 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     3601 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     3661 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     3721 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     3781 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     3841 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     3901 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     3961 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     4021 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     4081 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     4141 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     4201 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     4261 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4321 gcaaaaagga agtggcttga ttacgaacgt ttaattaaag gcatcaaata aaacgaaagg
     4381 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     4441 gtaggacaaa tccgccgccc tagacagctg ggcgcgccct aactgtcacg aacccctgca
     4501 ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc
     4561 caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga
     4621 ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct
     4681 tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca
     4741 ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa
     4801 accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga
     4861 agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct
     4921 catccttcgg atctagctac actttatgct tccggctcgt ataatgtgtg ggaatcgctt
     4981 ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct caaggccatg
     5041 ggctgtgagc gcttcgatat cggcgtcagg gacgccacca ccggccagat gatgaaccgg
     5101 gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat gaatgcccag
     5161 ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt gctggtggac
     5221 gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc tgccctggta
     5281 gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt
     5341 gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga cccggccagc
     5401 gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga caagcacacc
     5461 acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc
     5521 gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca gcggcagcag
     5581 gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg
     5641 cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg cttcggtgat
     5701 gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc
     5761 gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg caagcccggc
     5821 cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag
     5881 cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat ggacaggggc
     5941 gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata ctatgagtac
     6001 tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc ggtctacctg
     6061 atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc
     6121 gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg
     6181 tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa
     6241 agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc
     6301 gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga
     6361 cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg
     6421 tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc
     6481 cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt
     6541 ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa
     6601 tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg
     6661 ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt
     6721 catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct
     6781 gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg
     6841 ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc
     6901 cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg
     6961 gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc
     7021 gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc
     7081 gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg
     7141 ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat
     7201 ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc
     7261 gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca
     7321 cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc
     7381 cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg
     7441 caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca
     7501 agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga
     7561 aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc
     7621 catggtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag
     7681 tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat
     7741 cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga
     7801 ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg
     7861 cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg
     7921 cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat
     7981 agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg
     8041 ccgccagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga
     8101 gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgag gccggcctac
     8161 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     8221 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     8281 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     8341 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     8401 aaggacaacg cgcggaccgc ggtccaatta attattagaa aaattcatcc agcatcagat
     8461 gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac agacgttttt
     8521 gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat
     8581 ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca
     8641 ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt
     8701 tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc
     8761 tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat
     8821 cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca
     8881 gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt
     8941 tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa
     9001 tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat
     9061 cgttcgccac gctgcctttg ccatgtttca gaaacagttc cggcgcatcc ggtttgccat
     9121 acagacgata aatggtcgcg ccgctctgac ccacgttatc acgcgcccat ttatagccat
     9181 acagatccgc atccatgttg ctgttcagac gcggacggct acagctcgtt tcacgctgaa
     9241 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     9301 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca aatttaaatc
     9361 gtaattattg gggacccctg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     9421 tctgaacaaa tccagatgga gttctgaggt ca
//
