LOCUS       sequence-48240-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM
  TITLE     Truncated and full-length thioredoxin-1 have opposing activating and
            inhibitory properties for human complement with relevance to 
            endothelial surfaces.
  JOURNAL   J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19.
  PUBMED    22430737
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2012-04-15"; volume: "188(8)"; pages: "4103-12. 
            Epub 2012 Mar 19"
COMMENT     Sequence Label: pET16b-Trx
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..66
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             79..90
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(462..480)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      476..523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(698..703,704..720,721..726))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatcatcat
       61 catcacagca gcggccatat cgaaggtcgt catatggtga agcagatcga gagcaagact
      121 gcttttcagg aagccttgga cgctgcaggt gataaacttg tagtagttga cttctcagcc
      181 acgtggtgtg ggccttgcaa aatgatcaag cctttctttc attccctctc tgaaaagtat
      241 tccaacgtga tattccttga agtagatgtg gatgactgtc aggatgttgc ttcagagtgt
      301 gaagtcaaat gcatgccaac attccagttt tttaagaagg gacaaaaggt gggtgaattt
      361 tctggagcca ataaggaaaa gcttgaagcc accattaatg aattagtcta aggatccggc
      421 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      481 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      541 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      601 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      661 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
      721 tgtcaaacat gagaattctt ga
//
