LOCUS       sequence-482409-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Ban1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Ban1
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(328..422)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             1868..1876
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    3236..3260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3264..3270,3271..3288,3289..3294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3309..3330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3345..3364)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3570..3647
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3648..4730
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3667..3686)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      4775..4861
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4899..5293
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5400..6371
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6882..7721
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7708..8559
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8570..8678
                     /note="incP origin of transfer"
     CDS             complement(join(8845..9464,1..196))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcacatcgtt cgccacgctg cctttgccat gtttcagaaa cagttccggc gcatccggtt
       61 tgccatacag acgataaatg gtcgcgccgc tctgacccac gttatcacgc gcccatttat
      121 agccatacag atccgcatcc atgttgctgt tcagacgcgg acggctacag ctcgtttcac
      181 gctgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
      241 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaaatt
      301 taaatcgtaa ttattgggga cccctggatt ctcaccaata aaaaacgccc ggcggcaacc
      361 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
      421 tccaagacta gtcgccaggg ttttcccagt cacgacgcgg ccgcaagctt gcatgcctgc
      481 aggtcgactc tagatcagaa cggaatgtca tcatcaaagt ccatcggcgg ctcgttagac
      541 ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa ctgattgcca
      601 ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc gatattacca
      661 cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc gccaacgttc
      721 accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt acgggtacgc
      781 agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc cacttctgcc
      841 agtttgccga acagcacaac gcggtgccat tcagtctgct ctttcatctc gccggtcgct
      901 ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc accatttggc
      961 atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt gtttacgcct
     1021 ctgctggcca tgtgtattct cctggatcct taaaacacct cgccctcgtc aacaacctca
     1081 tactccgctt ccacagctgg cgcagccatt tcttcctcca tccaagcgcg agcacggcga
     1141 gcttgctgct cggtcataga gtgcatgcct tcagcgccca ccgcaccaca aacagcagtc
     1201 ataccctcag ccggagtgat gccgaagtgt tcgcaatatg gcttgaagag ttcgcggatt
     1261 ggctggagat ccactggttg ctccgcctga gcgcgcacct gttctggctg ttgcgcttga
     1321 tccatttcct ccgcagcgta catgccctga aaagtgtccg ggaacgcgag gcgccaagca
     1381 gcagccttcg cgcatttttc gatcataaca cccggcattt tcgcccaatt agatttgccg
     1441 gtggaatagt cgtcgagcgc cacctcagca tacgcagtct cgcggccatc cttaaagcga
     1501 acttccgccc aaccaccgat gagctcctca cccgcctttt tgtagcacgc agcaccacgt
     1561 ttatgcacaa catcaccgtc acgcagaaca accacaccag atttgatacc atcatagtta
     1621 gcatcacgac acgcgcgacg gttaaaaact tgataggaag taatcataga agctggagca
     1681 ccgccgtatt tcaccaggta agcgtctttc acaaacggat tcagacgttg agtctgacag
     1741 agttgcagaa agaggcccac ctctttttca gtagcgttct cacagatata acggcgaaca
     1801 tcgtctggag taacttgaac cgccaggcca gagtcgtcag tgaacttcac gatttggttc
     1861 attccaaacc tccttaaagt taaacaaaat tatttcgatc gagtgcggcc gcggatcatg
     1921 caggtggcat gtttagtgac tacttcacct ttaaattaag ggtgaagagc tattcgtaag
     1981 ggacaggttg aagctacaac ggctcacact cgctcttcac ttttcttaat ttaaaggtga
     2041 ggatggttct gcaattttgg gcagaatcat cattatggtt ttgaaataag aattaaatac
     2101 atacttactt cgattagcac ttgctaaaag tctagtcacc actaaacata tcacctgctc
     2161 tagcctcata tgaacggtat taatcccaat ctaattcctg aattttcttt atcaactcta
     2221 tacccttgct ttgatcaacc atataaatga atcttgctaa cccaattaag taccctttat
     2281 aattagcaat agtaataagc ccatgatatt tcaaatgttc atttacacca aactttttac
     2341 aataataaat ttctttattc agtcttcgaa tatatttttg tggaatcctt acaccttgtg
     2401 gtgtgataac tagcccagtt acgattttcg tatgcctatc cttcaatagt ctagttttct
     2461 tattattgat aataaaatca tacttactca atacacagga cactatctcc tttattttct
     2521 ttactaattt aagatacccc tttttttgga cacctgaaaa agtaatatca tctgcatatc
     2581 tagaatatcg aatgttatat cgactagcta attgttgaaa ttcttcatca atctcacgaa
     2641 agaaaatatt ggacagcatt ggacttgttg gaaaaccttg taccagctca ttattcacag
     2701 ttgttaataa actaaatgct tcactaactg catctgaata accaattcct atgaatattt
     2761 tcttaatctc tttatattta atagaaggaa aaaaatcttt gatatccgtt gaaaacaccc
     2821 aaaaaggttc ttgatgttga tgaacttcgg cattcgttag aatggattta ctcttcacaa
     2881 agccgtgtgc acaagctgac acttcaattt tatttaggat attcttaagt atccattttt
     2941 gaatcgtctt tagtttataa tttggcttcc atacttctct tggttctcca tttcgtttgc
     3001 tgacataata atgattggta ttgtctatca cgaacttctc caaatcatca tctggtaact
     3061 caaaaagtct caaaatctga ttgatgtcaa agataactgg acaatggtta gaatgtatat
     3121 ctttagcata ttgcataaag aatttttttt cttcctccga taaatcgctt gatatgatgt
     3181 tatgtaaaac ttgcttaatc atatgtatat ctccttcgaa ttcggatcct agaggggaat
     3241 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3301 ggtgcctaat gagtgagcta actcacatta tgcggttgct ggcgcctata tcgccgacat
     3361 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     3421 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     3481 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     3541 gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg
     3601 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
     3661 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     3721 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     3781 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     3841 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     3901 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     3961 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     4021 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     4081 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     4141 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     4201 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     4261 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     4321 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     4381 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     4441 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     4501 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     4561 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     4621 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4681 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcaaaaagga
     4741 agtggcttga ttacgaacgt ttaattaaag gcatcaaata aaacgaaagg ctcagtcgaa
     4801 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4861 tccgccgccc tagacagctg ggcgcgccct aactgtcacg aacccctgca ataactgtca
     4921 cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca
     4981 aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga
     5041 ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat
     5101 gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc
     5161 gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga
     5221 aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg
     5281 cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttcgg
     5341 atctagctac actttatgct tccggctcgt ataatgtgtg ggaatcgctt ggtggcccga
     5401 tgaagaacga caggactttg caggccatag gccgacagct caaggccatg ggctgtgagc
     5461 gcttcgatat cggcgtcagg gacgccacca ccggccagat gatgaaccgg gaatggtcag
     5521 ccgccgaagt gctccagaac acgccatggc tcaagcggat gaatgcccag ggcaatgacg
     5581 tgtatatcag gcccgccgag caggagcggc atggtctggt gctggtggac gacctcagcg
     5641 agtttgacct ggatgacatg aaagccgagg gccgggagcc tgccctggta gtggaaacca
     5701 gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg
     5761 ggcagattgc ccggacgctg gccagcgagt acgacgccga cccggccagc gccgacagcc
     5821 gccactatgg ccgcttggcg ggcttcacca accgcaagga caagcacacc acccgcgccg
     5881 gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg
     5941 cgctggtgca gcaggctggc cagcagatcg agcaggccca gcggcagcag gagaaggccc
     6001 gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc
     6061 tggacgagta ccgcagcgag atggccgggc tggtcaagcg cttcggtgat gacctcagca
     6121 agtgcgactt tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg
     6181 gcaaggccat ggccgaggcc agcccagcgc tggcagagcg caagcccggc cacgaagcgg
     6241 attacatcga gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg
     6301 ccgagctggc acgggcaccg gcaccccgcc agcgaggcat ggacaggggc gggccagatt
     6361 tcagcatgta gtgcttgcgt tggtactcac gcctgttata ctatgagtac tcacgcacag
     6421 aagggggttt tatggaatac gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg
     6481 acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc gaggctggcc
     6541 gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg tccttgttcc
     6601 gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa agaccaaaag
     6661 gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc gcgatatgcc
     6721 gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga cgacgaatac
     6781 gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg tagtgacccc
     6841 gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc cataagccta
     6901 tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca
     6961 tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg
     7021 ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg ggcgaactgc
     7081 ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt catcaccgcc
     7141 tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct gacggcctgc
     7201 tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg ttcgacggcc
     7261 tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc cggttccaca
     7321 tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg gaggccatcg
     7381 ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc gcggccatga
     7441 tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc gataacatcc
     7501 gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg
     7561 acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt
     7621 tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc gtgctggaga
     7681 ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca cagcctcagc
     7741 cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg
     7801 ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg caagcggatc
     7861 gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca agggctggtg
     7921 gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga aggcggacgg
     7981 cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc catggtggtc
     8041 aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag tggtggggcg
     8101 ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat cgcccagaat
     8161 ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga ggcggacggg
     8221 cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg cggccagcat
     8281 gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac
     8341 cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat agataccttg
     8401 tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg
     8461 gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg
     8521 ggcaagtacg acatcacccg gcccaaggcg gcaggctgag gccggcctac ggccagcctc
     8581 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     8641 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     8701 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     8761 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aaggacaacg
     8821 cgcggaccgc ggtccaatta attattagaa aaattcatcc agcatcagat gaaattgcag
     8881 tttgttcata tccggattat caatgccata tttctgaaac agacgttttt gcaggctcgg
     8941 gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat ccgcaatgcc
     9001 cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca ggttatccag
     9061 gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt tatgcatttc
     9121 tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc tcgcatccac
     9181 caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat cgctgttaaa
     9241 cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca gcgcatccac
     9301 aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt tgcccggaat
     9361 cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa tggtcggcag
     9421 cggcataaat tcggtcagcc agttcagacg caccatttca tcgg
//
