LOCUS       sequence-482412-        9611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eas1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Eas1
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     115..134
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(255..349)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             1795..1803
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    3310..3334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3338..3344,3345..3362,3363..3368))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3383..3404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3419..3438)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3644..3721
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3722..4804
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3741..3760)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      4849..4935
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4973..5367
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5474..6445
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6956..7795
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7782..8633
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8644..8752
                     /note="incP origin of transfer"
     CDS             complement(join(8919..9611,1..123))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ataaatggtc gcgccgctct gacccacgtt atcacgcgcc catttatagc catacagatc
       61 cgcatccatg ttgctgttca gacgcggacg gctacagctc gtttcacgct gaatatggct
      121 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
      181 atttttatct tgtgcaatgt aacatcagag attttgagac acaaatttaa atcgtaatta
      241 ttggggaccc ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagactagtc
      361 gccagggttt tcccagtcac gacgcggccg caagcttgca tgcctgcagg tcgactctag
      421 atcagaacgg aatgtcatca tcaaagtcca tcggcggctc gttagacggc gctgccggag
      481 cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc tgcggctgct
      541 gaggctgacc ccaaccgccc tgcggctgac caccaccgat attaccacct gccggagcgc
      601 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
      661 tggtgtagcg atcctgaccg gattgatcgg tccatttacg ggtacgcagc tgaccttcga
      721 tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt ttgccgaaca
      781 gcacaacgcg gtgccattca gtctgctctt tcatctcgcc ggtcgcttta tcacgccagg
      841 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
      901 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
      961 gtattctcct ggatccttaa aacacctcgc cctcgtcaac aacctcatac tccgcttcca
     1021 cagctggcgc agccatttct tcctccatcc aagcgcgagc acggcgagct tgctgctcgg
     1081 tcatagagtg catgccttca gcgcccaccg caccacaaac agcagtcata ccctcagccg
     1141 gagtgatgcc gaagtgttcg caatatggct tgaagagttc gcggattggc tggagatcca
     1201 ctggttgctc cgcctgagcg cgcacctgtt ctggctgttg cgcttgatcc atttcctccg
     1261 cagcgtacat gccctgaaaa gtgtccggga acgcgaggcg ccaagcagca gccttcgcgc
     1321 atttttcgat cataacaccc ggcattttcg cccaattaga tttgccggtg gaatagtcgt
     1381 cgagcgccac ctcagcatac gcagtctcgc ggccatcctt aaagcgaact tccgcccaac
     1441 caccgatgag ctcctcaccc gcctttttgt agcacgcagc accacgttta tgcacaacat
     1501 caccgtcacg cagaacaacc acaccagatt tgataccatc atagttagca tcacgacacg
     1561 cgcgacggtt aaaaacttga taggaagtaa tcatagaagc tggagcaccg ccgtatttca
     1621 ccaggtaagc gtctttcaca aacggattca gacgttgagt ctgacagagt tgcagaaaga
     1681 ggcccacctc tttttcagta gcgttctcac agatataacg gcgaacatcg tctggagtaa
     1741 cttgaaccgc caggccagag tcgtcagtga acttcacgat ttggttcatt ccaaacctcc
     1801 ttaaagttaa acaaaattat ttcgatcgag tgcggccgcg gatcatgcag gtggctgggg
     1861 ttccattgag tcacttactt cacctcacgt aagtgagggg tgaagagcta ttcgtaaggg
     1921 acaggttgaa gctacaacgg ctcacactcg ctcttcactt ctcactctgg cgaaggctac
     1981 tacaaagagc gacggaatcc gtgctcgttg gcatcctgtc aaaccattca aacggaatcg
     2041 taggctgcca atggcagttg atacactata tatgtatcta ccacaactaa gtttcacgac
     2101 ggaaccccaa tcacctgctc tagcctcata tgaacggtat tataaagaaa aatctaccca
     2161 aggtaatgag ttgaattcat tccggaactt attccccatg tttgagtcta tggaataaat
     2221 atatcctatt actcctgata gatagttgta gaatccaata actgaacgaa aaccacgcgt
     2281 tttacaatga gttgcgatac catttttttt tgcataatat agatgcattc taactgaatc
     2341 ccttatttct ttaggtattc ttggtgtgtc attatttaca ttaagcccag taagaacttt
     2401 tctagcccct ggcggaatta gcctcgtttt tttgctgtta tcagaaaaac catatccatg
     2461 aagaatttta gatatctctt ttttgatgtg taatgcgctt tttctacatc ccattggtaa
     2521 agaaaaacat atatcgtcag cataacggct gtaagtagcg ccttcagata ttgcaagcga
     2581 tgttagttgc ttatcgagac cgatacaaac cagattcgat aatgcagggc ttgtgggtgc
     2641 tccttgtggt aagctgccca caaattttga atcataacca gtgattgtat gttcattatt
     2701 agtgttacac cacctctttc cctttctttt gggggttact cgtgtgcaca accttgccat
     2761 ttcgaaggat aagagttttg gatatcctag atttagaaaa acatcaaaaa cttggcgctc
     2821 acttatattg ccaaaaaaat ctttaatatc aattttaatc aaccagcctg aaccgcaatg
     2881 acgttgtgca tgctggatta tttttctttt cgggacataa gcatatgctg cagcgtgagg
     2941 aataattcta ttaagtatat ttttgtttat ccaatactgc acagccataa gtgcaggagt
     3001 aggaatggaa atccaacgtt ttcctccggt ccttttcgaa attgaaaagt ttttatatgg
     3061 gaagtctttt cgttcaaggt cgttacggct tacaaaaccg tgtaagtcag aatacaaagt
     3121 atgagataaa ttggctaaat gtgctagaga aaaaactaca ggaaggtttc tttcattaat
     3181 tgtttcccct tgctcgacta agcgtgtcaa atagtgagaa tcatatcctt tagataaacc
     3241 ttccttacga aaaaggttgg ggttccattg agtcatatgt atatctcctt cgaattcgga
     3301 tcctagaggg gaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3361 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attatgcggt tgctggcgcc
     3421 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     3481 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     3541 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     3601 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
     3661 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     3721 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     3781 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     3841 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     3901 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     3961 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     4021 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     4081 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     4141 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     4201 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     4261 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     4321 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     4381 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     4441 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     4501 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     4561 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     4621 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     4681 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     4741 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4801 gtgagcaaaa aggaagtggc ttgattacga acgtttaatt aaaggcatca aataaaacga
     4861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     4921 ctgagtagga caaatccgcc gccctagaca gctgggcgcg ccctaactgt cacgaacccc
     4981 tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg
     5041 cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc
     5101 atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg
     5161 cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta
     5221 cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca
     5281 ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac
     5341 ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc
     5401 ggctcatcct tcggatctag ctacacttta tgcttccggc tcgtataatg tgtgggaatc
     5461 gcttggtggc ccgatgaaga acgacaggac tttgcaggcc ataggccgac agctcaaggc
     5521 catgggctgt gagcgcttcg atatcggcgt cagggacgcc accaccggcc agatgatgaa
     5581 ccgggaatgg tcagccgccg aagtgctcca gaacacgcca tggctcaagc ggatgaatgc
     5641 ccagggcaat gacgtgtata tcaggcccgc cgagcaggag cggcatggtc tggtgctggt
     5701 ggacgacctc agcgagtttg acctggatga catgaaagcc gagggccggg agcctgccct
     5761 ggtagtggaa accagcccga agaactatca ggcatgggtc aaggtggccg acgccgcagg
     5821 cggtgaactt cgggggcaga ttgcccggac gctggccagc gagtacgacg ccgacccggc
     5881 cagcgccgac agccgccact atggccgctt ggcgggcttc accaaccgca aggacaagca
     5941 caccacccgc gccggttatc agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc
     6001 caccgctggc ccggcgctgg tgcagcaggc tggccagcag atcgagcagg cccagcggca
     6061 gcaggagaag gcccgcaggc tggccagcct cgaactgccc gagcggcagc ttagccgcca
     6121 ccggcgcacg gcgctggacg agtaccgcag cgagatggcc gggctggtca agcgcttcgg
     6181 tgatgacctc agcaagtgcg actttatcgc cgcgcagaag ctggccagcc ggggccgcag
     6241 tgccgaggaa atcggcaagg ccatggccga ggccagccca gcgctggcag agcgcaagcc
     6301 cggccacgaa gcggattaca tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt
     6361 ccagcttgcg cgggccgagc tggcacgggc accggcaccc cgccagcgag gcatggacag
     6421 gggcgggcca gatttcagca tgtagtgctt gcgttggtac tcacgcctgt tatactatga
     6481 gtactcacgc acagaagggg gttttatgga atacgaaaaa agcgcttcag ggtcggtcta
     6541 cctgatcaaa agtgacaagg gctattggtt gcccggtggc tttggttata cgtcaaacaa
     6601 ggccgaggct ggccgctttt cagtcgctga tatggccagc cttaaccttg acggctgcac
     6661 cttgtccttg ttccgcgaag acaagccttt cggccccggc aagtttctcg gtgactgata
     6721 tgaaagacca aaaggacaag cagaccggcg acctgctggc cagccctgac gctgtacgcc
     6781 aagcgcgata tgccgagcgc atgaaggcca aagggatgcg tcagcgcaag ttctggctga
     6841 ccgacgacga atacgaggcg ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg
     6901 ggggtagtga ccccgccagc gcctaaccac caactgcctg caaaggaggc aatcaatggc
     6961 tacccataag cctatcaata ttctggaggc gttcgcagca gcgccgccac cgctggacta
     7021 cgttttgccc aacatggtgg ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg
     7081 taaatccatg ctggccctgc aactggccgc acagattgca ggcgggccgg atctgctgga
     7141 ggtgggcgaa ctgcccaccg gcccggtgat ctacctgccc gccgaagacc cgcccaccgc
     7201 cattcatcac cgcctgcacg cccttggggc gcacctcagc gccgaggaac ggcaagccgt
     7261 ggctgacggc ctgctgatcc agccgctgat cggcagcctg cccaacatca tggccccgga
     7321 gtggttcgac ggcctcaagc gcgccgccga gggccgccgc ctgatggtgc tggacacgct
     7381 gcgccggttc cacatcgagg aagaaaacgc cagcggcccc atggcccagg tcatcggtcg
     7441 catggaggcc atcgccgccg ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa
     7501 gggcgcggcc atgatgggcg caggcgacca gcagcaggcc agccggggca gctcggtact
     7561 ggtcgataac atccgctggc agtcctacct gtcgagcatg accagcgccg aggccgagga
     7621 atggggtgtg gacgacgacc agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa
     7681 ctatggcgca ccgttcgctg atcggtggtt caggcggcat gacggcgggg tgctcaagcc
     7741 cgccgtgctg gagaggcagc gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca
     7801 agcacagcct cagccacgtc cggcacgacc cggcgcactg tctggccccc ggcctgttcc
     7861 gtgccctcaa gcggggcgag cgcaagcgca gcaagctgga cgtgacgtat gactacggcg
     7921 acggcaagcg gatcgagttc agcggcccgg agccgctggg cgctgatgat ctgcgcatcc
     7981 tgcaagggct ggtggccatg gctgggccta atggcctagt gcttggcccg gaacccaaga
     8041 ccgaaggcgg acggcagctc cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg
     8101 atgccatggt ggtcaaaggt agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg
     8161 atagtggtgg ggcgctcaag cacatacagg actgcatcga gcgcctttgg aaggtatcca
     8221 tcatcgccca gaatggccgc aagcggcagg ggtttcggct gctgtcggag tacgccagcg
     8281 acgaggcgga cgggcgcctg tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg
     8341 gtggcggcca gcatgtgcgc atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg
     8401 cccgcctgct gcaccagcgg ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt
     8461 ccatagatac cttgtgcggc tatgtctggc cgtcagaggc cagtggttcg accatgcgca
     8521 agcgccgcca gcgggtgcgc gaggcgttgc cggagctggt cgcgctgggc tggacggtaa
     8581 ccgagttcgc ggcgggcaag tacgacatca cccggcccaa ggcggcaggc tgaggccggc
     8641 ctacggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     8701 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
     8761 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
     8821 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
     8881 ggaaaaggac aacgcgcgga ccgcggtcca attaattatt agaaaaattc atccagcatc
     8941 agatgaaatt gcagtttgtt catatccgga ttatcaatgc catatttctg aaacagacgt
     9001 ttttgcaggc tcgggctaaa ttcgcccagg cagttccaca gaatggccag atcctgataa
     9061 cgatccgcaa tgcccacacg gcccacatca atgcagccaa tcagtttgcc ttcatcgaaa
     9121 atcaggttat ccaggctaaa atcgccgtgg gtcaccacgc tatccgggct aaacggcagc
     9181 agtttatgca tttctttcca cacctgttcc accggccagc cgttacgttc atcatcaaaa
     9241 tcgctcgcat ccaccaggcc gttgttcata cggctctgcg cctgggccag acgaaacaca
     9301 cgatcgctgt taaacgggca gttgcacacc ggaatgctat gcagacgacg cagaaacacg
     9361 gccagcgcat ccacaatgtt ttcgccgcta tccggatatt cttccagcac ctgaaacgcg
     9421 gttttgcccg gaatcgcggt ggtcagcagc cacgcatcat ccggggtgcg aataaaatgt
     9481 ttaatggtcg gcagcggcat aaattcggtc agccagttca gacgcaccat ttcatcggtc
     9541 acatcgttcg ccacgctgcc tttgccatgt ttcagaaaca gttccggcgc atccggtttg
     9601 ccatacagac g
//
