LOCUS       sequence-48243-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM
  TITLE     Truncated and full-length thioredoxin-1 have opposing activating and
            inhibitory properties for human complement with relevance to 
            endothelial surfaces.
  JOURNAL   J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19.
  PUBMED    22430737
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2012-04-15"; volume: "188(8)"; pages: "4103-12. 
            Epub 2012 Mar 19"
COMMENT     Sequence Label: pET16b-Trx C35S
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..65
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             78..89
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(461..479)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      475..522
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(697..702,703..719,720..725))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(752..770)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggccatca tcatcatcat catcatcatc
       61 atcacagcag cggccatatc gaaggtcgtc atatggtgaa gcagatcgag agcaagactg
      121 cttttcagga agccttggac gctgcaggtg ataaacttgt agtagttgac ttctcagcca
      181 cgtggtgtgg gcctagcaaa atgatcaagc ctttctttca ttccctctct gaaaagtatt
      241 ccaacgtgat attccttgaa gtagatgtgg atgactgtca ggatgttgct tcagagtgtg
      301 aagtcaaatg catgccaaca ttccagtttt ttaagaaggg acaaaaggtg ggtgaatttt
      361 ctggagccaa taaggaaaag cttgaagcca ccattaatga attagtctaa ggatccggct
      421 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      481 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      541 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
      601 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
      661 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
      721 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      781 aatgtcatga taataatggt ttct
//
