LOCUS       sequence-48244-e       10080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB
  TITLE     Adenoviral expression of CREB protects neurons from apoptotic and 
            excitotoxic stress.
  JOURNAL   Neuroreport. 2004 May 19;15(7):1171-5.
  PUBMED    15129168
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroreport."; date: "2004-05-19"; volume: "15(7)"; pages: "1171-5"
COMMENT     Sequence Label: pXCX.CMV.ACREB.WPRE
FEATURES             Location/Qualifiers
     source          1..10080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..107
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     195..345
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    470..694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     683..700
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    727..1315
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             833..844
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1242..1262
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1364..2077)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1404..1424)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2019..2047
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      2074..2083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2122..2673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2124..2696
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2283..2302)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2512..2529
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(2897..2929)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(2933..2956)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2980..2998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3041..3060)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          3043..3175
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(3233..3251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3297..3500)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3326..3346)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3501..3880
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             7636..7827
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7821..7843)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7929..8069
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7984..8003
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8084..8101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8255..8843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8335..8354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9014..9805,9806..9874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9637..9656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9875..9979)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10047..10065
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattcatcat caataatata ccttattttg gattgaagcc aatatgataa tgagggggtg
       61 gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag tgtggcggaa
      121 gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa aagtgacgtt
      181 tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta ggcggatgtt
      241 gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa actgaataag
      301 aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaatatttg tctagggccg
      361 cggggacttt gaccgtttac gtggagactc gcccaggtgt ttttctcagg tgttttccgc
      421 gttccgggtc aaagttggcg ttttattatt atagtcagtc tagctggttc catagagccc
      481 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
      541 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
      601 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
      661 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcgtac cgagctcgaa
      721 ttccaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
      781 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
      841 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
      901 gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc
      961 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     1021 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     1081 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     1141 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     1201 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     1261 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga
     1321 cggatcctct agatgcatgc tcgagcggcc gccctcgagt ttatttgtat agttcatcca
     1381 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
     1441 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
     1501 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
     1561 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
     1621 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     1681 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     1741 tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaatatagtt ctttcctgta
     1801 cataaccttc gggcatggca ctcttgaaaa agtcatgccg tttcatatga tctgggtatc
     1861 tcgcaaagca ttgaagacca tacgcaaaag tagtgacaag tgttggccat ggaacaggta
     1921 gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca tcaccttcac
     1981 cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg
     2041 ggacaactcc agtgaaaagt tcttctcctt tacccatggt ggcggcccgg tcgactctag
     2101 ctagaggtac cacgcgtgcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     2161 tggttcgggg ggcctagacg ttttttaacc tcgactaaac acatgtaaag catgtgcacc
     2221 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
     2281 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
     2341 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
     2401 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
     2461 ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt
     2521 tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg
     2581 ccctcacatt gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc
     2641 ggccttattc caagcggctt cggccagtaa cgttaggggg gggggaggga gaggggcgga
     2701 attcttaatc tgacttgtgg cagtaaaggt ccttaagtgc ttttagctcc tcaatcaatg
     2761 ttttgttttg gttttcaagc actgccactc tgttctcgag ttctgccagt tcctgctcca
     2821 gctcttcggc ctctttttcc agctcttcgt tttcacgggc cagttcctca gcacgttgtt
     2881 ccaggtcagg atcccgaccc atttgctgtc caccagtcat gctagccata tgcttgtcat
     2941 cgtcgtcctt gtagtccatg gtggaattct cgaggctagc ctatagtgag tcgtattaag
     3001 tactctagcc ttaagagctg taattgaact gggagtggac acctgtggag agaaaggcaa
     3061 agtggatgtc agtaagacca ataggtgcct atcagaaacg caagagtctt ctctgtctcg
     3121 acaagcccag tttctattgg tctccttaaa cctgtcttgt aaccttgata cttacctgcc
     3181 cagtgcctca cgaccaactt cgatccgagc tcggtaccaa gcttccggtc tccctatagt
     3241 gagtcgtatt aatttcgata agccagttaa gcagtgggtt ctctagttag ccagagagct
     3301 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     3361 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     3421 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     3481 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     3541 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     3601 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     3661 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     3721 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     3781 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     3841 gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt
     3901 acatcgctag angatctgga aggtgctgag gtacgatgag acccgcacca ggtgcagacc
     3961 ctgcgagtgt ggcggtaaac atattaggaa ccagcctgtg atgctggatg tgaccgagga
     4021 gctgaggccc gatcacttgg tgctggcctg cacccgcgct gagtttggct ctagcgatga
     4081 agatacagat tgaggtactg aaatgtgtgg gcgtggctta agggtgggaa agaatatata
     4141 aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg ccatgagcac
     4201 caactcgttt gatggaagca ttgtgagctc atatttgaca acgcgcatgc ccccatgggc
     4261 cggggtgcgt cagaatgtga tgggctccag cattgatggt cgccccgtcc tgcccgcaaa
     4321 ctctactacc ttgacctacg agaccgtgtc tggaacgccg ttggagactg cagcctccgc
     4381 cgccgcttca gccgctgcag ccaccgcccg cgggattgtg actgactttg ctttcctgag
     4441 cccgcttgca agcagtgcag cttcccgttc atccgcccgc gatgacaagt tgacggctct
     4501 tttggcacaa ttggattctt tgacccggga acttaatgtc gtttctcagc agctgttgga
     4561 tctgcgccag caggtttctg ccctgaaggc ttcctcccct cccaatgcgg tttaaaacat
     4621 aaataaaaaa ccagactctg tttggatttg gatcaagcaa gtgtcttgct gtctttattt
     4681 aggggttttg cgcgcgcggt aggcccggga ccagcggtct cggtcgttga gggtcctgtg
     4741 tattttttcc aggacgtggt aaaggtgact ctggatgttc agatacatgg gcataagccc
     4801 gtctctgggg tggaggtagc accactgcag agcttcatgc tgcggggtgg tgttgtagat
     4861 gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa atgtctttca gtagcaagct
     4921 gattgccagg ggcaggccct tggtgtaagt gtttacaaag cggttaagct gggatgggtg
     4981 catacgtggg gatatgagat gcatcttgga ctgtattttt aggttggcta tgttcccagc
     5041 catatccctc cggggattca tgttgtgcag aaccaccagc acagtgtatc cggtgcactt
     5101 gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag aacttggaga cgcccttgtg
     5161 acctccaaga ttttccatgc attcgtccat aatgatggca atgggcccac gggcggcggc
     5221 ctgggcgaag atatttctgg gatcactaac gtcatagttg tgttccagga tgagatcgtc
     5281 ataggccatt tttacaaagc gcgggcggag ggtgccagac tgcggtataa tggttccatc
     5341 cggcccaggg gcgtagttac cctcacagat ttgcatttcc cacgctttga gttcagatgg
     5401 ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt tccggggtag gggagatcag
     5461 ctgggaagaa agcaggttcc tgagcagctg cgacttaccg cagccggtgg gcccgtaaat
     5521 cacacctatt accgggtgca actggtagtt aagagagctg cagctgccgt catccctgag
     5581 caggggggcc acttcgttaa gcatgtccct gactcgcatg ttttccctga ccaaatccgc
     5641 cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag gaagcaaagt ttttcaacgg
     5701 tttgagaccg tccgccgtag gcatgctttt gagcgtttga ccaagcagtt ccaggcggtc
     5761 ccacagctcg gtcacctgct ctacggcatc tcgatccagc atatctcctc gtttcgcggg
     5821 ttggggcggc tttcgctgta cggcagtagt cggtgctcgt ccagacgggc cagggtcatg
     5881 tctttccacg ggcgcagggt cctcgtcagc gtagtctggg tcacggtgaa ggggtgcgct
     5941 ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc tgctggtgct gaagcgctgc
     6001 cggtcttcgc cctgcgcgtc ggccaggtag catttgacca tggtgtcata gtccagcccc
     6061 tccgcggcgt ggcccttggc gcgcagcttg cccttggagg aggcgccgca cgaggggcag
     6121 tgcagacttt tgagggcgta gagcttgggc gcgagaaata ccgattccgg ggagtaggca
     6181 tccgcgccgc aggccccgca gacggtctcg cattccacga gccaggtgag ctctggccgt
     6241 tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc gtttcttacc tctggtttcc
     6301 atgagccggt gtccacgctc ggtgacgaaa aggctgtccg tgtccccgta tacagacttg
     6361 agaggcctgt cctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     6421 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     6481 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     6541 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     6601 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     6661 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     6721 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     6781 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     6841 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     6901 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     6961 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     7021 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     7081 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     7141 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
     7201 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     7261 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     7321 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     7381 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     7441 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     7501 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     7561 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     7621 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     7681 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     7741 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     7801 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7861 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7921 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7981 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     8041 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9301 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     9361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9601 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10021 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
//
