LOCUS       sequence-482449-        3750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Gong L, Luo J, Yang E, Ru B, Qi Z, Yang Y, Rani A, Purohit A, Zhang 
            Y, Guan G, Paul R, Vu T, Chen Z, Ji R, Day CP, Wu C, Merlino G, 
            Fitzgerald D, Altan-Bonnet G, Aldape K, Wu J, Guan X, Jiang P
  TITLE     Cancer immunology data engine reveals secreted AOAH as a potential 
            immunotherapy.
  JOURNAL   Cell. 2025 Jul 24:S0092-8674(25)00793-7. doi: 
            10.1016/j.cell.2025.07.004.
  PUBMED    40730154
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Jul 24:S0092-8674(25)00793-7. doi: 10.1016/j.cell.2025.07.004."
COMMENT     Sequence Label: PKT2/CLP-Vector
FEATURES             Location/Qualifiers
     source          1..3750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(1071..1089)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1191..1312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1228..1247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1282..1301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1455..1481)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     2429..2448
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2429..2447
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2789..2808)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2800..3609
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2867..2886)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg gttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa cagatttaaa
      781 tggtaccaga tccctataca gttgaagtcg gaagtttaca tacaccttag ccaaatacat
      841 ttaaactcac tttttcacaa ttcctgacat ttaatcctag taaaaattcc ctgtcttagg
      901 tcagttagga tcaccacttt attttaagaa tgtgaaatat cagaataata gtagagagaa
      961 tgattcattt cagcttttat ttctttcatc acattcccag tgggtcagaa gtttacatac
     1021 actcaattag tatttggtag cattgccttt aaattgttta acttggtctc cctttagtga
     1081 gggttaattg ataattccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1141 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     1321 ggccagctag ctgggccctg gtacctagat ctggatgcat tcgcgcgcac gtacggtctc
     1381 gaggaattcc gacagcgtgg cttctctcaa atgcacccag aaataaaaac cagtccaccg
     1441 ccgcccatat gcgaagcgta atctggaaca tcgtatgggt aagcagcgtc gtcctcggga
     1501 ttcgacgagc aaacgcagcc catggtggaa gctactgtac accaacctgt caggagagga
     1561 aagagaagaa ggttagtaca attgtctaga tgcattccta gggctgcagg gttcatagtg
     1621 ccacttttcc tgcactgccc catctcctgc ccaccctttc ccaggcatag acagtcagtg
     1681 acttaccaaa ctcacaggag ggagaaggca gaagcttgaa tgttcacaga gactactgca
     1741 cttatatatg gttctccccc accctgggga aaaaggtgga gccagtacac cacatcactt
     1801 tcccagttta cccaagcccc accttctcta ggcaccagtt caattgccca cccctccccc
     1861 caacttctca gggactgtgg gccatgtgct ctctgcccac tgaggggcac tcagccctca
     1921 agcatgctct tctccactag tcacccctat tgaccttatg tatgtgccaa taatgggaaa
     1981 aacccattga ctcaccccct attgaccttt tgtactgggc aaaacccaat ggaaagtccc
     2041 tattgactca gtgtacttgg ctccaatggg actttcctgt tgattcaccc ctattgacct
     2101 tatgtactgg gcaaaaccca ttggaaagtc cctaatgact cagtatacat gtcagtaatg
     2161 ggaaaaaccc attggcttac ctcccattga ccttatgtac tgggcaaaac ccattggaaa
     2221 gtccctattg actcaatgta cttggctcca atgggacttt cctgttgact caccccctat
     2281 tgaccttatg tactgggcaa aacccaatgg aaagtcccta ttgagtcagt gtacttggct
     2341 ccaatgggtt tttcccattg actcctgcag gaattatcgc tagcttgacc ggtatgcatc
     2401 ctaggtttaa actagtaatc ccgggatcta atacgactca ctatagggag accatcattt
     2461 tctggaattt tccaagctgt ttaaaggcac agtcaactta gtgtatgtaa acttctgacc
     2521 cactggaatt gtgatacagt gaattataag tgaaataatc tgtctgtaaa caattgttgg
     2581 aaaaatgact tgtgtcatgc acaaagtaga tgtcctaact gacttgccaa aactattgtt
     2641 tgttaacaag aaatttgtgg agtagttgaa aaacgagttt taatgactcc aacttaagtg
     2701 tatgtaaact tccgacttca actgtatagg gatctggatc cagctgcatc atgaacaata
     2761 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2821 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2881 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2941 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3001 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3061 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     3121 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3181 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3241 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3301 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     3361 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3421 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3481 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3541 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3601 tttttctaat cagaattggt taattggttg taacactggc aaaaaggatc taggtgaaga
     3661 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3721 cagaccccgt agaaaagatc aaaggatctt
//
