LOCUS       sequence-48246-e        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Glover CP, Bienemann AS, Heywood DJ, Cosgrave AS, Uney JB
  TITLE     Adenoviral-mediated, high-level, cell-specific transgene expression:
            a SYN1-WPRE cassette mediates increased transgene expression with no
            loss of neuron specificity.
  JOURNAL   Mol Ther. 2002 May;5(5 Pt 1):509-16.
  PUBMED    11991741
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.";
            date: "2002-05"; volume: "5(5 Pt 1)"; pages: "509-16"
COMMENT     Sequence Label: pXCX.CMV.EGFP
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..107
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     195..345
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    470..694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     683..700
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(740..1453,1454..1456,1457..1459))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(743..1453,1454..1456,1457..1459))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(785..806)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1134..1153
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1393..1414
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1456..1465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1506..1525)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1507..1525)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1570..1773)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1599..1619)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1774..2153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             5909..6100
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6094..6116)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6202..6342
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6257..6276
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6357..6374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6528..7116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6608..6627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7287..8078,8079..8147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7910..7929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8148..8252)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8320..8338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattcatcat caataatata ccttattttg gattgaagcc aatatgataa tgagggggtg
       61 gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag tgtggcggaa
      121 gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa aagtgacgtt
      181 tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta ggcggatgtt
      241 gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa actgaataag
      301 aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaatatttg tctagggccg
      361 cggggacttt gaccgtttac gtggagactc gcccaggtgt ttttctcagg tgttttccgc
      421 gttccgggtc aaagttggcg ttttattatt atagtcagtc tagctggttc catagagccc
      481 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
      541 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
      601 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
      661 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagat
      721 gcatgctcga gcggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
      781 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      841 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      901 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      961 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     1021 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     1081 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     1141 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     1201 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     1261 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     1321 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     1381 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     1441 cctcgccctt gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accaagcttg
     1501 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
     1561 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     1621 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     1681 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     1741 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     1801 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     1861 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     1921 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     1981 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2041 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     2101 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     2161 atatctggcc cgtacatcgc tagangatct ggaaggtgct gaggtacgat gagacccgca
     2221 ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct gtgatgctgg
     2281 atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc gctgagtttg
     2341 gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc ttaagggtgg
     2401 gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca gcagccgccg
     2461 ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg acaacgcgca
     2521 tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat ggtcgccccg
     2581 tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg ccgttggaga
     2641 ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact
     2701 ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca
     2761 agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc
     2821 agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg
     2881 cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt
     2941 gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt
     3001 tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca
     3061 tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg
     3121 tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt
     3181 tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa
     3241 gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg
     3301 ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt
     3361 atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg
     3421 agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc
     3481 cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca
     3541 ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta
     3601 taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt
     3661 tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg
     3721 taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg
     3781 tgggcccgta aatcacacct attaccgggt gcaactggta gttaagagag ctgcagctgc
     3841 cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc
     3901 tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa
     3961 agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca
     4021 gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc
     4081 ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg
     4141 ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt
     4201 gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt
     4261 gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc
     4321 atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc
     4381 gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc
     4441 cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt
     4501 gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt
     4561 acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc
     4621 gtatacagac ttgagaggcc tgtcctcgac cgatgccctt gagagccttc aacccagtca
     4681 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4741 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     4801 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     4861 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     4921 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     4981 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     5041 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     5101 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
     5161 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     5221 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     5281 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     5341 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     5401 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
     5461 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
     5521 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
     5581 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
     5641 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
     5701 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
     5761 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
     5821 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
     5881 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
     5941 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     6001 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     6061 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6121 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6181 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     6241 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6301 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     6361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6961 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     7621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     7921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8281 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8341 tttcgtcttc aag
//
