LOCUS       sequence-48247-e        8190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Glover CP, Bienemann AS, Heywood DJ, Cosgrave AS, Uney JB
  TITLE     Adenoviral-mediated, high-level, cell-specific transgene expression:
            a SYN1-WPRE cassette mediates increased transgene expression with no
            loss of neuron specificity.
  JOURNAL   Mol Ther. 2002 May;5(5 Pt 1):509-16.
  PUBMED    11991741
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.";
            date: "2002-05"; volume: "5(5 Pt 1)"; pages: "509-16"
COMMENT     Sequence Label: pXCX.Syn.EGFP
FEATURES             Location/Qualifiers
     source          1..8190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(21..468)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4258..4449
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4443..4465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4551..4691
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4606..4625
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4706..4723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4877..5465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4957..4976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5636..6427,6428..6496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6259..6278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6497..6601)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6669..6687
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   6707..6809
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     6897..7047
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    7172..7396
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7385..7402
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(7442..8155,8156..8158,8159..8161))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7445..8155,8156..8158,8159..8161))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7487..7508)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7836..7855
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8095..8116
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8158..8167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtaccaagct ctagagtcga ctgcgctctc aggcacgaca cgactcctcc gctgcccacc
       61 gcagactgag gcagcgctga gtcgccggcg ccgcagcgca gatggtcgcg cccgtgcccc
      121 cctatctcgc gcctcgcgtg gtgcggtccg gctgggccgg cggcggcgcg gacgcgacca
      181 aggtggccgg gaaggggagt ttgcggggga ccggcgagtg acgtcagcgc gccttcagtg
      241 ctgaggcggc ggtggcgcgc gccgccaggc gggggcgaag gcactgtccg cggtgctgaa
      301 gctggcagtg cgcacgcgcc tcgccgcatc ctgtttcccc tccccctctc tgatagggga
      361 tgcgcaattt ggggaatggg ggttgggtgc ttgtccagtg ggtcggggtc ggtcgtcagg
      421 taggcacccc caccccgcct catcctggtc ctaaaaccca cttgcactca tacgcagggc
      481 cctctgcagc tggcgccatc gatacgcgta tatctggccc gtacatcgct agangatctg
      541 gaaggtgctg aggtacgatg agacccgcac caggtgcaga ccctgcgagt gtggcggtaa
      601 acatattagg aaccagcctg tgatgctgga tgtgaccgag gagctgaggc ccgatcactt
      661 ggtgctggcc tgcacccgcg ctgagtttgg ctctagcgat gaagatacag attgaggtac
      721 tgaaatgtgt gggcgtggct taagggtggg aaagaatata taaggtgggg gtcttatgta
      781 gttttgtatc tgttttgcag cagccgccgc cgccatgagc accaactcgt ttgatggaag
      841 cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt
      901 gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta
      961 cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc
     1021 agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc
     1081 agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc
     1141 tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc
     1201 tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc
     1261 tgtttggatt tggatcaagc aagtgtcttg ctgtctttat ttaggggttt tgcgcgcgcg
     1321 gtaggcccgg gaccagcggt ctcggtcgtt gagggtcctg tgtatttttt ccaggacgtg
     1381 gtaaaggtga ctctggatgt tcagatacat gggcataagc ccgtctctgg ggtggaggta
     1441 gcaccactgc agagcttcat gctgcggggt ggtgttgtag atgatccagt cgtagcagga
     1501 gcgctgggcg tggtgcctaa aaatgtcttt cagtagcaag ctgattgcca ggggcaggcc
     1561 cttggtgtaa gtgtttacaa agcggttaag ctgggatggg tgcatacgtg gggatatgag
     1621 atgcatcttg gactgtattt ttaggttggc tatgttccca gccatatccc tccggggatt
     1681 catgttgtgc agaaccacca gcacagtgta tccggtgcac ttgggaaatt tgtcatgtag
     1741 cttagaagga aatgcgtgga agaacttgga gacgcccttg tgacctccaa gattttccat
     1801 gcattcgtcc ataatgatgg caatgggccc acgggcggcg gcctgggcga agatatttct
     1861 gggatcacta acgtcatagt tgtgttccag gatgagatcg tcataggcca tttttacaaa
     1921 gcgcgggcgg agggtgccag actgcggtat aatggttcca tccggcccag gggcgtagtt
     1981 accctcacag atttgcattt cccacgcttt gagttcagat ggggggatca tgtctacctg
     2041 cggggcgatg aagaaaacgg tttccggggt aggggagatc agctgggaag aaagcaggtt
     2101 cctgagcagc tgcgacttac cgcagccggt gggcccgtaa atcacaccta ttaccgggtg
     2161 caactggtag ttaagagagc tgcagctgcc gtcatccctg agcagggggg ccacttcgtt
     2221 aagcatgtcc ctgactcgca tgttttccct gaccaaatcc gccagaaggc gctcgccgcc
     2281 cagcgatagc agttcttgca aggaagcaaa gtttttcaac ggtttgagac cgtccgccgt
     2341 aggcatgctt ttgagcgttt gaccaagcag ttccaggcgg tcccacagct cggtcacctg
     2401 ctctacggca tctcgatcca gcatatctcc tcgtttcgcg ggttggggcg gctttcgctg
     2461 tacggcagta gtcggtgctc gtccagacgg gccagggtca tgtctttcca cgggcgcagg
     2521 gtcctcgtca gcgtagtctg ggtcacggtg aaggggtgcg ctccgggctg cgcgctggcc
     2581 agggtgcgct tgaggctggt cctgctggtg ctgaagcgct gccggtcttc gccctgcgcg
     2641 tcggccaggt agcatttgac catggtgtca tagtccagcc cctccgcggc gtggcccttg
     2701 gcgcgcagct tgcccttgga ggaggcgccg cacgaggggc agtgcagact tttgagggcg
     2761 tagagcttgg gcgcgagaaa taccgattcc ggggagtagg catccgcgcc gcaggccccg
     2821 cagacggtct cgcattccac gagccaggtg agctctggcc gttcggggtc aaaaaccagg
     2881 tttcccccat gctttttgat gcgtttctta cctctggttt ccatgagccg gtgtccacgc
     2941 tcggtgacga aaaggctgtc cgtgtccccg tatacagact tgagaggcct gtcctcgacc
     3001 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     3061 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     3121 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     3181 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     3241 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     3301 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     3361 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     3421 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     3481 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     3541 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     3601 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     3661 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     3721 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     3781 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     3841 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     3901 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     3961 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     4021 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     4081 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     4141 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     4201 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     4261 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     4321 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     4381 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     4441 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4501 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     4561 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4621 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4681 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5281 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5941 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6241 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6601 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     6661 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcatc atcaataata
     6721 taccttattt tggattgaag ccaatatgat aatgaggggg tggagtttgt gacgtggcgc
     6781 ggggcgtggg aacggggcgg gtgacgtagt agtgtggcgg aagtgtgatg ttgcaagtgt
     6841 ggcggaacac atgtaagcga cggatgtggc aaaagtgacg tttttggtgt gcgccggtgt
     6901 acacaggaag tgacaatttt cgcgcggttt taggcggatg ttgtagtaaa tttgggcgta
     6961 accgagtaag atttggccat tttcgcggga aaactgaata agaggaagtg aaatctgaat
     7021 aattttgtgt tactcatagc gcgtaatatt tgtctagggc cgcggggact ttgaccgttt
     7081 acgtggagac tcgcccaggt gtttttctca ggtgttttcc gcgttccggg tcaaagttgg
     7141 cgttttatta ttatagtcag tctagctggt tccatagagc ccaccgcatc cccagcatgc
     7201 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     7261 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     7321 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     7381 caactagaag gcacagtcga ggctgatcag cgagctctag atgcatgctc gagcggccgc
     7441 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     7501 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     7561 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     7621 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     7681 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     7741 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     7801 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     7861 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     7921 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     7981 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     8041 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     8101 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     8161 tggtggcgac cggtggatcc cgggcccgcg
//
