LOCUS       sequence-482478-       10146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eco2 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Eco2
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            247..355
                     /note="incP origin of transfer"
     CDS             complement(366..1217)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1204..2043)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2554..3525)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      3632..4026
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4064..4150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4195..5277)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5239..5258
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5278..5355)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5561..5580
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    5595..5616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5631..5636,5637..5654,5655..5661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5665..5689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7731..7739
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      9185..9279
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9400..9419)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(9411..10146,1..80)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gtctgtttca gaaatatggc attgataatc cggatatgaa caaactgcaa tttcatctga
       61 tgctggatga atttttctaa taattaattg gaccgcggtc cgcgcgttgt ccttttccgc
      121 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
      181 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
      241 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
      301 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc
      361 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
      421 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
      481 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
      541 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
      601 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
      661 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
      721 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
      781 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
      841 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
      901 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
      961 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
     1021 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
     1081 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
     1141 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
     1201 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
     1261 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
     1321 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     1381 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     1441 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     1501 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     1561 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     1621 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     1681 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     1741 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     1801 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     1861 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     1921 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     1981 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     2041 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     2101 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     2161 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     2221 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     2281 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     2341 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     2401 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     2461 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     2521 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     2581 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     2641 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     2701 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     2761 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     2821 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     2881 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     2941 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     3001 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     3061 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     3121 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     3181 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     3241 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     3301 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     3361 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     3421 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     3481 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga
     3541 ttcccacaca ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg
     3601 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     3661 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     3721 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     3781 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     3841 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     3901 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     3961 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     4021 ggttcgtgac agttagggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     4081 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     4141 gttttatttg atgcctttaa ttaaacgttc gtaatcaagc cacttccttt ttgctcactg
     4201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4261 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     4321 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     4381 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     4441 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     4501 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     4561 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     4621 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     4681 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     4741 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     4801 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     4861 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     4921 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     4981 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     5041 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     5101 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     5161 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     5221 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     5281 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     5341 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     5401 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     5461 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     5521 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     5581 cgccagcaac cgcataatgt gagttagctc actcattagg caccccaggc tttacacttt
     5641 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaattccc ctctaggatc
     5701 cgaattcgaa ggagatatac atatgacaaa aacatctaaa cttgacgcac ttagggctgc
     5761 tacttcacgt gaagacttgg ctaaaatttt agatattaag ttggtatttt taactaacgt
     5821 tctatataga atcggctcgg ataatcaata cactcaattt acaataccga agaaaggaaa
     5881 aggggtaagg actatttctg cacctacaga ccggttgaag gacatccaac gaagaatatg
     5941 tgacttactt tctgattgta gagatgagat ctttgctata aggaaaatta gtaacaacta
     6001 ttcctttggt tttgagaggg gaaaatcaat aatcctaaat gcttataagc atagaggcaa
     6061 acaaataata ttaaatatag atcttaagga tttttttgaa agctttaatt ttggacgagt
     6121 tagaggatat tttctttcca atcaggattt tttattaaat cctgtggtgg caacgacact
     6181 tgcaaaagct gcatgctata atggaaccct cccccaagga agtccatgtt ctcctattat
     6241 ctcaaatcta atttgcaata ttatggatat gagattagct aagctggcta aaaaatatgg
     6301 atgtacttat agcagatatg ctgatgatat aacaatttct acaaataaaa atacatttcc
     6361 gttagaaatg gctactgtgc aacctgaagg ggttgttttg ggaaaagttt tggtaaaaga
     6421 aatagaaaac tctggattcg aaataaatga ttcaaagact aggcttacgt ataagacatc
     6481 aaggcaagaa gtaacgggac ttacagttaa cagaatcgtt aatattgata gatgttatta
     6541 taaaaaaact cgggcgttgg cacatgcttt gtatcgtaca ggtgaatata aagtgccaga
     6601 tgaaaatggt gttttagttt caggaggtct ggataaactt gaggggatgt ttggttttat
     6661 tgatcaagtt gataagttta acaatataaa gaaaaaactg aacaagcaac ctgatagata
     6721 tgtattgact aatgcgactt tgcatggttt taaattaaag ttgaatgcgc gagaaaaagc
     6781 atatagtaaa tttatttact ataaattttt tcatggcaac acctgtccta cgataattac
     6841 agaagggaag actgatcgga tatatttgaa ggctgctttg cattctttgg agacatcata
     6901 tcctgagttg tttagagaaa aaacagatag taaaaagaaa gaaataaatc ttaatatatt
     6961 taaatctaat gaaaagacca aatatttttt agatctttct gggggaactg cagatctgaa
     7021 aaaatttgta gagcgttata aaaataatta tgcttcttat tatggttctg ttccaaaaca
     7081 gccagtgatt atggttcttg ataatgatac aggtccaagc gatttactta attttctgcg
     7141 caataaagtt aaaagctgcc cagacgatgt aactgaaatg agaaagatga aatatattca
     7201 tgttttctat aatttatata tagttctcac accattgagt ccttccggcg aacaaacttc
     7261 aatggaggat cttttcccta aagatatttt agatatcaag attgatggta agaaattcaa
     7321 caaaaataat gatggagact caaaaacgga atatgggaag catatttttt ccatgagggt
     7381 tgttagagat aaaaagcgga aaatagattt taaggcattt tgttgtattt ttgatgctat
     7441 aaaagatata aaggaacatt ataaattaat gttaaatagc taataccgtt catatgaggc
     7501 tagagcaggt gatacgcatg taggcagatt tgttggttgt gaatcgcaac cagtggcctt
     7561 aatggcagga ggaatcgcct ccctaaaatc cttgaagtga agagcgagtg tgagccgttg
     7621 tagcttcaac ctgtccctta cgaatagctc ttcaccccga aggagtgcct gcatgcgtgc
     7681 cacctgcatg atccgcggcc gcactcgatc gaaataattt tgtttaactt taaggaggtt
     7741 tggaatgaac caaatcgtga agttcactga cgactctggc ctggcggttc aagttactcc
     7801 agacgatgtt cgccgttata tctgtgagaa cgctactgaa aaagaggtgg gcctctttct
     7861 gcaactctgt cagactcaac gtctgaatcc gtttgtgaaa gacgcttacc tggtgaaata
     7921 cggcggtgct ccagcttcta tgattacttc ctatcaagtt tttaaccgtc gcgcgtgtcg
     7981 tgatgctaac tatgatggta tcaaatctgg tgtggttgtt ctgcgtgacg gtgatgttgt
     8041 gcataaacgt ggtgctgcgt gctacaaaaa ggcgggtgag gagctcatcg gtggttgggc
     8101 ggaagttcgc tttaaggatg gccgcgagac tgcgtatgct gaggtggcgc tcgacgacta
     8161 ttccaccggc aaatctaatt gggcgaaaat gccgggtgtt atgatcgaaa aatgcgcgaa
     8221 ggctgctgct tggcgcctcg cgttcccgga cacttttcag ggcatgtacg ctgcggagga
     8281 aatggatcaa gcgcaacagc cagaacaggt gcgcgctcag gcggagcaac cagtggatct
     8341 ccagccaatc cgcgaactct tcaagccata ttgcgaacac ttcggcatca ctccggctga
     8401 gggtatgact gctgtttgtg gtgcggtggg cgctgaaggc atgcactcta tgaccgagca
     8461 gcaagctcgc cgtgctcgcg cttggatgga ggaagaaatg gctgcgccag ctgtggaagc
     8521 ggagtatgag gttgttgacg agggcgaggt gttttaagga tccaggagaa tacacatggc
     8581 cagcagaggc gtaaacaagg ttattctcgt tggtaatctg ggtcaggacc cggaagtacg
     8641 ctacatgcca aatggtggcg cagttgccaa cattacgctg gctacttccg aatcctggcg
     8701 tgataaagcg accggcgaga tgaaagagca gactgaatgg caccgcgttg tgctgttcgg
     8761 caaactggca gaagtggcca gcgaatatct gcgtaaaggt tctcaggttt atatcgaagg
     8821 tcagctgcgt acccgtaaat ggaccgatca atccggtcag gatcgctaca ccacagaagt
     8881 cgtggtgaac gttggcggca ccatgcagat gctgggtggt cgtcagggtg gtggcgctcc
     8941 ggcaggtggt aatatcggtg gtggtcagcc gcagggcggt tggggtcagc ctcagcagcc
     9001 gcagggtggc aatcagttca gcggcggcgc gcagtctcgc ccgcagcagt ccgctccggc
     9061 agcgccgtct aacgagccgc cgatggactt tgatgatgac attccgttct gatctagagt
     9121 cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt
     9181 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     9241 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
     9301 gatttaaatt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     9361 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     9421 ttcagcgtga aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt
     9481 atggctataa atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt
     9541 atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg
     9601 atgtgaccga tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca
     9661 ttaaacattt tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca
     9721 aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc
     9781 tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg
     9841 atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga
     9901 gcgattttga tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata
     9961 aactgctgcc gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc
    10021 tgattttcga tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg
    10081 atcgttatca ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc
    10141 aaaaac
//
