LOCUS       sequence-482508-        9651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Hcl recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Hcl1
FEATURES             Location/Qualifiers
     source          1..9651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      613..707
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(828..847)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             839..1654
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1821..1929
                     /note="incP origin of transfer"
     CDS             complement(1940..2791)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2778..3617)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4128..5099)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARA*LARAPAPRQRGMDRGGPDFSM"
     rep_origin      5206..5600
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5638..5724)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5769..6851)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6813..6832
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6852..6929)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7135..7154
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    7169..7190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7205..7210,7211..7228,7229..7235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7239..7263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8810..8818
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cacatggcca gcagaggcgt aaacaaggtt attctcgttg gtaatctggg tcaggacccg
       61 gaagtacgct acatgccaaa tggtggcgca gttgccaaca ttacgctggc tacttccgaa
      121 tcctggcgtg ataaagcgac cggcgagatg aaagagcaga ctgaatggca ccgcgttgtg
      181 ctgttcggca aactggcaga agtggccagc gaatatctgc gtaaaggttc tcaggtttat
      241 atcgaaggtc agctgcgtac ccgtaaatgg accgatcaat ccggtcagga tcgctacacc
      301 acagaagtcg tggtgaacgt tggcggcacc atgcagatgc tgggtggtcg tcagggtggt
      361 ggcgctccgg caggtggtaa tatcggtggt ggtcagccgc agggcggttg gggtcagcct
      421 cagcagccgc agggtggcaa tcagttcagc ggcggcgcgc agtctcgccc gcagcagtcc
      481 gctccggcag cgccgtctaa cgagccgccg atggactttg atgatgacat tccgttctga
      541 tctagagtcg acctgcaggc atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg
      601 cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      661 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca
      721 ataattacga tttaaatttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      781 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      841 gagccatatt cagcgtgaaa cgagctgtag ccgtccgcgt ctgaacagca acatggatgc
      901 ggatctgtat ggctataaat gggcgcgtga taacgtgggt cagagcggcg cgaccattta
      961 tcgtctgtat ggcaaaccgg atgcgccgga actgtttctg aaacatggca aaggcagcgt
     1021 ggcgaacgat gtgaccgatg aaatggtgcg tctgaactgg ctgaccgaat ttatgccgct
     1081 gccgaccatt aaacatttta ttcgcacccc ggatgatgcg tggctgctga ccaccgcgat
     1141 tccgggcaaa accgcgtttc aggtgctgga agaatatccg gatagcggcg aaaacattgt
     1201 ggatgcgctg gccgtgtttc tgcgtcgtct gcatagcatt ccggtgtgca actgcccgtt
     1261 taacagcgat cgtgtgtttc gtctggccca ggcgcagagc cgtatgaaca acggcctggt
     1321 ggatgcgagc gattttgatg atgaacgtaa cggctggccg gtggaacagg tgtggaaaga
     1381 aatgcataaa ctgctgccgt ttagcccgga tagcgtggtg acccacggcg attttagcct
     1441 ggataacctg attttcgatg aaggcaaact gattggctgc attgatgtgg gccgtgtggg
     1501 cattgcggat cgttatcagg atctggccat tctgtggaac tgcctgggcg aatttagccc
     1561 gagcctgcaa aaacgtctgt ttcagaaata tggcattgat aatccggata tgaacaaact
     1621 gcaatttcat ctgatgctgg atgaattttt ctaataatta attggaccgc ggtccgcgcg
     1681 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     1741 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     1801 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     1861 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     1921 ggctggccgt aggccggcct cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg
     1981 ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc
     2041 gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca tagccgcaca
     2101 aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac agccgctggt
     2161 gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg atgcgcacat
     2221 gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc
     2281 cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc ttgcggccat
     2341 tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg
     2401 ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag ctacctttga
     2461 ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc cggagctgcc
     2521 gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca gccatggcca
     2581 ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg ctgaactcga
     2641 tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc
     2701 gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgc cggacgtggc
     2761 tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt gcgctgcctc
     2821 tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg atcagcgaac
     2881 ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg ctggtcgtcg
     2941 tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg
     3001 atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc
     3061 atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt atcggcggcg
     3121 atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc ttcctcgatg
     3181 tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc gcgcttgagg
     3241 ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg ctggatcagc
     3301 aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg
     3361 cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc
     3421 agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag ttgcagggcc
     3481 agcatggatt taccggcacc accgggcgac accagcgccc cgaccgtacc ggccaccatg
     3541 ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga acgcctccag aatattgata
     3601 ggcttatggg tagccattga ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg
     3661 ggtcactacc cccgccctgc gccgctctga gttcttccag gcactcgcgc agcgcctcgt
     3721 attcgtcgtc ggtcagccag aacttgcgct gacgcatccc tttggccttc atgcgctcgg
     3781 catatcgcgc ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct
     3841 tttggtcttt catatcagtc accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg
     3901 aacaaggaca aggtgcagcc gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg
     3961 ccagcctcgg ccttgtttga cgtataacca aagccaccgg gcaaccaata gcccttgtca
     4021 cttttgatca ggtagaccga ccctgaagcg cttttttcgt attccataaa acccccttct
     4081 gtgcgtgagt actcatagta taacaggcgt gagtaccaac gcaagcacta catgctgaaa
     4141 tctggcccgc ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc cagctaggcc
     4201 cgcgcaagct ggacgctggg cagacccatg accttgctga cggtgcgctc gatgtaatcc
     4261 gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg cctcggccat ggccttgccg
     4321 atttcctcgg cactgcggcc ccggctggcc agcttctgcg cggcgataaa gtcgcacttg
     4381 ctgaggtcat caccgaagcg cttgaccagc ccggccatct cgctgcggta ctcgtccagc
     4441 gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt cgaggctggc cagcctgcgg
     4501 gccttctcct gctgccgctg ggcctgctcg atctgctggc cagcctgctg caccagcgcc
     4561 gggccagcgg tggcggtctt gcccttggat tcacgcagca gcacccacgg ctgataaccg
     4621 gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg
     4681 ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc
     4741 cgaagttcac cgcctgcggc gtcggccacc ttgacccatg cctgatagtt cttcgggctg
     4801 gtttccacta ccagggcagg ctcccggccc tcggctttca tgtcatccag gtcaaactcg
     4861 ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct cggcgggcct gatatacacg
     4921 tcattgccct gggcattcat ccgcttgagc catggcgtgt tctggagcac ttcggcggct
     4981 gaccattccc ggttcatcat ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc
     5041 tcacagccca tggccttgag ctgtcggcct atggcctgca aagtcctgtc gttcttcatc
     5101 gggccaccaa gcgattccca cacattatac gagccggaag cataaagtgt agctagatcc
     5161 gaaggatgag ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc
     5221 cccacccttc gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc
     5281 tgcggggttt ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg
     5341 tggggcggtg tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat
     5401 attgggcaag ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc
     5461 ttagataatc atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg
     5521 caggtttggg ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg
     5581 acagttattg caggggttcg tgacagttag ggcgcgccca gctgtctagg gcggcggatt
     5641 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     5701 cgactgagcc tttcgtttta tttgatgcct ttaattaaac gttcgtaatc aagccacttc
     5761 ctttttgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5821 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     5881 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
     5941 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     6001 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     6061 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     6121 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     6181 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     6241 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     6301 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     6361 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     6421 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     6481 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     6541 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     6601 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     6661 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     6721 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     6781 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     6841 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     6901 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
     6961 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
     7021 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     7081 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     7141 gtcggcgata taggcgccag caaccgcata atgtgagtta gctcactcat taggcacccc
     7201 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7261 tcccctctag gatccgaatt cgaaggagat atacatttgt caatgattca aaaagattat
     7321 acagatagtt ttattttaaa tgctttgaat ctaccaacta tttcagattt agattcgtta
     7381 tctgatgctt ttgcaatcag taaaagactt atctttttat taaccaaaaa aacagaaaac
     7441 tattacaaaa gattttatat taaaaagaga gatggtacaa gtagagagat acttagtccc
     7501 acatattctt taaaattaat acaacgatgg attttgaaag aaatattgga gaaagttagt
     7561 ttatcagagc aagcgatggc atataaaaaa gggaaaaata atggcataaa gaaaaatgca
     7621 atgcatcata gatacagcct atatatactt gaaatggata ttaaagattt tttccattca
     7681 ataaaaagag aaagagtgtt ttatcttttt aagaacttgg gctataacaa tatggtttca
     7741 aatatcttaa ctaatctatg tacttataat gggtatttac ctcaaggggg agtttgctcg
     7801 ccttatattt ccaatttaat ttgttacagg ttagataatc ggttgtcagg attatgtgga
     7861 aaaagggata ttctttatac aagatatgct gacgatttaa ccttttcaag tgataataaa
     7921 cagacactta ctaaagcgat aaatattata attaacataa ttgaggatga aggatttaaa
     7981 gtcaataaaa ataagacaag actgctatct ccaggttcac acaaaaaagt tactggaatt
     8041 actgtgaatg atagtaaaat aaaggcaagt aagcaattaa aacgaactgt tagagcaatg
     8101 atacatcagg caatagttac agccgattat agccagattg atagaattag agggatagta
     8161 tcttatataa attccattga agttggatat agggaaaaag ttataactta cataaatagt
     8221 ttatcaaata aggattacgc attttttgag gaaatagttg agcggtttaa tgacaacaag
     8281 atttttaagg aaatacaaga tatgaattat aaaggatatg attacgatga ccaggttagt
     8341 ctggagttag aaaaaatagt gagtgccagg caagaatatc tcggaaaaat tggaaaagtg
     8401 gactttttgt cagcacatat acaaagtcaa caagatgagt tcaaaacaaa tgctgctgca
     8461 actaaagaaa cttatgaata gtaccgttca tatgaggcta gagcaggtga tattattgac
     8521 aacatcgtta tccttagatg ctactacttt tgtataaacg tgcacaatat ttatttatat
     8581 gaaagtagtg ggaagtggtt cgctttccga ggtgctatgt caggactata tttaatgaag
     8641 taagaagtga agagcgagtg tgagccgttg tagcttcaac ctgtccctta cgaatagctc
     8701 ttcacccctt acttcatcaa aaggaataaa ggtaaggagg acggtgttgt caatgatgcc
     8761 acctgcatga tccgcggccg cactcgatcg aaataatttt gtttaacttt aaggaggttt
     8821 ggaatgaacc aaatcgtgaa gttcactgac gactctggcc tggcggttca agttactcca
     8881 gacgatgttc gccgttatat ctgtgagaac gctactgaaa aagaggtggg cctctttctg
     8941 caactctgtc agactcaacg tctgaatccg tttgtgaaag acgcttacct ggtgaaatac
     9001 ggcggtgctc cagcttctat gattacttcc tatcaagttt ttaaccgtcg cgcgtgtcgt
     9061 gatgctaact atgatggtat caaatctggt gtggttgttc tgcgtgacgg tgatgttgtg
     9121 cataaacgtg gtgctgcgtg ctacaaaaag gcgggtgagg agctcatcgg tggttgggcg
     9181 gaagttcgct ttaaggatgg ccgcgagact gcgtatgctg aggtggcgct cgacgactat
     9241 tccaccggca aatctaattg ggcgaaaatg ccgggtgtta tgatcgaaaa atgcgcgaag
     9301 gctgctgctt ggcgcctcgc gttcccggac acttttcagg gcatgtacgc tgcggaggaa
     9361 atggatcaag cgcaacagcc agaacaggtg cgcgctcagg cggagcaacc agtggatctc
     9421 cagccaatcc gcgaactctt caagccatat tgcgaacact tcggcatcac tccggctgag
     9481 ggtatgactg ctgtttgtgg tgcggtgggc gctgaaggca tgcactctat gaccgagcag
     9541 caagctcgcc gtgctcgcgc ttggatggag gaagaaatgg ctgcgccagc tgtggaagcg
     9601 gagtatgagg ttgttgacga gggcgaggtg ttttaaggat ccaggagaat a
//
