LOCUS       sequence-482510-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Tsp1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Tsp1
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             701..709
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2155..2249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2370..2389)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2381..3196
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3363..3471
                     /note="incP origin of transfer"
     CDS             complement(3482..4333)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4320..5159)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5670..6641)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6748..7142
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(7180..7266)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7311..8393)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8355..8374
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8394..8471)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8677..8696
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    8711..8732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8747..8752,8753..8770,8771..8777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8781..8805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgctacgccg acgatttaac cttttccgcc tctggcgaca gtctgcgcca tatatgtaat
       61 atactcaaag gcacagaatc aattgttacc cacgaaggct ttaccatcaa ccaagaaaaa
      121 acccgaattt tgcgaaaatc tcgccagcaa gaagttacgg gagtggtggt aaacgataaa
      181 cccaacgttg acaacaaaac attgaaacgc tttcgcgcta ccctgtttca aattgagaaa
      241 gatggtttag aaggaaagca ctggggcaac tctgataatt taatcgctgc tattggtggc
      301 tttgccaatt ttgtcgcaat ggtaaatccc gaaaaaggtg cggagtttca acagcaagtg
      361 caacgcatca aaaataagta tgttcgcaac ggtagaagtt agtaccgttc atatgaggct
      421 agagcaggtg attcacgcgc atctgtaagc tacgcgaaac aaccttgcaa accggacgat
      481 ggttcgtcgt ccttgtgctt agtctaacca tgcatcacta gcctgctcaa gtgaagagcg
      541 agtgtgagcc gttgtagctt caacctgtcc cttacgaata gctcttcacc cgagcagtct
      601 agtcctgaga cctttacgaa ggggtaattt gcacttcgat gcgcgtgagc cacctgcatg
      661 atccgcggcc gcactcgatc gaaataattt tgtttaactt taaggaggtt tggaatgaac
      721 caaatcgtga agttcactga cgactctggc ctggcggttc aagttactcc agacgatgtt
      781 cgccgttata tctgtgagaa cgctactgaa aaagaggtgg gcctctttct gcaactctgt
      841 cagactcaac gtctgaatcc gtttgtgaaa gacgcttacc tggtgaaata cggcggtgct
      901 ccagcttcta tgattacttc ctatcaagtt tttaaccgtc gcgcgtgtcg tgatgctaac
      961 tatgatggta tcaaatctgg tgtggttgtt ctgcgtgacg gtgatgttgt gcataaacgt
     1021 ggtgctgcgt gctacaaaaa ggcgggtgag gagctcatcg gtggttgggc ggaagttcgc
     1081 tttaaggatg gccgcgagac tgcgtatgct gaggtggcgc tcgacgacta ttccaccggc
     1141 aaatctaatt gggcgaaaat gccgggtgtt atgatcgaaa aatgcgcgaa ggctgctgct
     1201 tggcgcctcg cgttcccgga cacttttcag ggcatgtacg ctgcggagga aatggatcaa
     1261 gcgcaacagc cagaacaggt gcgcgctcag gcggagcaac cagtggatct ccagccaatc
     1321 cgcgaactct tcaagccata ttgcgaacac ttcggcatca ctccggctga gggtatgact
     1381 gctgtttgtg gtgcggtggg cgctgaaggc atgcactcta tgaccgagca gcaagctcgc
     1441 cgtgctcgcg cttggatgga ggaagaaatg gctgcgccag ctgtggaagc ggagtatgag
     1501 gttgttgacg agggcgaggt gttttaagga tccaggagaa tacacatggc cagcagaggc
     1561 gtaaacaagg ttattctcgt tggtaatctg ggtcaggacc cggaagtacg ctacatgcca
     1621 aatggtggcg cagttgccaa cattacgctg gctacttccg aatcctggcg tgataaagcg
     1681 accggcgaga tgaaagagca gactgaatgg caccgcgttg tgctgttcgg caaactggca
     1741 gaagtggcca gcgaatatct gcgtaaaggt tctcaggttt atatcgaagg tcagctgcgt
     1801 acccgtaaat ggaccgatca atccggtcag gatcgctaca ccacagaagt cgtggtgaac
     1861 gttggcggca ccatgcagat gctgggtggt cgtcagggtg gtggcgctcc ggcaggtggt
     1921 aatatcggtg gtggtcagcc gcagggcggt tggggtcagc ctcagcagcc gcagggtggc
     1981 aatcagttca gcggcggcgc gcagtctcgc ccgcagcagt ccgctccggc agcgccgtct
     2041 aacgagccgc cgatggactt tgatgatgac attccgttct gatctagagt cgacctgcag
     2101 gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc
     2161 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     2221 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     2281 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2341 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcagcgtga
     2401 aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt atggctataa
     2461 atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc
     2521 ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga
     2581 tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca ttaaacattt
     2641 tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca aaaccgcgtt
     2701 tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt
     2761 tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt
     2821 tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga gcgattttga
     2881 tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc
     2941 gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc tgattttcga
     3001 tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg atcgttatca
     3061 ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc aaaaacgtct
     3121 gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc atctgatgct
     3181 ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt ttccgctgca
     3241 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     3301 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     3361 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     3421 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc
     3481 ctcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg
     3541 tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca
     3601 tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct atggaagcct
     3661 tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg cgggcggttt
     3721 cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg ccacccatga
     3781 cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg
     3841 cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata
     3901 ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct
     3961 ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg
     4021 tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg
     4081 gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc
     4141 gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt
     4201 agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca
     4261 ggccgggggc cagacagtgc gccgggtcgt gccggacgtg gctgaggctg tgcttgttct
     4321 taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg
     4381 agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc atagttggcc
     4441 ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg
     4501 gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc gaccagtacc
     4561 gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc gcccttgctg
     4621 gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc catgcgaccg
     4681 atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg
     4741 tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg
     4801 gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc agccacggct
     4861 tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg
     4921 ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc cacctccagc
     4981 agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga tttaccggca
     5041 ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa aacgtagtcc
     5101 agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg ggtagccatt
     5161 gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta cccccgccct
     5221 gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc
     5281 agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc gcttggcgta
     5341 cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct ttcatatcag
     5401 tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag
     5461 ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc ggccttgttt
     5521 gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat caggtagacc
     5581 gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga gtactcatag
     5641 tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc gcccctgtcc
     5701 atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg
     5761 ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg
     5821 cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc ggcactgcgg
     5881 ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc atcaccgaag
     5941 cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg
     6001 ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc gggccttctc ctgctgccgc
     6061 tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc ggtggcggtc
     6121 ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt ggtgtgcttg
     6181 tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc gctggccggg
     6241 tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc accgcctgcg
     6301 gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac taccagggca
     6361 ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc gtccaccagc
     6421 accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc ctgggcattc
     6481 atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc ccggttcatc
     6541 atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc catggccttg
     6601 agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc aagcgattcc
     6661 cacacattat acgagccgga agcataaagt gtagctagat ccgaaggatg agccgggctg
     6721 aatgatcgac cgagacaggc cctgcggggc tgcacacgcg cccccaccct tcgggtaggg
     6781 ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg
     6841 gtttagcggg ctttgcccgc ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct
     6901 agcgcagcga atagaccagc tatccggcct ctggccgggc atattgggca agggcagcag
     6961 cgccccacaa gggcgctgat aaccgcgcct agtggattat tcttagataa tcatggatgg
     7021 atttttccaa caccccgcca gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac
     7081 agttattgca ggggttcgtg acagttattg caggggggcg tgacagttat tgcaggggtt
     7141 cgtgacagtt agggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     7201 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     7261 tatttgatgc ctttaattaa acgttcgtaa tcaagccact tcctttttgc tcactgcccg
     7321 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7381 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     7441 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     7501 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     7561 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     7621 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     7681 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     7741 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     7801 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     7861 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     7921 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     7981 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     8041 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     8101 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     8161 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     8221 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     8281 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     8341 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     8401 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     8461 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     8521 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     8581 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     8641 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     8701 agcaaccgca taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8761 ttccggctcg tatgttgtgt ggaattgtga gcggataaca attcccctct aggatccgaa
     8821 ttcgaaggag atatacatat gtctgaccaa ccacgtaccc gccaagaact atacgatcgc
     8881 attcgacaag ttggcaaaga ggaatttatc cttgaagaaa tgatacgtta tggtttctgg
     8941 tctgcacaag gcgaaatgcc gcaagatcca gcagatgaaa ttcgccgcgc tggagaaatt
     9001 cgtcgggaac tgaacgaact gcggcaagaa agtcgaaaat tacaagatga aaaggaatta
     9061 cggaagcgct tattaaagca gcgtttagaa gaatccaaac gcaagcgtca agaaacaaaa
     9121 gaacgccgcg aacaagaacg gcgatcgcgt gcagaagctt ggaagcagaa acagcagcag
     9181 gatattatct atcttgggga ggatgtatct gctgggttaa ataacactca aggcaatgaa
     9241 gaaaggttgc attcctacaa tttaccgcta tgcaacaacg ccgaacaaat tgccgctgcg
     9301 atgggaatca ctataggtaa actccgcttt ttggcattta accgcaaaac ctcgacagtt
     9361 tcgcactaca tccgcttcaa aatgccgaaa aagacggggg gagaaagaat aatttccgca
     9421 ccgatgccga atttaaaaca ggcacagcac tggattttag cgaatatttt ggaaaagctg
     9481 gaacttcacg acgctgcaca tggctttaga cgcgatcgct ctatcgtcac caatgctaca
     9541 ccccatgtcg gcgctgatgt aattatcaac ttcgatttaa agaacttttt cccctccatt
     9601 tcttataaac gagtcaaagg tttatttcac tccttcggct attccgaagc agcggcgaca
     9661 attttcggct tggtgtctac cgctgctgaa gtcgaaactt tagaaattga cggcaaaact
     9721 tattatgttg cagtcgatga acgtcacctt ccccaaggtt cccccgcaag tccagcaatt
     9781 actaacttac tctgtcgccg cttagaccga cgtttaacgg cgatggcaga aaaattaggt
     9841 tttacttaca cc
//
