LOCUS       sequence-482513-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Kva1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Kva1
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(294..388)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1016..1801)
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     protein_bind    3186..3210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3214..3220,3221..3238,3239..3244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3259..3280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3295..3314)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3520..3597
                     /gene="lacI"
                     /label=lacI promoter
     CDS             3598..4680
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3617..3636)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      4725..4811
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4849..5243
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5350..6321
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6832..7671
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7658..8509
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     oriT            8520..8628
                     /note="incP origin of transfer"
     CDS             complement(join(8795..9448,1..162))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cagaaacagt tccggcgcat ccggtttgcc atacagacga taaatggtcg cgccgctctg
       61 acccacgtta tcacgcgccc atttatagcc atacagatcc gcatccatgt tgctgttcag
      121 acgcggacgg ctacagctcg tttcacgctg aatatggctc ataacacccc ttgtattact
      181 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
      241 acatcagaga ttttgagaca caaatttaaa tcgtaattat tggggacccc tggattctca
      301 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      361 gtcattactg gatctatcaa caggagtcca agactagtcg ccagggtttt cccagtcacg
      421 acgcggccgc aagcttgcat gcctgcaggt cgactctaga tcagaacgga atgtcatcat
      481 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
      541 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct
      601 gcggctgacc accaccgata ttaccacctg ccggagcgcc accaccctga cgaccaccca
      661 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
      721 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
      781 gcagatattc gctggccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
      841 tctgctcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
      901 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
      961 caacgagaat aaccttgttt acgcctctgc tggccatgtg tattctcctg gatcctcatg
     1021 ctgccacctt ctgctctgcg gctttctgtt tcaggaatcc aagagctttt actgcttcgg
     1081 cctgtgtcag ttctgacgat gcacgaatgt cgcggcgaaa tatctgggaa cagagcggca
     1141 ataagtcgtc atcccatgtt ttatccaggg cgatcagcag agtgttaatc tcctgcatgg
     1201 tttcatcgtt aaccggagtg atgtcgcgtt ccggctgacg ttctgcagtg tatgcagtat
     1261 tttcgacaat gcgctcggct tcatccttgt catagatacc agcaaatccg aaggccagac
     1321 gggcacactg aatcatggct ttatgacgta acatccgttt gggatgcgac tgccacggcc
     1381 ccgtgatttc tctgccttcg cgagttttga atggttcgcg gcggcattca tccatccatt
     1441 cggtaacgca gatcggatga ttacggtcct tgcggtaaat ccggcatgta caggattcat
     1501 tgtcctgctc aaagtccatg ccatcaaact gctggttttc attgatgatg cgggaccagc
     1561 catcaacgcc caccaccgga acgatgccat tctgcttatc aggaaaggcg taaatttctt
     1621 tcgtccacgg attaaggccg tactggttgg caacgatcag taatgcgatg aactgcgcat
     1681 cgctggcatc acctttaaat gccgtctggc gaagagtggt gatcagttcc tgtgggtcga
     1741 cagaatccat gccgacacgt tcagccagct tcccagccag cgttgcgagt gcagtactca
     1801 ttagaaaacc tccatagcat gattaagatg tttcagtacg aaaattgctt tcattgttga
     1861 tctccttttt agcggccgcg gatcatgcag gtggcagccg cttatgttat aaaaaacccc
     1921 ggtaaaccgg ggttctgctc tttacctaga agggcacggc ctgggtgaag agctattcgt
     1981 aagggacagg ttgaagctac aacggctcac actcgctctt cacttaggcc gtggcataaa
     2041 tgaccacaat caacaagttc cggtgataca ccagatgaag cagatacgcc tctatcgcgt
     2101 aaagagtatg ttatacacta acttaatgat ttgcatcaat caagctgctt gtctcaagat
     2161 tatggatcac ctgctctagc ctcatatgaa cggtattagt cattgttttt ctgcaggact
     2221 gcgaagctat ccttacccag ttgtttcagt ttgtcctcac cgttaatttt aatcaggcta
     2281 tagtaatagt ccggttcgat atttttgatg tgagacagca tgcccaggag gtacggaatg
     2341 tccttatgat tgattttgtt attgctgtac aggtaaaaca gtgcgcggat tttcttacgc
     2401 agaaaacggc ccacacccag atcgccggtg ttagaaataa ccacaccagt aatgcgacgg
     2461 ctacgacctt taccaataaa tttggtttta gaagtgttga taatcagttc tggtttcttc
     2521 tgcagagaca ccaggttaat aacgaagttt ttgacatcgt tcagtttgtc aaaagaatcg
     2581 gaagaaaaag acaggtcatc agcgtaacga gtgtagatca catcgttttc tttacagtag
     2641 ttgaacagtt tttcgtcgaa gtcgtacata acgatgttgg agatcatcgg agagctcggc
     2701 gcaccgatgc acaggcgcag tttcttttca cctttcttgc gacgaaacag gtagtggcta
     2761 attacttcat attcaaattg gttcagttta atatcgtttt tgctgataaa atagttcaga
     2821 tcctgcggtt tgatgctgta gaaaaaattt tcgaaatcca ttttgaggat gtattcgttt
     2881 ttcttgtgcg gttctgcgtt atcaacaatg gatttttcct cgatatacgc ggtcgcgcac
     2941 tcgtgaaccg gcaggagaga caggaggctg ttaacaatag cacgttgaac tttcttaacg
     3001 tccggagacg gctgcgcgat ctcacggagg cctttaccgt ttttcttttt gataaagaac
     3061 accttgtatt tcttaggcgc cttgatgtag ccagagataa tagtcttcgg agtgcagttc
     3121 agagtcttgc tcagctcatc gatataggac atatgtatat ctccttcgaa ttcggatcct
     3181 agaggggaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3241 taaagcctgg ggtgcctaat gagtgagcta actcacatta tgcggttgct ggcgcctata
     3301 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     3361 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     3421 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     3481 taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa
     3541 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     3601 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     3661 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     3721 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     3781 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     3841 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     3901 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     3961 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     4021 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     4081 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     4141 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     4201 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     4261 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     4321 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     4381 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     4441 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     4501 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     4561 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     4621 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4681 gcaaaaagga agtggcttga ttacgaacgt ttaattaaag gcatcaaata aaacgaaagg
     4741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     4801 gtaggacaaa tccgccgccc tagacagctg ggcgcgccct aactgtcacg aacccctgca
     4861 ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc
     4921 caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga
     4981 ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct
     5041 tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca
     5101 ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa
     5161 accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga
     5221 agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct
     5281 catccttcgg atctagctac actttatgct tccggctcgt ataatgtgtg ggaatcgctt
     5341 ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct caaggccatg
     5401 ggctgtgagc gcttcgatat cggcgtcagg gacgccacca ccggccagat gatgaaccgg
     5461 gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat gaatgcccag
     5521 ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt gctggtggac
     5581 gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc tgccctggta
     5641 gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt
     5701 gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga cccggccagc
     5761 gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga caagcacacc
     5821 acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc
     5881 gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca gcggcagcag
     5941 gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg
     6001 cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg cttcggtgat
     6061 gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc
     6121 gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg caagcccggc
     6181 cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag
     6241 cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat ggacaggggc
     6301 gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata ctatgagtac
     6361 tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc ggtctacctg
     6421 atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc
     6481 gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg
     6541 tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa
     6601 agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc
     6661 gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga
     6721 cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg
     6781 tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc
     6841 cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt
     6901 ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa
     6961 tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg
     7021 ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt
     7081 catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct
     7141 gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg
     7201 ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc
     7261 cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg
     7321 gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc
     7381 gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc
     7441 gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg
     7501 ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat
     7561 ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc
     7621 gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca
     7681 cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc
     7741 cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg
     7801 caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca
     7861 agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga
     7921 aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc
     7981 catggtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag
     8041 tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat
     8101 cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga
     8161 ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg
     8221 cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg
     8281 cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat
     8341 agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg
     8401 ccgccagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga
     8461 gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgag gccggcctac
     8521 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     8581 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     8641 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     8701 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     8761 aaggacaacg cgcggaccgc ggtccaatta attattagaa aaattcatcc agcatcagat
     8821 gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac agacgttttt
     8881 gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat
     8941 ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca
     9001 ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt
     9061 tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc
     9121 tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat
     9181 cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca
     9241 gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt
     9301 tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa
     9361 tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat
     9421 cgttcgccac gctgcctttg ccatgttt
//
