LOCUS       sequence-48266-e       15293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15293)
  TITLE     Kuwabara unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPK605
FEATURES             Location/Qualifiers
     source          1..15293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(13102..13120)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13141..13157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13141..13157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13154..13176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13165..13181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13189..13195,13196..13213,13214..13219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(13372..13389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13543..14131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14302..15093,15094..15162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14925..14944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15163..15267)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga ttctcggaaa
     7681 aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt tttcgtatnt
     7741 tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn aatttaattg
     7801 aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc ggttaataac
     7861 gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg acgtttttaa
     7921 atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat gacaaaggaa
     7981 taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac gtggattttt
     8041 ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag gattattgta
     8101 gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat tctcagaatt
     8161 ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca aaaacaattt
     8221 ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct aattatattt
     8281 gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc attaattaat
     8341 tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt ttatcgggaa
     8401 atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat tagtatttct
     8461 cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt cttgtatatt
     8521 tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa taatgaaaat
     8581 taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt tcaattggcg
     8641 gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa aatcatataa
     8701 catctagaca atttttttga atttttttat catgatattc ggtcattgtg tccccatagg
     8761 catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg tcaaaagaca
     8821 tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt ttggaggtga
     8881 aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag aaaatcgaca
     8941 tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact ggaaaatctg
     9001 ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat ttgttcgaaa
     9061 tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt ttttgcttgg
     9121 gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct tcagcgtacg
     9181 gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga aaatatcgga
     9241 aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga gaaaaatgct
     9301 tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa atctgtgagt
     9361 atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac caaattcgga
     9421 ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac gtttcttttc
     9481 gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat tatataaaaa
     9541 tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca attaaatata
     9601 attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta aataatcagt
     9661 gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct gtgagtcccg
     9721 caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggcggca
     9781 aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt ctttttgcag
     9841 tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagacgcgt ggatcctaat
     9901 tttgccgtat tttccatatt ttgttttgta tatttatcca ctcaccccct ctctttgtcc
     9961 tgtgaatgaa cttgtgccaa aaaagccaaa aataatttac tttttttaaa gacatattaa
    10021 aacttgaaaa cttggaacaa tacggtgcga aagcgaaaca gagacagaaa aatgaacaaa
    10081 gagatccgtg aaaagtgaac gatgatttag gcttcttgtt gggcggcgtg gatgagagtg
    10141 atgttgtctc ctttgagaag aattctgccg atcttgttgc gtcccttcgt cttcatgttc
    10201 acctcctcgg cttcgtcgaa cacgacattc atgaactcgt cgaatccgat gatgtagccc
    10261 tcgagacggt gggtgacatc ctcgtacaac cagatttgga cccgagtgcg gttttggaga
    10321 tatctgaaat ttgggaaatt gttttctact gattagtgaa aaaatattac ctgaagatga
    10381 ggttcacagg ctgaaccatc actttgttga gctttctcgt cgacattttt gcgggatgat
    10441 ctgtaaaaat caatatattt tttgaaagac acaaattttt aagataaata tcaagaaaac
    10501 tcgattttag cagctattta agctcaaaaa ctacaattta tggctttaaa acataatttt
    10561 tcacatttta cataaatttc aacgaaaaaa aatgccaaaa aacttcaaat ttgagcagaa
    10621 atcggctatt ttctcgacaa aaataatttc gaaaattcgt tcaaaaaaga tgagtacgca
    10681 agcaccgtga cgcacagttg cggactttgt aaaccgtacc cctgcataaa aaggcggaat
    10741 tttgaattta ataaaatgca gtttagaaca aagaaaagca cggagagaat tgtgataact
    10801 aattatttgt atttaatttt aaatactaca taaaactgaa gattggaacg agttaaagct
    10861 ggtatttgcg gtttatagcc ccaaaagtta aggcttgtta ggcaattatc gataaaaaca
    10921 acgagaaatt cgtgaaatcg gcaaaaattt atagaaaacg tttgaattaa attaaattta
    10981 cgcttttgcc gccaacacct atcgagaccc tagttatagg ggcggagcgt catgggagcg
    11041 cgcttgcaac tcgatttcaa aaagtttcgc tgtttgtaca acattctcag ttgttcttaa
    11101 tcagttaaat aagtttcaaa attttnncaa aatcataaaa cacagttaat gctaaattaa
    11161 agtctaaaaa natcaggtga taaggggttt ggacgaccaa aaagcgacct tcgctcgaaa
    11221 attgataaac taactacact ttttccggaa aaatgatgga aaaatcctga ttttcttcgt
    11281 tgaatcaatt tttgtttcat ttttaacact ttcacatgan naatatttca gttaaaatgc
    11341 tcaagctaat tgggtttctt ataattttgg gtctcgcaac ggccgacaag tgctcagatt
    11401 gtttggctca ggggaaacag tgagttggaa gcttacaatt ctgagaattc gtattgcgca
    11461 gcttatttga cgcgcaaaat atgaaatatc acgtagcgaa aactacagta attctttaaa
    11521 taagactact gtagtgccta tgtcgattta ctgcctcaac tttcttaatc aagctcgtaa
    11581 atcgacacca gctctacagt agtcatagtt ttcgctacga gatattttgc gcgtcaaata
    11641 tggtgtgcaa tacgcatttt cagaatttta tgttctcgta atataccttt tttcagctgg
    11701 tgtgttactg taaatgcatg tggatttacg ccatgtgtca ctacaattac agtgagtttt
    11761 tttgaagaaa aaaaaattac ccatttgatc gccaattcca tttttcaaaa atcaataaaa
    11821 aaaaaaaatt tcggcgatca gaaacgttac agaaagccct aaactgcccg gaactaccag
    11881 ataaagccca cgcttacgat gattcattta tgaggaataa ggtattgatc acacattctg
    11941 ctgcctacaa tatggaccca caaaagtgtt ttacgtaggt tcaccttcta gataaacctt
    12001 aaaaatcgat actttcagta gtaaaatgcc ttcgatgaaa gtttccaaaa cagttaatgt
    12061 caattgtgat agatatgggc caagtgagtt gttttattag cccacggatt tctggcttcc
    12121 ctcataaatt gaaaaggaag agattgccga actagaccat tttggctcgg cgcgttgcgt
    12181 gtcacgtcgc ggctcgattg taaaactaaa tgtattggtc cgtgtggagt acacgacttt
    12241 ccctttccgg caggcgattg tcaatggagc gcgaaaaatt caatgaggaa ggccagaacc
    12301 ccgtgtctgt agttttctca aaaagtggca aaaactcaat aattgccaat ttttgactgc
    12361 caataaaaat ttcactaaat ttattaaatt tattttaagt tttattgaaa tttttttaaa
    12421 atttccccac tggcgctaaa aaatccaatt ttcagaagca atgtgcttca gcttcacggc
    12481 catagatact gtccaaaaag tgattgcaat gacgtttaga gggacagaag gagcagctca
    12541 attgtctgaa gaaattttgg atttcttcca gtaagttcgg gaaatggcaa tttaaagttt
    12601 aattggattt ttgtattttt tcttctcacg actacagttt tgcacatttt tatgttataa
    12661 aaaaccaaaa aaaaattttt ttgccttttt tctcagctta ccacaaaaca atttcgacca
    12721 aaaataaatt gaaaaaatta aaaaattttt tgcacggttt tcggactcaa aaaaagctga
    12781 atgttcctgg aactatttgt aaaattcaaa attcaaaatt ttcacgaatt ctctaaaagg
    12841 ttccagcaaa ttccacaact ttcttgaatt tttcaaaaaa gttcttgagc attcaacaat
    12901 tatttttgga attttccaaa ggttacagga cgttccaatt tttcaaaaaa atttctcgca
    12961 aaaaatgttt tctcagaaaa tttgaatttc cctccacata aaagattgtt ctcaaaaaat
    13021 ttgcaaacac acattttcag cggaaaaaag gcgttcttcg actctggaag tgtattcgaa
    13081 tatggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg
    13141 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
    13201 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
    13261 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    13321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    13381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    13441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    13501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc
    13561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    13621 ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    13681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
    13741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    13801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    13861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    13921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    13981 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    14041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    14101 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    14161 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    14221 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    14281 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    14341 ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg
    14401 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    14461 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    14521 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    14581 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    14641 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    14701 ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    14761 gttggccgca gtgttatcac tcatgcttat ggcagcactg cataattctc ttactgtcat
    14821 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    14881 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    14941 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    15001 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    15061 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    15121 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    15181 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    15241 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
