LOCUS       sequence-48273-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, 
            Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT
  TITLE     Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin 
            signaling.
  JOURNAL   Science. 2007 May 18;316(5827):1043-6.
  PUBMED    17510365
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-05-18"; volume: "316(5827)"; pages: "1043-6"
COMMENT     Sequence Label: NC1 pGLUE WTX (1-804)
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..219
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             238..258
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             292..369
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gctactagag aaccccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tggtaccgag ctcggatcga tttaattaag ccaccatgga cgagaagacc
      121 accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga gcagctgcgg
      181 gcccggctgg agcaccaccc ccagggccag cgggagcccg gcggaagccc cggtggcgag
      241 aacctgtact tccagggcgc aagctacccc tacgacgtgc ccgactacgc caagcggcgg
      301 tggaagaaga acttcatcgc cgtgagcgcc gccaaccggt tcaagaagat cagcagcagc
      361 ggcgccctga ttgatggggg atctggcccc ggcggcggcg cgcctatgga gacccaaaag
      421 gatgaagctg ctcaggccaa gggagctgca gcctctggga gtacccgtga acaaacagca
      481 gaaaaaggag ccaagaacaa ggcagctgag gcgacagaag gaccaacctc agagccatcc
      541 tcatccggcc caggtaggct gaagaaaact gccatgaaac tctttggtgg caagaagggt
      601 atctgtactc tgcctagttt ctttggaggg ggacggagca aaggttctgg gaaaggcagc
      661 tccaagaaag gtctcagcaa gagcaagacc cacgatggcc tgagtgaagc agcccatggc
      721 cctgaagatg ttgtcagtga aggaactggc ttctccctgc ctttgcctga gttaccctgc
//
