LOCUS       sequence-48274-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, 
            Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT
  TITLE     Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin 
            signaling.
  JOURNAL   Science. 2007 May 18;316(5827):1043-6.
  PUBMED    17510365
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-05-18"; volume: "316(5827)"; pages: "1043-6"
COMMENT     Sequence Label: NC3 pCDNA3.1 FLAG WTX (1-804)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat ccgccaccat
      121 ggactacaag gacgacgacg acaagggcgg cgcgcctatg gagacccaaa aggatgaagc
      181 tgctcaggcc aagggagctg cagcctctgg gagtacccgt gaacaaacag cagaaaaagg
      241 agccaagaac aaggcagctg aggcgacaga aggaccaacc tcagagccat cctcatccgg
      301 cccaggtagg ctgaagaaaa ctgccatgaa actctttggt ggcaagaagg gtatctgtac
      361 tctgcctagt ttctttggag ggggacggag caaaggttct gggaaaggca gctccaagaa
      421 aggtctcagc aagagcaaga cccacgatgg cctgagtgaa gcagcccatg gccctgaaga
      481 tgttgtcagt gaaggaactg gcttctccct gcctttgcct gagttaccct gccaatttcc
      541 cagctctcag agtgcccatg gggctttgga gacaggctcc agatgtaaga catctgtggc
      601 tggagccaca gagaaagctg tggctgagaa gtttccctct atgcccaagc caaagaaagg
      661 cctaaaaggc ttttttagca gtatccgccg tcaccggaag agcaaggtca ctggggctga
      721 gcaaagtgag ccaggggcca aggggcctga gagggtcaga gccaggcctc atgagcacgt
      781 gagctcagcc cctcaggtgc cctgctttga ggagaccttc caagccccta
//
