LOCUS       sequence-482804-       11760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, 
            AraC, C1, and pBAD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Al'Abri IS, Haller DJ, Li Z, Crook N
  TITLE     Inducible directed evolution of complex phenotypes in bacteria.
  JOURNAL   Nucleic Acids Res. 2022 Jun 10;50(10):e58. doi: 10.1093/nar/gkac094.
  PUBMED    35150576
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2022-06-10"; volume: "50(10):e58. doi"; pages: "
            10.1093/nar/gkac094"
COMMENT     Sequence Label: ISA179: Tagatose pathway
FEATURES             Location/Qualifiers
     source          1..11760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             941..1600
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1007..1026)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1671..1705
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(6966..7844)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        7871..8155
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     8078..8097
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      8407..8478
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8494..8521
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    complement(11656..11680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagcc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacactct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgcg gatctttgat
      841 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      901 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      961 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1021 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1081 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1141 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1201 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1261 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1321 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1381 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1441 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1501 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1561 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     1621 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1681 tcggttgccg ccgggcgttt tttattggta ggataagaca gtgctcgttt ggacggtttc
     1741 agcggttgtt tatgtaggaa ttgtcatgct tgtatacagc atattggccg gtcgggaacc
     1801 gatacccgat gaaaagcccc atgacggtca tgctttagaa gagcatgtca aatgaattgc
     1861 tgagaaagcc ggatgttaaa gaacatccgg ctttttgtat tagtatctgt cattgctcat
     1921 gcacatcaaa atcttttctt ggacgatttt tttcatggct tctttggcgg gggccatata
     1981 ttttcttgga tcgcttgcat cggcgtgttc atgcaaatat cggcgcagtt tatctgaaaa
     2041 aggaattttt aattcagtcg cgatatttac ttttgcacag cctagcttga tgcactttct
     2101 aacatcttgc tctgaaattc ctgaggcgcc gtgcaggacg agcgggactg aaacccgttt
     2161 ttgaatcgcg gccagcctct caaaatcgat attgggctct tttgagtaaa gaccgtgggc
     2221 tgtaccgatt gccaccgcca gcgaatcgat tccggttttc tcgacgaact cttttgctgc
     2281 atccggatcg gtataataag cgtcggcttc atccacgatc aaatcgtctt cctgtccgcc
     2341 gagccttcca agctcggctt ctacagtggc atctttttgc cgggcatatt gaacgacact
     2401 cgccgtaatt gcggtattct cggcaaatgg atggtgcgaa gcatcaatca tgacagattt
     2461 cgtccccaat gataaagact cgcgaatatc atcaatttct tcatgatgat ctagatggag
     2521 ggcgaccggg atcgagcagc gcgccgcggc cgtttctgca atggcttgaa tatagtcgcg
     2581 ccctgcatag ctgaaggttc ctggagtagc cgcaagaatc acgggggacc ttaaggcttc
     2641 agccgcttct gtcacggcct gcacggtttc aaggttatga atgttaaagg ccggcaccgc
     2701 atagccgttt gcctgtgcgt ccagcagcat ttttttcgta tttgtcagca tatcgattcc
     2761 ttctttctga tcaaagttaa gataaatagc ttgagaaggt gctcagaatg tcgtcaggac
     2821 tcgaggcttc aagcagctgc tgcctcgcat cttcgtgaac aagcatgcga gacaccgatg
     2881 ataatagatt gagatgtgtg ctgcctgcct cggcatcggg tatcaataat gcaatgatga
     2941 acgtgacagg cgtctgatca agcgcgttcc attcgattcc tgccttgttt tgaatgatga
     3001 tgacagttgc ttctgtgatg tccttcagtt ttgcatgggg aatggcgaag ccgtcgataa
     3061 agccggtcgt gctttcagcc tctctttgcg ccaatccgtc aatgaccgct tgttttgttt
     3121 tgactgctcc tgattcatat gcgatctgag ccagccggtc aaaagcttcc gcttgggaag
     3181 atagcgtttc ttgtagatag atgtgctctt ttttgattaa agctgctgtt ttcatgtttg
     3241 atcagccttt ctctttttca tatgtccgtt tttggtaaaa gccgtataag aaggcaccga
     3301 taagcgatcc gatgataatc gacagcaccc attgaaaggc tcctgttaca acgggaagta
     3361 caaggaatcc gccgtgcggg gcaggtactt gaactgcgaa tgaatatgtt aatattgccg
     3421 aaaccgaaga gccgatcatc aaaatcggaa tgacagggat tggtttcttt gcggcgaacg
     3481 ggatagcgcc ttcagtaata tgagtggcgc cgagaataaa attgacaagt cctgcatttc
     3541 ggtcctgctg gctgaaatat ttgcggaaaa gcaatgtggc aaacgcaata acgagcggcg
     3601 gtgtgatgca cgcggcggag acgcctgcca tgaaatattg gttcccttca gcgagaagag
     3661 cagtaccggt gacataagcg gctttattga ccgggccgcc catgtcaaat gcgctcatac
     3721 agccgatgat caagccgaga atgattgggt tcgccccttg aaagtcggct aaaaagccct
     3781 tcatcccctc attaatggcc gtcatcggtt cgacgaggaa aaacatgacc gcaccgatca
     3841 gaaaaatgcc gataaccgga taaagaaaaa ttgcttttag tccttcaagt gactgaggca
     3901 tttttttaag tgcatgcgag aggccgtaaa tgatcagtcc cgccaaaaaa ccggatacga
     3961 tgccgcccag aaaacccgct ccgcccgtgc tggcgatcat gccgccgata aaaccgacaa
     4021 tcaggccggg gcgttttgcc attgaatcgg cgatataggc ggataaaatc ggaaccatca
     4081 gctggaatcc gaatgcgccc gttttattca gcaattcggc aaaagcgttg tagcttggat
     4141 gttcaggatc cgctgaatgg atgccgaata aaaaggaaag tgcaatcaaa acccctccgc
     4201 cgacgacaaa tgggagcatg tgggaaaccc cgttcatcaa atgtttatat attttacggc
     4261 cgatggaagc ggattcgcct gtttttggtt catcctctgt ttttgccgca gtttgtccgc
     4321 ggtaaatcgg cgcttcgccg ttcatcagcg tccgaattaa agattccgca tcccggatgc
     4381 cccgcgcaac cgggacgtca aggacacgtt tgcctgcaaa acgctctgcg cctacatctt
     4441 tgtctgccgc gacaatgacc ccgtcggctt gtttaatgtc ctctggcgat agttcgtttt
     4501 gaacgccgat ttgcccgtgt gtttcgactt tgatttccac acccagtttg gcggcagcct
     4561 gttttaaggc ctcttcagcc ataaatgtat gggcaatccc tgttggacag ccggttgccg
     4621 cgatgatttt gaactttttc ataagaaaac cccctttata aatgagtgat ttgaatttgt
     4681 gaagctgttt gatcgagttt ggacacatca ccgagtcctt tcgagaatgc cgtcaaggaa
     4741 ccggcggcag aggcgtgcct gagtgcttca ggaacggaac agccgagctc ctgttttccg
     4801 acgaatacgg caagcatggc atcgccggca catgctgtat tgacaacttt gcctttagcg
     4861 gcggtcgctt ttaatgttct ttggcggtcc aaataaaggg ctccgtcttt gccgcgggag
     4921 atgagaaccc tttcagcgcc gcggtcgaga agttcctttc cgcaatggat gatttcttct
     4981 tcggacagcg ctttcttccg gctgaaaaat gcggcaagct cctgttcatt cggcttaatc
     5041 aggtaaggat ggtatgaaag acaatctaat aggacgggag aactgatatc caagatcaat
     5101 ttcagtttgt tttcggcggc aatctcggaa agcgaaaggt acatgtcatc agggactcct
     5161 ctcggaagac tgcctgaaat aaataagaag cggcccggcg ggatttgacg gattttgttg
     5221 atcagctcct gttttcgggc ttcagatatg agcggtcctt tgtttacaat cttgaattcc
     5281 cgggttgaat tgataaaaat attgatccgt gagatgcctt ccacttcgac aaagtctgtg
     5341 acgatttttt cattcagcag ttcattttca ataaaacgcc cggtgaatcc gcctgtaaag
     5401 ccaagcgcgg tattgtcaag accaaggcgc ttgagcataa atgaaatatt gacgcctttt
     5461 ccgtttggct gataatcttc atctttagta cggttgacaa catcgggaag cagctcgtcg
     5521 gtttcaacga ataagtcgac tgcggtgttc atcgtgcatg tatagatcat gctgtatccc
     5581 ccctttatat gtggcttcac agacattatg tcacagatga aaggggggat gtttacaggt
     5641 tgtgtaaagg ttttcagcta tgagcggtgc ggatgacata tgagtccagc atgatgcggg
     5701 agaggacttg cagagggagg cgagagcgga cttcataatc gggaaaaaat gattgttccc
     5761 ctttaaaccc gaccagtacg atctctgata atttcgcaag acgcgaatcg atccttgtcg
     5821 ttaatgtaat ggtggaaatg gcttgcaatt tgaaattttg agcggcttca acaagctctt
     5881 ctgtctcgcc gttcaaggaa acaaaaaggc cgaggtcttt tttggccagt gtttttgact
     5941 ttcctctgat aatattcggg tcgctgtgca tctcgcagtt tttgccgagc agctgaagct
     6001 tgatcgtcat ctcggttcca atcatttcag acaatcctct tgcgaagatg taaacttttt
     6061 cggcattgct gattttttga atggcatctt cgatcaaccc gatatcaagc atcttaatcg
     6121 tatccagaac ttctctttca ttctttagca cggcctgttt aatttggctg tcgatatcat
     6181 ccatcagcgg agcgtgtccg aggtggtgtt tttcttttaa tgcatactta aaagaagtgt
     6241 agccgtcata tccgagtttt ttcattaacc tgacaatcgt ggctgttgaa acatttgcat
     6301 cctcgcttaa ttttacaatc gataaagtgg cgatgacgtc taagtgctga taaatgtact
     6361 cgagcaaatg ccgttctgaa tcgctgagag attgatatgt gtcagaagtc atttgaaaca
     6421 cgctgtcttt catgataaaa ccccctttta tagtcatatt gtatcagttt agaaaggggg
     6481 aaggagaaaa aataaacatt tcctacataa gaatatccgg ctcgagggcc ggatgtttca
     6541 agttaagaag ccatttttct gcacgcttcc gcgcatgcaa agcagctttc agcgcaaaat
     6601 tggcagtggt catgcttgtg ctggctgcat tcattgccgc aagcttcgca aacctttgcg
     6661 caaagctggc aaatatcttc ggcatacggg ctgttgcgcg tcatggcttg aattgcgtaa
     6721 ccgcacatct ccgcgcattc ccggtcaaga cggatgcatt cggccatcat cccagcctct
     6781 tcttccatca gacatttgtc aaagcagtgg ttgcaggctt caagacattc tcggcaggct
     6841 ttgatgcatt cctcatatcc agatttcacg aaacacactc ctttttcctc gttttccctt
     6901 cgttgtaccc tgatgagatc tctatgctac tccatcgagc cgtcaattgt ctgattcgtt
     6961 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     7021 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     7081 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     7141 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     7201 aaaagacgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     7261 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     7321 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagcaacaa ttgctcaagc
     7381 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     7441 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     7501 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     7561 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     7621 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     7681 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     7741 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     7801 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     7861 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     7921 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     7981 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     8041 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     8101 agattagcgg atcttacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     8161 gatcttacag tgaggcataa ttatggcttt cattccacca accatcgacg acgttagaca
     8221 ttgctctaac gctttatctg tagaccccgc cgaaaccgac gctgcccgcg ccattgctga
     8281 acactactca aagatatcca atcaggagta ccgcatcacc caagacgacc tggatgatct
     8341 cactgacaca atcgaatatc tcatggccac taaccagcca gactcacaat aaggatctcc
     8401 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     8461 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     8521 gtttatagga tctctaatag gctatgtacg cgtatcaaca aatgaacaaa acactgcttt
     8581 acaacgaaac gctcttgaaa gcgcaggatg tgagctaatt tttgaggaca aggcgagcgg
     8641 caaaaaggct gagcgccctg ggttaaaaaa ggtactccgt aactttccag aggtgatacc
     8701 ctggtcgtat ggaagttaga ccgtcttggg cgcagcatgc gtcacttagt tgtgctggtg
     8761 gaagagctgc gtgacagagg cattaacttc cggagtctca ctgactccat cgacaccagt
     8821 acaccaatgg ggcgcttttt ctttcacgta atgggggcgc tggcagaaat ggaacgtgag
     8881 cttatcgttg aacgtacacg cgctggactt gatgcagctc gcgcagaagg tcgtataggt
     8941 gggcgtcggc ctaaatacca agaagaaaca tggcagcaaa tgcggcgatt gctggagaag
     9001 ggcatccccc gtaagcaggt tgcaatcatc tatgatgtgg ctgtttccac gctttataag
     9061 aagtttccgg cgtcatcatt tcaatcctaa accttggttt aagagaactc ggggatctta
     9121 aagcgatttt actttttctg gctatgaatt ggcctgcttt gtaacacact ccggtctatc
     9181 ccgtagcgcc gggcatatcc tgtcgcaatg tgcaaatctc gcggcaacaa ccagtgaata
     9241 cttcattcac aagcctcacc gcctgatcgc ggcagaaact ggttatagcc aatcaaccgt
     9301 cgttcgtgca ttccgtgaag ctgtaaacaa aggtattctg tctgtagaga ttgttatcgg
     9361 cgatcaccgt gaacgtcgcg ctaacctgta ccggtttaca ccatcctttt tggccttcgc
     9421 acaacaagcc aaaaatgcgc tgatagaaag caaattaaag atttcgtcag cggcaaccaa
     9481 ggttaaagct gttctcgcta agacattggc tttatttaat tttttatcca cacccccatg
     9541 tcaaaatgat accccctccc cctgtcagga tgacgtggca ataaagaata agaagtcaca
     9601 agttaaaaaa acaaaaagat cagtttccgg cggtgccgga acaaccagcc tcaaaaaatt
     9661 gacttcatgg atcgctaagg caaaagcaaa ggctgacaat ctgcggttat ccaaaaaacg
     9721 cactcaaaaa catgagttca agcagaaagt agaggcggct gcgcggaaat atgcttacct
     9781 gaaaaacaag cgttcgcctg atattggcgg gatatcaaac ttcgataacc taccgcattg
     9841 catgacggta aacgaagctc ttaatgcggt tttagccaaa aataaagata acgaacaatg
     9901 gggtataccg gcaggattca gagggtaagg atctgaatct ggtgtgttaa ccgattctac
     9961 gagtagtcat ttgttcattg agtaggaata ttgttgggat ctcgaaagga agcataagtg
    10021 acctgggacg atcacaagaa gaattttgct cgcctggcgc gagatggtgg ttacaccatc
    10081 gcacagtatg ccgccgagtt taatcttaac cctaataccg cacgtcgtta tctccgtgcc
    10141 ttcaaagaag acaccaggac tacggacagc cgcaagccaa ataagccagt caggaagcca
    10201 ctaaaaagca tgatcattga tcactctaat gatcaacatg caggtgatca cattgcggct
    10261 gaaatagcgg aaaaacaaag agttaatgcc gttgtcagtg ccgcagtcga gaatgcgaag
    10321 cgccaaaata agcgcataaa tgatcgttca gatgatcatg acgtgatcac ccgcgcccac
    10381 cggaccttac gtgatcgcct ggagcgcgac accctggatg atgatggtga acgctttgag
    10441 ttcgaagttg gcgattacct gatagataac gttgaagcgc ggaaggccgc gcgcgctatg
    10501 ttgcgtcggt ccggggccga tgttctggaa accactcttc tggaaaagtc tctttctcat
    10561 ctccttatgc tggagaacgc cagggatacg tgtattcgcc tggtgcagga aatgcgcgat
    10621 cagcaaaaag acgatgatga aggtactccg cctgaatacc gtatcgcgag catgctaaac
    10681 agctgttccg cgcagataag cagcctgatc aacaccattt acagcatccg gaataactat
    10741 cgaaaagaaa gccgggaggc ggaaaagcac gctttatcta tggggcaagc tggcattgtt
    10801 aagctggcat acgaacgaaa gcgtgaaaat aactggtcag tgctggaagc ggctgagttc
    10861 atcgaggcgc atggaggaaa agtgccgccc ctgatgctgg agcaaatcaa agccgatctg
    10921 cgtgctccta agaccaatac cgatgatgag gaaaaccaaa cagcatctgg cgctccatca
    10981 cttgaggatc tggataaaat cacgcgagaa cgggccgcca gccgccgcgc tgatgccgca
    11041 ttgtggattg agcatcgtag agaagaaatt gccgatatcg tcgatacagg tggttatggt
    11101 gatgtcgatg cggaaggcat atcaaacgaa gcatggcttg aacaggatct ggacgaagac
    11161 gaggaggaag acgaagaagt tacccgcaaa ctgtacgggg atgatgatta aggatctggc
    11221 gattgctggc ctagatgaat gactgtccac gacagaaccc ggcttatcgg tcagtttcac
    11281 ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa
    11341 tgactgtcca cgacgctata cccaaaagaa agcggcttat cggtcagttt cacctggttt
    11401 acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt
    11461 ccacgacact atacccaaaa gaaagcggct tatcggtcag tttcacctgt tttacgtaaa
    11521 aacccgcttc ggcgggtttt tacttttgga ggggcagaaa gatgaatgac tgtccacgac
    11581 actataccca aaagaaagcg gcttatcggt cagttttacc tgatgtacgt aataaaccgt
    11641 tcggatctca attcgaccca gctttcttgt acaaagtggt tgatccttac agatcccggt
    11701 tatccacaga atcaggggag gcctctagaa atattttatc tgattaataa gatgatcttc
//
