LOCUS       sequence-482807-       12793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  coi(Y35H) phagemid with higher infection rate (unpublished).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Al'Abri IS, Haller DJ, Li Z, Crook N
  TITLE     Inducible directed evolution of complex phenotypes in bacteria.
  JOURNAL   Nucleic Acids Res. 2022 Jun 10;50(10):e58. doi: 10.1093/nar/gkac094.
  PUBMED    35150576
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2022-06-10"; volume: "50(10):e58. doi"; pages: "
            10.1093/nar/gkac094"
COMMENT     Sequence Label: ISA894: vioABCDE phagemid
FEATURES             Location/Qualifiers
     source          1..12793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             941..1600
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1007..1026)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1621..1715
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             2314..2322
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             3627..3635
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6647..6655
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             7921..7929
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(7999..8877)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        8904..9188
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     9111..9130
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      9440..9511
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9527..9554
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    complement(12689..12713)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagcc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacactct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgcg gatctttgat
      841 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      901 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      961 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1021 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1081 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1141 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1201 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1261 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1321 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1381 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1441 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1501 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1561 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     1621 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1681 tcggttgccg ccgggcgttt tttattggtg agaatccaag cctcggatcc ttagcgcttg
     1741 gccgcgaaaa ccgcgtccgg gatctccgcc tcgctcacat tcggaaaatc acgcaggctt
     1801 gcacgcgacg cttcatcgcc ggtgaccatg cgcagtggag tgccgctcgc cgcatccagg
     1861 tacagggtgc tcggaccttt acctgggcgc tggtaacgcc aaccgtccgc ttcgcgaccc
     1921 agaaccacgc gacggccaat gtgacgggca cccagacggc gcaggacatc gcgtggcagg
     1981 aacaattcgg caaaagggcc agtctcatca tccagcggac gctcacacag ctgttcaccc
     2041 agggcggtac gctcacgacc ataggcgact ttttgtttcc aggtacggcc gctggccgca
     2101 ttaccaacct ccgacatcca caggcgataa ccagtatcac gctcgctcca cggattgaac
     2161 aggccgtcaa tacgacagat gtcacgttca tagtcgaacc agcaatagcc gctggtcagc
     2221 tggtcatccg gcagcatcgg gctccaataa gagacatagg cgctgctcca acgggctggc
     2281 aacagtggtg gctcacggtt ctccattttt tttacctcct taaagatcat caattcacgc
     2341 gaccaatctt gtaccaaaca ttctgtttac gctggatttg ttgcagacga tcgtacggct
     2401 cgctcgtaaa gtacagtttc tcggccatat ccggcggata caggcccgga aacttggaat
     2461 gcatgtaacg cgtgtaacgc gcacgcatga aaaagatcgg gttcgagcag ctcaaaacgt
     2521 catagttggc gcgagccatg tcttgcattg cgtctgcctg gactttgcgc agctccgtga
     2581 actccggaaa ggctttgtct tggtcgccct ggtggcggtc cagcagctcg acaaacgtgc
     2641 gggcgtcctc cagcgccatg ttcataccct gacccaggaa cggcgcagtc gcatgcgcag
     2701 catcacccag caacagcaca ttacccttat agtgaaaggt gctagagcgc acgttaatca
     2761 ggtcgttgct cggcttcgcc agaaactgac gcagcatttc gtcacgcgcg tcacgcggca
     2821 ggccaccgaa gtaacgatcg aagaacgcac gcatcgtcgg ttcgtcggtg gtcgtcaggg
     2881 aagggctacc cgagtacggc aggcaaacgg cgatgctgac gctaccatct gggatcgtag
     2941 ccgcacgacc cgcgaacagg ccaccggaat ccatgccgaa aaagtacagc gtgtctttac
     3001 ggtaacccag tgcttgcgcg tccggcaaaa ccagggtttt gtagccatgg cggaagaacg
     3061 tttgctggaa ctcgaaacga cgcaggccgc tctgcatcgc ctgacggacg gcgctgtggg
     3121 cgccatccgc accgataatc atgtcacctt gcagacgctg cggctgacca tctttgccct
     3181 gaatcaacac gctcttacca tccaggtcaa catccaggca cttatgctca aagtagtaac
     3241 gaacgcctgc caggttcgcg tatttgttca gcagcttttg aaacgccgca cggttcaagc
     3301 tcagcggacg gaaatcctcc aacggcttca gttcgcgcat gcgatactga ccacccacgg
     3361 agaaagccat tgccacgatc ggttcgccac acgcatccag ctctgcacgc ggaatgcccg
     3421 cagccaaaac gctcttgata ccacgaacgg tcatgctcac gccgatcgca cggctgctaa
     3481 caacgtccac gtaagagctc agatcgcgca gaggatcacc acgcttctcc acgacgtgca
     3541 cttcgtagcc acgcttcgcc aggtagatcg cggtcaggcc acccgccagg ccgccaccaa
     3601 cgataatcgc acgtttcatt ttttttacct ccttaaggat cattaggcct cgcggctcag
     3661 tttgccgcac gccaagtcca gcatttggcg acgatagaaa tccagcagct ccatctgcgc
     3721 atccggcagc caacccggtt ccagcatgct cgcctgctcc agcagacgct cgcaacggcg
     3781 tgccagcatg cgacagccca gcgcgtagtc gtcatagcta cgggtgtcaa ccggacccgg
     3841 cagcggcgga ccggccgtgc gaccggcgat gccgcttggc aggttcatca gcgcgcagct
     3901 cagcggacgc aacagaccgg tcatcagatc gattgctgcg ttcatcaggc ggctgcgacg
     3961 caagctaccc agcggtttca cggcgaagtg ctgcgccatc atcgcaaaca tcagctcata
     4021 aacgccttgg tacaatgcca tcagcgcacg cgcacgaccg tctgcgacac gttggcaacc
     4081 cgggctctca tccagaaccg gattacgcaa cgccggcagc gccggttcga acggtgcgct
     4141 ttgagccatg atacgcgcgc tcatttcacg cagacgttga aaatggctat gttcgtagtg
     4201 cgggctgtcc agagcgccac cttcgccctg atcggtcaca aatgcaatac caaacagcgc
     4261 cgagtcgcga tcgaaaactt ccagttgata acccggatgc gcacggttgg tcagctcatt
     4321 caggaacagg tggtgttcac cgccacgctt acctgcctcg ccaccaaaca agtccggcag
     4381 atccaaaaac gcctgacgaa tggcggcata gcagtccgcg atggatttgc ccggtgctgg
     4441 gataaagtgc ggccattcca gacgaacaaa acgtgccagc gtgctttcgc taaacggttc
     4501 cagagcgaac tcggtgtcca ggccaaacgc ctgacgtgcc gcttcgccca tcagcgggac
     4561 acccgcgtag aacggctcgc ccagggccat cagcaggttg ttaacgacca ggtaatgaat
     4621 catttcttca tgagcaattt ccagcagtgc tgcacgaata ccgccatcac ggcgacggtc
     4681 accgctaccg cacgccagtt gcagctgctc ggcggtccac gcaccgtcac gaacacgttg
     4741 ttggccctgt gcatagttcg gaatgctata cgccgcgtac aggtattgca gcatcacaga
     4801 cagctccagg tcgacggctt tacgcagctc tgccgccaac tgggctttag attcaatgcg
     4861 tgccggaccc gctggcggag gtgcttgcag gcgtgcctgg ccttccacgt gggccagata
     4921 cttcaagaac aaacgagctt tcggtgccga cagttcgcgg gtgctcggca tgtaatagga
     4981 cttgttgcgg ttctgcggat cacacatctg ccacatcaga cgtgcgtacg tttcacattt
     5041 gcaacgatca gccagggaaa acaccttgtc gctcatgaat ggatacacca gctcgtaata
     5101 cgccataacg tggcggtaca aaaaggcgta atccacttct tcgacggtcg cgtcatccag
     5161 cgcccaatcg tcaggcagaa cacgcagagg gatcgcctcg gcaccatcgt ccagaacaat
     5221 gcgtgccgga cccggacgca gacccgtcag acgccattcc gcagcgtcgc catcctgacg
     5281 agattcgacg ccgaccaggc ccatgccctc gatacgatgc gggatatccg gacgcgcacg
     5341 cgcttcaccg cgaaagtagc tgcgcagcgc gatgctctca gggaaaaagc gaccgtgacg
     5401 gcgatccggt gcctccaggt acaggttaga cgcgtcgctt tgggtgaccc agtcttgctc
     5461 acgccactcc gccagttcgc tgctcagggt caagctacca cgcggttcgc gcagcagcgg
     5521 caggtccaca ataccgtgat tcgtccaata gtcttggtac agagcctgcg gcacacgtgc
     5581 caacagtgcg ccgtcctcat cacgcagcag cagatcgccc agcggcagtt tggcacccag
     5641 gtccggggtc aggcggtccg gtgcgcttgg ctgcgcggca cgagtgctga acggaatggc
     5701 acacgccaaa ttcagcgcaa cgcgaccacc gttgacgcgc agggtcaggt cacccagacc
     5761 cggttgacgc ggacgcagca gacgaccagc cgggtagctc gccagttcac cacgacgcca
     5821 cagaccgaca acaccgacca tatcgtgaaa aaccgggctg ttcggctgag gcggggtgct
     5881 catattgaac aacgcatatt gcacggtcag acccagaacg tcgtcatcct ccagagccaa
     5941 ttgcagacga cgccaggcct cggaatcaaa cggacccggg tgaaacagaa aatgtgggtg
     6001 atctttcggc acagagaact gaaacaggcg tgccagacca aactcttcat ccaagaagtg
     6061 gccgccacgc tctgcaatgt ggccgccacg cgtccaacgt gcaccatggc tatcatcaat
     6121 gtctgccgta aacagccacg gcgtacccgg accggcacca gccgggctaa tggtgaattg
     6181 gcccgcatag atttgtgcag cgtcacggcg cgtcgggtcg ctgtcgaccc aacgagcacg
     6241 attgaaggtg gtacgcagat aatcattgta gtgaccccac agtgccaaac gagcaccgac
     6301 cagaccgtca ccacgatccg cctcaccgcc atcccattgc acgtggctaa cggttgcgct
     6361 ctcccacgaa aagtggttgt taccggcagc gttgtagccc tcggccaggc tgaacgggcc
     6421 ttccgggtca gcacgaccat ccaagccgaa gcgcggaccc agggagcgca ggtgacggtg
     6481 gaactccgta ggatggcgtg ccaggtcgaa cggctcaccc gccatcgcca cggtattgct
     6541 ggccatatcg atgtggccgt gcggatcgcg gttcgcggtc ggcgcattga cacgggccca
     6601 gccacggaag tggatacgcg ggaaatccag aatgctcatt ttttttacct ccttaatcac
     6661 gcggcgatac gctgcaacag cagacggctt gcctcacggg cgctcagcag accgccctcc
     6721 atccaaccac aatgctccgt gtacgcatcg gagcacgcga tgataccgct gtcgcgatga
     6781 gacagtgcgc tcgggtggtc aatatcagaa tcacggcaaa actcaacgcc gtgcgcccaa
     6841 tacttgtgaa cgtgtgccag cggttgcggg atacgttcac ggacgatacc cagtgcgcta
     6901 gccaaatggg tgcgaatttg ctccaggtaa ccgtcctcgc cctccgcgac acaaccgcgc
     6961 cagtaattcg ccatctcgct atcggtatag aagaacaggt acttatcgcc tttgaaatag
     7021 attttgcgca gcgggttgtc aacaatcagc acctgatcgt ccagtttgta gtccaaccac
     7081 cacggctcac cgtacgtcag aaagccctta aacagcggca ggctgccata gcgcgcaccg
     7141 gaccaggctt ctggaaaatc aacattcaaa cccgccatcg cgctcggcgg aatcgccaga
     7201 atcagatggc gggtacggtg ctccagtttc cagccgtcgt cacctgccag ttgcagcagg
     7261 tagccgtcac cgtcggtacg gacgctcagc agacgataac ccaggctaaa acgcgcacct
     7321 gccgccttaa ccttagcctt gatgccctga atcagaccag caaagcccgt ttccgctgca
     7381 aaccattggt tcgcgtcgtt gtccgtcacg ctctggatct ccgggtgctt acccacaatg
     7441 tcgtaggcca tttctgcgct gatatccggc aggaacagtg cgtcgtaacc catagagcgg
     7501 atcataccaa ccgcgctatc gtgaccttga taacggctga caaactgcaa aaagctctct
     7561 ttaccatgct ctttcagacg cggggacagt tcattcatgg cgcgcttcag cttttgctgc
     7621 acgtgagatt tgaacttcaa ctgggtgaac ggatagactt cgctcttttg gctatagtgc
     7681 tgcattgcgc tttggaaatg cgggtgcaac tgaggggagt agcgacctgc gcccagttca
     7741 atgcttgcct taccatccag cattttgctg cggatacggc caccggcttc ttgctgcatg
     7801 tcaaagatac gcaggctcag accacggcat gccgggctgt ccagcagatg gcttgcgcac
     7861 gtcaaaccag aaataccagc accaacaatg cagatatcgg aagaatgttt catttttttt
     7921 acctccttaa agatctcatg aattcatgag atctctatgc tactccatcg agccgtcaat
     7981 tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca
     8041 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     8101 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     8161 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     8221 taactgctgg cggaaaagac gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     8281 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     8341 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagcaa
     8401 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     8461 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     8521 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     8581 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     8641 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     8701 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     8761 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     8821 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     8881 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     8941 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     9001 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     9061 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     9121 atttttatcc ataagattag cggatcttac ctgacgcttt ttatcgcaac tctctactgt
     9181 ttctccatac ccggatctta cagtgaggca taattatggc tttcattcca ccaaccatcg
     9241 acgacgttag acattgctct aacgctttat ctgtagaccc cgccgaaacc gacgctgccc
     9301 gcgccattgc tgaacaccac tcaaagatat ccaatcagga gtaccgcatc acccaagacg
     9361 acctggatga tctcactgac acaatcgaat atctcatggc cactaaccag ccagactcac
     9421 aataaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     9481 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     9541 tgggcctttc tgcgtttata ggatctctaa taggctatgt acgcgtatca acaaatgaac
     9601 aaaacactgc tttacaacga aacgctcttg aaagcgcagg atgtgagcta atttttgagg
     9661 acaaggcgag cggcaaaaag gctgagcgcc ctgggttaaa aaaggtactc cgtaactttc
     9721 cagaggtgat accctggtcg tatggaagtt agaccgtctt gggcgcagca tgcgtcactt
     9781 agttgtgctg gtggaagagc tgcgtgacag aggcattaac ttccggagtc tcactgactc
     9841 catcgacacc agtacaccaa tggggcgctt tttctttcac gtaatggggg cgctggcaga
     9901 aatggaacgt gagcttatcg ttgaacgtac acgcgctgga cttgatgcag ctcgcgcaga
     9961 aggtcgtata ggtgggcgtc ggcctaaata ccaagaagaa acatggcagc aaatgcggcg
    10021 attgctggag aagggcatcc cccgtaagca ggttgcaatc atctatgatg tggctgtttc
    10081 cacgctttat aagaagtttc cggcgtcatc atttcaatcc taaaccttgg tttaagagaa
    10141 ctcggggatc ttaaagcgat tttacttttt ctggctatga attggcctgc tttgtaacac
    10201 actccggtct atcccgtagc gccgggcata tcctgtcgca atgtgcaaat ctcgcggcaa
    10261 caaccagtga atacttcatt cacaagcctc accgcctgat cgcggcagaa actggttata
    10321 gccaatcaac cgtcgttcgt gcattccgtg aagctgtaaa caaaggtatt ctgtctgtag
    10381 agattgttat cggcgatcac cgtgaacgtc gcgctaacct gtaccggttt acaccatcct
    10441 ttttggcctt cgcacaacaa gccaaaaatg cgctgataga aagcaaatta aagatttcgt
    10501 cagcggcaac caaggttaaa gctgttctcg ctaagacatt ggctttattt aattttttat
    10561 ccacaccccc atgtcaaaat gataccccct ccccctgtca ggatgacgtg gcaataaaga
    10621 ataagaagtc acaagttaaa aaaacaaaaa gatcagtttc cggcggtgcc ggaacaacca
    10681 gcctcaaaaa attgacttca tggatcgcta aggcaaaagc aaaggctgac aatctgcggt
    10741 tatccaaaaa acgcactcaa aaacatgagt tcaagcagaa agtagaggcg gctgcgcgga
    10801 aatatgctta cctgaaaaac aagcgttcgc ctgatattgg cgggatatca aacttcgata
    10861 acctaccgca ttgcatgacg gtaaacgaag ctcttaatgc ggttttagcc aaaaataaag
    10921 ataacgaaca atggggtata ccggcaggat tcagagggta aggatctgaa tctggtgtgt
    10981 taaccgattc tacgagtagt catttgttca ttgagtagga atattgttgg gatctcgaaa
    11041 ggaagcataa gtgacctggg acgatcacaa gaagaatttt gctcgcctgg cgcgagatgg
    11101 tggttacacc atcgcacagt atgccgccga gtttaatctt aaccctaata ccgcacgtcg
    11161 ttatctccgt gccttcaaag aagacaccag gactacggac agccgcaagc caaataagcc
    11221 agtcaggaag ccactaaaaa gcatgatcat tgatcactct aatgatcaac atgcaggtga
    11281 tcacattgcg gctgaaatag cggaaaaaca aagagttaat gccgttgtca gtgccgcagt
    11341 cgagaatgcg aagcgccaaa ataagcgcat aaatgatcgt tcagatgatc atgacgtgat
    11401 cacccgcgcc caccggacct tacgtgatcg cctggagcgc gacaccctgg atgatgatgg
    11461 tgaacgcttt gagttcgaag ttggcgatta cctgatagat aacgttgaag cgcggaaggc
    11521 cgcgcgcgct atgttgcgtc ggtccggggc cgatgttctg gaaaccactc ttctggaaaa
    11581 gtctctttct catctcctta tgctggagaa cgccagggat acgtgtattc gcctggtgca
    11641 ggaaatgcgc gatcagcaaa aagacgatga tgaaggtact ccgcctgaat accgtatcgc
    11701 gagcatgcta aacagctgtt ccgcgcagat aagcagcctg atcaacacca tttacagcat
    11761 ccggaataac tatcgaaaag aaagccggga ggcggaaaag cacgctttat ctatggggca
    11821 agctggcatt gttaagctgg catacgaacg aaagcgtgaa aataactggt cagtgctgga
    11881 agcggctgag ttcatcgagg cgcatggagg aaaagtgccg cccctgatgc tggagcaaat
    11941 caaagccgat ctgcgtgctc ctaagaccaa taccgatgat gaggaaaacc aaacagcatc
    12001 tggcgctcca tcacttgagg atctggataa aatcacgcga gaacgggccg ccagccgccg
    12061 cgctgatgcc gcattgtgga ttgagcatcg tagagaagaa attgccgata tcgtcgatac
    12121 aggtggttat ggtgatgtcg atgcggaagg catatcaaac gaagcatggc ttgaacagga
    12181 tctggacgaa gacgaggagg aagacgaaga agttacccgc aaactgtacg gggatgatga
    12241 ttaaggatct ggcgattgct ggcctagatg aatgactgtc cacgacagaa cccggcttat
    12301 cggtcagttt cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg
    12361 gcagaaagat gaatgactgt ccacgacgct atacccaaaa gaaagcggct tatcggtcag
    12421 tttcacctgg tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga ggggcagaaa
    12481 gatgaatgac tgtccacgac actataccca aaagaaagcg gcttatcggt cagtttcacc
    12541 tgttttacgt aaaaacccgc ttcggcgggt ttttactttt ggaggggcag aaagatgaat
    12601 gactgtccac gacactatac ccaaaagaaa gcggcttatc ggtcagtttt acctgatgta
    12661 cgtaataaac cgttcggatc tcaattcgac ccagctttct tgtacaaagt ggttgatcct
    12721 tacagatccc ggttatccac agaatcaggg gaggcctcta gaaatatttt atctgattaa
    12781 taagatgatc ttc
//
