LOCUS       sequence-48284-e        7485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: RAD Module G8-C1
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(78..121)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    124..145
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    146..164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    171..189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             234..1733
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1761..1788
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1871..2749)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2776..3059
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2982..3001
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3962..5458
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     misc_feature    5504..5524
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5525..5582
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5606..5623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5833..6421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5913..5932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6509..6603)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6624..7283)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7198..7217
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7284..7386)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctggaattcg cggccgcttc tagagtccct atcagtgata gagattgaca tccctatcag
      181 tgatagagat actgagcaca agcttgcggc tttcacactt gggggctagc gtgagagccc
      241 tggtagtcat ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg
      301 agtcttgcca gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc
      361 tggacgtctc cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt
      421 ggctagcgtt cgaggagcaa ccgtttgacg tgatcgtggc gtaccgggta gatcggttga
      481 cccgatcgat ccggcatctt cagcagctgg tccactgggc cgaggaccac aagaagctgg
      541 tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg
      601 cgcttatggg aacggtggcg cagatggaat tagaagcgat caaagagcgg aaccgttcgg
      661 ctgcgcattt caatatccgc gccgggaaat accgaggatc cctgccgccg tggggatacc
      721 tgcctacgcg cgtggacggg gagtggcggc tggtgccgga ccctgtgcag cgagagcgca
      781 tcctcgaggt gtatcaccgc gtcgtcgaca accacgagcc gctgcatctg gtggcccacg
      841 acctgaaccg gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg caaggccgcg
      901 agccgcaggg ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc tccgaggcga
      961 tgctcgggta cgcgactctg aacggtaaga ccgtccgaga cgacgacgga gccccgctgg
     1021 tgcgggctga gccgatcctg acccgtgagc agctggaggc gctgcgcgcc gagctcgtga
     1081 agacctcccg ggcgaagccc gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct
     1141 gcgcggtgtg cggggagccc gcgtacaagt tcgccggggg aggacgtaag cacccgcgct
     1201 accgctgccg ctcgatgggg ttcccgaagc actgcgggaa cggcacggtg gcgatggccg
     1261 agtgggacgc gttctgcgag gagcaggtac tggatctgct cggggacgcg gagcgtctgg
     1321 agaaagtctg ggtagcgggc tcggactccg cggtcgaact cgcggaggtg aacgcggagc
     1381 tggtggacct gacgtcgctg atcggctccc cggcctaccg ggcgggctct ccgcagcgag
     1441 aagcactgga tgcccgtatt gcggcgctgg ccgcgcggca agaggagctg gagggcctgg
     1501 aggctcgccc gtctggctgg gagtggcgcg agaccgggca gcggttcggg gactggtggc
     1561 gggagcagga caccgcggca aagaacacct ggcttcggtc gatgaacgtt cggctgacgt
     1621 tcgacgtccg cggcgggctg actcgcacga tcgacttcgg ggatcttcag gagtacgagc
     1681 agcatctcag gctcggcagc gtggtcgaac ggctacacac cgggatgtcg taagcggccg
     1741 catgcattaa taatcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1801 agagagaata taaaaagcca gattattaat ccggcttttt tattatttcc tgcagggcgg
     1861 ccgccatatg ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     1921 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     1981 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     2041 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     2101 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     2161 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     2221 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     2281 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     2341 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     2401 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     2461 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     2521 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     2581 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     2641 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     2701 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     2761 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     2821 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     2881 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     2941 gaaaagtcac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     3001 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     3061 ccgttttttt gggctagcta ctagacctta acaaactacg gaggtcaaca tggccggcac
     3121 tcagcgtatc gtctttctac ccgatactca gttgcctttc gaggcgcgca aagagatgca
     3181 agcggtcatc cgcttcatcg gggatgtcca gccgtacggc gtggtacata tcggtgacgt
     3241 cctagacctg ccgcagccct cgcgctggaa ccggggaacc aagggcgagt tcgagggctc
     3301 ggtgtaccgc gacgcggact acgccaagaa gaacctgatg gagccgctgc gcaaggtcta
     3361 cgacggctgg atcgggatgc acgagggcaa ccacgatctg cgagcccgcg agtacctggc
     3421 caagaacgca ccggccctgg agggtacgca cgctttcgac atcgacgtgc tgctcgactt
     3481 cgacgggttc ggtgtggagc tgctgcctga cttctacgac atcgctccgg gctggatctc
     3541 cactcacggg cacatgggca agatgacgct atcccagatc gccggatcga cagcgctcaa
     3601 cggtgccaag aagttcggca agtccgtggt ctgcggccac acgcaccggc aggctgtcgt
     3661 ctcgcactcg ttcgggtacg gcggctcggt gcgcaagacc gtcaccggca tggaagtcgg
     3721 gcacctgatg gacatgaaga aggccaacta tctaaagggc ggagccggga actggcagat
     3781 gggcttcggg atgctcacgg tcgacggcaa gcatgtcaag gctgagatcg tcccgatcct
     3841 gggaggcaag ttcaccgttg acggccaggt ctgggaagtc accggtgcag caaacgacga
     3901 aaactacgct gctgctaaat aataaggcgc gcctagagtc acacaggaaa cctactaggt
     3961 gagagccctg gtagtcatcc gcctgtcccg cgtcaccgat gctacgactt caccggagcg
     4021 tcagctggag tcttgccagc agctctgcgc ccagcgcggc tgggacgtcg tcggggtagc
     4081 ggaggatctg gacgtctccg gggcggtcga tccgttcgac cggaagcgca gaccgaacct
     4141 ggcccggtgg ctagcgttcg aggagcaacc gtttgacgtg atcgtggcgt accgggtaga
     4201 tcggttgacc cgatcgatcc ggcatcttca gcagctggtc cactgggccg aggaccacaa
     4261 gaagctggtc gtctccgcga ccgaagcgca cttcgatacg acgacgccgt ttgcggcggt
     4321 cgtcatcgcg cttatgggaa cggtggcgca gatggaatta gaagcgatca aagagcggaa
     4381 ccgttcggct gcgcatttca atatccgcgc cgggaaatac cgaggatccc tgccgccgtg
     4441 gggatacctg cctacgcgcg tggacgggga gtggcggctg gtgccggacc ctgtgcagcg
     4501 agagcgcatc ctcgaggtgt atcaccgcgt cgtcgacaac cacgagccgc tgcatctggt
     4561 ggcccacgac ctgaaccggc gtggtgtcct gtcgccgaag gactacttcg cgcagctgca
     4621 aggccgcgag ccgcagggcc gggagtggtc ggctaccgcg ctgaagcgat cgatgatctc
     4681 cgaggcgatg ctcgggtacg cgactctgaa cggtaagacc gtccgagacg acgacggagc
     4741 cccgctggtg cgggctgagc cgatcctgac ccgtgagcag ctggaggcgc tgcgcgccga
     4801 gctcgtgaag acctcccggg cgaagcccgc ggtgtctacc ccgtcgctgc tgctgcgggt
     4861 gttgttctgc gcggtgtgcg gggagcccgc gtacaagttc gccgggggag gacgtaagca
     4921 cccgcgctac cgctgccgct cgatggggtt cccgaagcac tgcgggaacg gcacggtggc
     4981 gatggccgag tgggacgcgt tctgcgagga gcaggtactg gatctgctcg gggacgcgga
     5041 gcgtctggag aaagtctggg tagcgggctc ggactccgcg gtcgaactcg cggaggtgaa
     5101 cgcggagctg gtggacctga cgtcgctgat cggctccccg gcctaccggg cgggctctcc
     5161 gcagcgagaa gcactggatg cccgtattgc ggcgctggcc gcgcggcaag aggagctgga
     5221 gggcctggag gctcgcccgt ctggctggga gtggcgcgag accgggcagc ggttcgggga
     5281 ctggtggcgg gagcaggaca ccgcggcaaa gaacacctgg cttcggtcga tgaacgttcg
     5341 gctgacgttc gacgtccgcg gcgggctgac tcgcacgatc gacttcgggg atcttcagga
     5401 gtacgagcag catctcaggc tcggcagcgt ggtcgaacgg ctacacaccg ggatgtcgac
     5461 cggcgcagca aacgacgaaa actacgctgc tcgtcattaa taatactagt agcggccgct
     5521 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
     5581 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5641 tgcggcgagc ggtatcagct cactcaaagg cggtaatcct agctagggcg ttcggctgcg
     5701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6001 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     6061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6481 agggattttg gtcatgactt gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     6541 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     6601 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     6661 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     6721 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     6781 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     6841 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     6901 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     6961 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     7021 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     7081 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     7141 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     7201 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     7261 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     7321 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     7381 cgatcaacgt ctcattttcg ccagatatcg acgtctaaga aaccattatt atcatgacat
     7441 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacc
//
