LOCUS       sequence-482841-        8552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for mCherry-TEV-mVenus-tagged proteins with 
            FAST-Red transgenic seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Schwartz S, Engstler C, Muhlbauer S, Windenbach E, Wunder T, Kunz 
            HH, Brandt B
  TITLE     
  JOURNAL   Plant J. 2025 Nov;124(3):e70544. doi: 10.1111/tpj.70544.
  PUBMED    41222448
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2025-11"; volume: "124(3):e70544. doi"; pages: " 
            10.1111/tpj.70544"
COMMENT     Sequence Label: pG20_mCherry_TEV_mVenus_FR
FEATURES             Location/Qualifiers
     source          1..8552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             complement(1097..1792)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     protein_bind    3770..3791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3806..3811,3812..3829,3830..3836)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3844..3860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3878..3889
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3896..4573
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4618..4689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4705..4732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4757..5461
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4899..4917)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5471..5491
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(5495..5497,5498..5500,5501..6214)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5540..5561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5801..5820)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6148..6169
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6635..6659
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6750..7338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6830..6849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7509..8324)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7509..8324)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8232..8251
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8316..8335
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcactaaa
      661 tggagcaacc tactgttttt gaagtttggg ttgccatgta atcacttgca ataaatatat
      721 gtactaatga ctgcatcggc ttgcgtgtct aaatttgtag cttattgaaa actttttgtg
      781 gttaaagacg aatccagtta actaacataa gtttaacaaa acaagacctt ttccatatat
      841 agaccaccgt taccaactta caaagaagcc aaataaagta caacacaaca cttgcaagtt
      901 acaaaaagag aggaaaaaaa acatggtacc atttgacaac atatagaaac acacataaac
      961 attacttata acaaaaagat tcaaactcgg cttttattat ctccccctga tcaaattcta
     1021 aacactttat tcctcataaa ctaactcccc gacattggag ttattggact atgacgatga
     1081 catcagtggg gtaagctcac ttgtgcccca gtttgctagg gaggtcgcag tatctggcca
     1141 cagccacctc gtgctgctcg acgtaggttt ctttgtcggc ctccttgatt ctttccagtc
     1201 tgtggtccac atagtagacg ccgggcatct tgaggttctt agcgggtttc ttggatctgt
     1261 atgtggtctt gaagttgcag atcaggtggc ccccgcccac gagcttcagg gccatgtcgc
     1321 ttctgccttc caggccgccg tcagcggggt acagcatctc ggtgttggcc tcccagccga
     1381 gtgttttctt ctgcatcaca gggccgttgg atgggaagtt cacccctctg atcttgacgt
     1441 tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccat
     1501 cttcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc ttaaagaagt
     1561 cggggatgcc ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag ctggtagcca
     1621 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
     1681 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg
     1741 tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcgctc atagtagtgt
     1801 gctggccacc acgagtacgg tcacggtcat gttccccacc agtatgttgc tgtccgtagt
     1861 actgagctct gtctttcaga tcctgagctt tgcttcccaa cttcatcctt gcactgtcca
     1921 acttgtctga tccctgtggg tgctctcccg ttgcgtacct attcacaatc aataggtatc
     1981 ataccaagca ttttacctat atagatgata tacacagaca cattcaagct agctaggtca
     2041 aatttcgtat ttagttaacc aaatgttccc tagaatttgc aatttcttat tgttacattt
     2101 gttattcgac caaaaaaatt gatccaaagc tactggctca acacatgcat gcacatattg
     2161 cacaaaatta tgaagtaaga tgataaatgt gtgcttactt gtaaatccaa gagaaaacgg
     2221 ttatagcggc aatgccaaac cctccagagg aaagaaaacc ggtgatgagg agtgcaactg
     2281 tgatgagagc cgggacaagg attgggctga agataacgag cagaggtgtt gcaacagtca
     2341 aagctatgac agttccaaca agggtaaggc tggagagaac aaggagggaa ccaccagctg
     2401 tgacagcagt tgcagcttta gcaatctgcc tagacttgga gtagtcagat cctcgtccgg
     2461 acatctggta ctggtcacga tctcggccca tcatcgggta ctgatctctg ccgatgatat
     2521 cgtgatgggt tcctctagct gtatccgcca tttttttgtt cttgtttact agagagaatg
     2581 agattttgaa tgagagattg atcaaaaaga tgtgtatata ttttggaaga ggtaggagtg
     2641 tttgttagat atattaagaa aggagacgag aagtcacgtg tcaatggaga catcatgcaa
     2701 ttgtaggcca cgtgttaatg gcgtcaccat gcagccgctg catgtgttaa cgagaggagt
     2761 ctattcaaag acacgtttgt tttttgccta gctcagaatg tggcgttggc atcgaaccga
     2821 aacataaaac gcaaaacgaa cctttgggct cgtcagtatg ggtttgggcc ttcaatagat
     2881 tccttctata cggcccacat atccactatt ggcaacaaaa ttagaaaaat ttaggattta
     2941 gttttaaaat ttgttccgcg acattataaa gcttcgacga gtcagtaata aacggcgtca
     3001 aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc
     3061 ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata
     3121 cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg
     3181 tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagacctct tcttcttcac
     3241 aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat
     3301 caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc
     3361 ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa gtcgattttc
     3421 tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga
     3481 tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta
     3541 gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg
     3601 attaacagac tagagttaac cgggctcagg cctggcgcgc cactagtgga tccgatggtg
     3661 tcttcgattc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3721 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3781 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     3841 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatggc
     3901 ttcctccgag gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     3961 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     4021 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     4081 cccgcagttc cagtacggtt ccaaggctta cgttaaacac ccggctgaca tcccggacta
     4141 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaggacgg
     4201 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     4261 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaacgatggg
     4321 ttgggaagca tccaccgaac gtatgtaccc ggaggacggt gctctgaaag gtgaaatcaa
     4381 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     4441 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     4501 ctcccacaac gaggactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     4561 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     4621 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     4681 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataga
     4741 agacactgga cccggggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     4801 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     4861 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     4921 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     4981 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     5041 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     5101 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     5161 cgacggcccc gtaatgcaga agaaaacgat gggctgggag gcctcctccg agcggatgta
     5221 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     5281 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     5341 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     5401 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     5461 gtccgggagt gagaacctct acttccaagg ctccatggtg agcaagggcg aggagctgtt
     5521 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5581 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
     5641 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
     5701 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5761 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5821 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5881 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5941 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     6001 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     6061 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     6121 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     6181 gatcactctc ggcatggacg agctgtacaa gtaagagctc atatgaagat gaagatgaaa
     6241 tatttggtgt gtcaaataaa tagcttgtgt gcttaagttt gtgttttttt cttggcttgt
     6301 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatctc attataatga
     6361 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat
     6421 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag gaattcagat
     6481 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc
     6541 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg
     6601 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaact
     6661 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     6721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7201 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     7261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     7501 tctagtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     7561 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     7621 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     7681 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     7741 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     7801 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     7861 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     7921 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     7981 tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac
     8041 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     8101 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     8161 ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat
     8221 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     8281 aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     8341 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     8401 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     8461 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     8521 ttcaaaatca ccaactggtc cacctacaac aa
//
