LOCUS       sequence-483022-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Displays Fc-fusion hST6Gal1 WT on mammalian cell surface via 
            transmembrane domain of DPP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Hombu R, Neelamegham S
  TITLE     Mammalian Cell Surface Display for High-Throughput Protein 
            Engineering of Glycosyltransferases.
  JOURNAL   Small. 2025 May 26:e2502318. doi: 10.1002/smll.202502318.
  PUBMED    40417887
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small. 2025
            May 26:e2502318. doi: 10.1002/smll.202502318."
COMMENT     Sequence Label: pCSCG-TM(DPP4)-Fc-hST6Gal1 WT
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4768..4788
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4789..4806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             5319..5499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5525..5542)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5531..5755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5801..6229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5888..5907)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6098..6119
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6238..6258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6243..6572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6423..6558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6485..6504
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6620..6667
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6686..7060
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7190..7311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7227..7246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7281..7300
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7360..7376)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7360..7376)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7373..7395)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7384..7400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7408..7414,7415..7432,7433..7438))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7453..7474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7591..7608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7762..8350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7842..7861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8521..9312,9313..9381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9144..9163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9382..9486)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3001 cgccaccatg aagacaccgt ggaagaccgg tgttcttctg ggactgctgg gtgctgctgc
     3061 gcttgtcacc atcatcaccg tgcccgtggt tctgctgtct agaacatgcc caccgtgccc
     3121 agcacctgaa ctcgcggggg caccgtcagt cttcctcttc cccccaaaac ccaaggacac
     3181 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     3241 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     3301 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     3361 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     3421 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac
     3481 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa
     3541 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     3601 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct
     3661 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     3721 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aaggtagcgg
     3781 ctctggtagc ggttccggct ctgaggcctc cttccaggtg tggaacaagg acagctcttc
     3841 caaaaacctt atccctaggc tgcaaaagat ctggaagaat tacctaagca tgaacaagta
     3901 caaagtgtcc tacaaggggc caggaccagg catcaagttc agtgcagagg ccctgcgctg
     3961 ccacctccgg gaccatgtga atgtatccat ggtagaggtc acagattttc ccttcaatac
     4021 ctctgaatgg gagggttatc tgcccaagga gagcattagg accaaggctg ggccttgggg
     4081 caggtgtgct gttgtgtcgt cagcgggatc tctgaagtcc tcccaactag gcagagaaat
     4141 cgatgatcat gacgcagtcc tgaggtttaa tggggcaccc acagccaact tccaacaaga
     4201 tgtgggcaca aaaactacca ttcgcctgat gaactctcag ttggttacca cagagaagcg
     4261 cttcctcaaa gacagtttgt acaatgaagg aatcctaatt gtatgggacc catctgtata
     4321 ccactcagat atcccaaagt ggtaccagaa tccggattat aatttcttta ataactacaa
     4381 gacttatcgt aagctgcacc ccaatcagcc cttttacatc ctcaagcccc agatgccttg
     4441 ggagctatgg gacattcttc aagaaatctc cccagaagag attcagccaa accccccatc
     4501 ctctgggatg cttggtatca tcatcatgat gacgctgtgt gaccaggtgg atatttatga
     4561 gttcctccca tccaagcgca agactgacgt gtgctactac taccagaagt tcttcgatag
     4621 tgcctgcacg atgggtgcct accacccgct gctctatgag aagaatttgg tgaagcatct
     4681 caaccagggc acagatgagg acatctacct gcttggaaaa gccacactgc ctggcttccg
     4741 gaccattcac tgctgacctg caggttcgaa aacctgtact tccagggcca tcaccatcac
     4801 catcactgac tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     4861 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     4921 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4981 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5041 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5101 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5161 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5221 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5281 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5341 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5401 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5461 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     5521 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5581 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5641 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5701 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5761 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5821 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5881 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5941 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6001 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6061 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6121 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6181 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6241 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6361 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6481 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6541 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6601 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     6661 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     6721 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     6781 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     6841 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     6901 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     6961 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7021 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7081 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7141 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7321 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7381 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7441 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7501 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7561 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8161 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8281 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8341 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8401 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8461 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8521 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8581 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8641 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8701 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8761 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8821 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8881 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8941 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9001 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9061 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9121 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9181 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9241 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9301 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9361 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9421 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9481 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
