LOCUS       sequence-483092-        9965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CCP expression in diverse gram-negative bacteria, with pre-cloned 
            Golden Gate Compatible (BsaI) restriction sites to facilitate 
            promoter swapping..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 9922 to 9956)
  AUTHORS   Armstrong ZR, Alonso J, Stanton V, Patel N, Zogaj X, Cocioba SS, 
            Klose KE
  TITLE     Mobilizable shuttle vectors with fluorescent markers functional 
            across different species of bacteria.
  JOURNAL   Appl Environ Microbiol. 2025 May 12:e0004525. doi: 
            10.1128/aem.00045-25.
  PUBMED    40353662
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2025 May 12:e0004525. doi: 10.1128/aem.00045-25."
COMMENT     Sequence Label: pKEK3394
FEATURES             Location/Qualifiers
     source          1..9965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             4..15
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(2102..2118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2102..2118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2310..2987
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     ADPA"
     primer_bind     complement(2376..2395)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3441..3835
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             5380..6351
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6862..7701
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7688..8539
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     complement(9166..9183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9300..9845)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9380..9399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9899..9915
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9922..9956
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 attaaagagg agaaagagtc ccatggcgag cttagtcaag aaagacatgt gtgtaaagat
       61 gacgatggag gggactgtta atggttacca ctttaagtgt gtgggggaag gtgaggggaa
      121 accgtttgag ggaactcaaa acatgcgtat tcgcgtcacg gagggtggac ctttaccgtt
      181 cgctttcgat attctggcac catgttgcat gtatgggagc aaaaccctga tgaatcccct
      241 aatgattttt atcaaaatca ttaagttaag gtagatacac atcttgtcat atgatcaaat
      301 ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg aactcgatat tttacacgac
      361 tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct
      421 tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca
      481 accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac
      541 ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat
      601 acgatgccca ttgtacttgt tgactggtct gatatccgtg agcaaaaacg gcttatggta
      661 ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg
      721 ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt
      781 ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat
      841 aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat
      901 gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt
      961 cactcaaaga ctttaggcta taagaggctg actaaaagca acccaatctc atgccaaatt
     1021 ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt
     1081 caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatggat tctagcaact
     1141 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
     1201 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
     1261 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
     1321 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
     1381 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
     1441 cggcattctg gctacacaat aacaagggaa gactcactcg tggctgcaac cctgcttact
     1501 caaaatctat tcacacatgg ttacgttttg gggaaattat gaggggatct ctcagagcaa
     1561 aacctttatt aaacatgtca gcggcatccc agactatttt aaagagtcgt tccccgaagg
     1621 gttcacctgg gagcgtacac agatcttcga ggatggcgga gtattgaccg cacatcagga
     1681 tacaagtctt gagggcaatt gcttgattta caaagtgaaa gtgcttggga cgaatttccc
     1741 tgccaacgga cccgttatgc aaaagaaaac tgcaggctgg gagccgtgtg ttgagatgct
     1801 ttacccacgt gacggggtat tgtgcggcca aagtctgatg gcactgaaat gtaccgatgg
     1861 taatcactta acgtcgcact tacgtacaac ataccgtagc cgtaagccct ccaacgcggt
     1921 caacatgcct gagtttcact ttggcgacca ccgcattgag atcttaaagg cggaacaggg
     1981 gaaattttat gaacagtatg agtcggctgt ggcccgttac tctgatgtgc ctgagaaagc
     2041 aacttaatga gagctcaaaa aaaaaccccg cccctgacag ggcggggttt tttttggtac
     2101 cgtcatagct gtttcctgct ccccaacggc gtagaggatc gagatctttt gggttgtcac
     2161 tcatcgtatt tggtttataa ttttaagcta ataacctaat tataactaat taatagtttt
     2221 gtatcttgaa aaaatagcta taaaacttat ttaaataacg aagatttttg tgtataaaat
     2281 atttataaca aaaaaaggag actaaacata tggagaaaaa aatcactgga tataccaccg
     2341 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     2401 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     2461 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     2521 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     2581 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     2641 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     2701 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     2761 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     2821 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     2881 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     2941 actgcgatga gtggcagggg gggcgtaaac gccgcgcgga tccggctgct aacaaagcgc
     3001 gagcctgtca gaccaagttt actcatatat actttagatt gatttctgaa agcgaccagg
     3061 tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat
     3121 tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
     3181 ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
     3241 ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
     3301 tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
     3361 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
     3421 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
     3481 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
     3541 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
     3601 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
     3661 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
     3721 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
     3781 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
     3841 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
     3901 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
     3961 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
     4021 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc acccgctgaa tttcggcatt
     4081 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
     4141 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg agccgaaaag
     4201 caaaagcaac agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg
     4261 tcgggcggcc aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc
     4321 cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg
     4381 cccgccgact actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag
     4441 gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc
     4501 gagttcgccc agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc
     4561 ggtggcggcg agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc
     4621 gagcggcccg ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg
     4681 gcacagaaga ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg
     4741 gccgaccatg ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga
     4801 acacttgagg cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg
     4861 gagatggaag gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc
     4921 gccaacgccc agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat
     4981 cgacagagtg aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc
     5041 ccagagcatg gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc
     5101 cagcgaggcg caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt
     5161 ggagctggcc aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc
     5221 ccggagcgcg tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat
     5281 gatgcctacg gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac
     5341 aaccgaggac ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg
     5401 caggccatag gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg
     5461 gacgccacca ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac
     5521 acgccatggc tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag
     5581 caggagcggc atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg
     5641 aaagccgagg gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca
     5701 tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg
     5761 gccagcgagt acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg
     5821 ggcttcacca accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg
     5881 ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc
     5941 cagcagatcg agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa
     6001 ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag
     6061 atggccgggc tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg
     6121 cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc
     6181 agcccagcgc tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc
     6241 agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg
     6301 gcaccccgcc agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgcttgcgt
     6361 tggtactcac gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac
     6421 gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc
     6481 ggtggctttg gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg
     6541 gccagcctta accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc
     6601 cccggcaagt ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct
     6661 gctggccagc cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg
     6721 gatgcgtcag cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct
     6781 ggaagaactc agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac
     6841 tgcctgcaaa ggaggcaatc aatggctacc cataagccta tcaatattct ggaggcgttc
     6901 gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca tggtggccgg tacggtcggg
     6961 gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg ccctgcaact ggccgcacag
     7021 attgcaggcg ggccggatct gctggaggtg ggcgaactgc ccaccggccc ggtgatctac
     7081 ctgcccgccg aagacccgcc caccgccatt catcaccgcc tgcacgccct tggggcgcac
     7141 ctcagcgccg aggaacggca agccgtggct gacggcctgc tgatccagcc gctgatcggc
     7201 agcctgccca acatcatggc cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc
     7261 cgccgcctga tggtgctgga cacgctgcgc cggttccaca tcgaggaaga aaacgccagc
     7321 ggccccatgg cccaggtcat cggtcgcatg gaggccatcg ccgccgatac cgggtgctct
     7381 atcgtgttcc tgcaccatgc cagcaagggc gcggccatga tgggcgcagg cgaccagcag
     7441 caggccagcc ggggcagctc ggtactggtc gataacatcc gctggcagtc ctacctgtcg
     7501 agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc
     7561 gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg
     7621 cggcatgacg gcggggtgct caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg
     7681 gtgccccgtg gtgaagccta agaacaagca cagcctcagc cacgtccggc acgacccggc
     7741 gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa
     7801 gctggacgtg acgtatgact acggcgacgg caagcggatc gagttcagcg gcccggagcc
     7861 gctgggcgct gatgatctgc gcatcctgca agggctggtg gccatggctg ggcctaatgg
     7921 cctagtgctt ggcccggaac ccaagaccga aggcggacgg cagctccggc tgttcctgga
     7981 acccaagtgg gaggccgtca ccgctgatgc catggtggtc aaaggtagct atcgggcgct
     8041 ggcaaaggaa atcggggcag aggtcgatag tggtggggcg ctcaagcaca tacaggactg
     8101 catcgagcgc ctttggaagg tatccatcat cgcccagaat ggccgcaagc ggcaggggtt
     8161 tcggctgctg tcggagtacg ccagcgacga ggcggacggg cgcctgtacg tggccctgaa
     8221 ccccttgatc gcgcaggccg tcatgggtgg cggccagcat gtgcgcatca gcatggacga
     8281 ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac cagcggctgt gtggctggat
     8341 cgaccccggc aaaaccggca aggcttccat agataccttg tgcggctatg tctggccgtc
     8401 agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg gtgcgcgagg cgttgccgga
     8461 gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg ggcaagtacg acatcacccg
     8521 gcccaaggcg gcaggctgac cccccccact ctattgtaaa caagacattt ttatctttta
     8581 tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca tgctcagaaa
     8641 aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct ccataggccg
     8701 ctttcctggc tttgcttcca gatgtatgct cttctgctcc cgaacgccag caagacgtag
     8761 cccagcgcgt cggccagctt gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact
     8821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8881 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
     8941 gcaacagctg attgcccttc acctccaagc tgtccctcct gttcagctac tgacggggtg
     9001 gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact
     9061 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     9121 accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     9181 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     9241 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     9301 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     9361 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     9421 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     9481 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     9541 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9601 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     9661 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     9721 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     9781 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     9841 ctcaagaaga tcatcttatt aatcagataa aatatttcta gagtagtcga ctgagaccgt
     9901 aaaacgacgg ccagtgaatt cttgacggct agctcagtcc taggtacagt gctagcttgg
     9961 tctcg
//
