LOCUS       sequence-48311-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED30
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             94..138
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             169..183
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             751..774
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atcctcaatc ggaggttcac tagtggttct ggtcatcacc atcaccatca ctccgcgggt
       61 ctggtgccac gcggtagtac tgcaattggt atgaaagaaa ccgctgctgc taaattcgaa
      121 cgccagcaca tggacagccc agatctgggt accggtggtg gctccggtga tgacgacgac
      181 aagagtccca tgggatatcg gggatccatg tccacccctc cgctggcgcc cacgggcatg
      241 gcgtccgggc ccttcggcgg cccgcaggct cagcaggccg cgcgcgaggt caacacggcc
      301 acgctgtgcc gcatcgggca ggagaccgtg caggacatcg tgtaccgcac catggagatc
      361 ttccagctgc tcaggaacat gcagctgcca aatggtgtca cttaccatac tggaacttac
      421 caagaccggc taacaaagct gcaggaccac cttcggcaac tttctattct cttcaggaag
      481 ctgcgactgg tctatgacaa atgtaatgag aactgtggtg ggatggaccc cattcctgtt
      541 gagcaactga ttccatatgt ggatgaagat ggctcaaaga acgacgaccg ggctggtcca
      601 cctcgttttg ctagcgaaga gagacgagaa attgtagaag taaataagaa actcaaacag
      661 aagaatcaac agctgaagca gattatggat caattacgga atctcatctg ggacataaat
      721 gccatgctgg caatgaggaa ctaactcgag caccaccacc accaccacca ccactaattg
      781 attaatacct aggctgctaa acaaagcccg aaaggaagct gagttggctg ctgcacgtga
      841 catata
//
