LOCUS       sequence-48312-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower 
            CS, Conaway RC, Conaway JW
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional 
            coactivator intersex is a subunit of the mammalian Mediator complex.
  JOURNAL   J Biol Chem. 2003 Dec 12. 278(50):49671-4.
  PUBMED    14576168
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Dec 12. 278(50):49671-4."
COMMENT     Sequence Label: pET19b-MED29
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..83
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             99..113
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggaaattccc tctgaatatt ttgtttactt taagaaggag atataccatg ggccatcatc
       61 atcatcatca tcatcatcat cacagcagcg gccatatcga cgacgacgac aagcatatgc
      121 tcgaggctgc tggtgtatcg ggtcctagtt cggctggcgg cccgggtccc cagcagcagc
      181 cgcaaccgcc agcacaactg gtgggccctg cccagagcgg cctcctgcag caacagcaac
      241 aggacttcga tcctgtgcag cgttataaga tgctcatccc gcagctgaag gagagtctac
      301 agaccttgat gaaggttgcg gcccaaaact tgattcagaa cactaacatc gacaatggac
      361 aaaagagcag tgatggaccc atacagcgct ttgacaagtg cctggaagag ttctatgcac
      421 tctgtgacca gctggagctg tgcctgcgcc tggcgcatga gtgcctgtca cagagttgtg
      481 acagtgccaa gcactctcca acgttggtgc ccacagccac caagcccgac gcagtgcagc
      541 ctgacagcct cccctaccca cagtacctgg cggtcatcaa agcccagatt tcctgtgcca
      601 aggacattca caccgccctg ctggactgtg ccaacaaggt cacgggcaag acacccgcac
      661 cacctgctgg ccctgggggc actctgtgag gatcctgaac gaaaaaggaa gaagaaagaa
      721 aagaagaaga agaagaaccg acatagtcca gaccacccag gtatgggcag ctctcaagcc
      781 agcagcagca gcagcctccg ctaaagatcc ggctgctaca agcgaagagt gatgt
//
