LOCUS       sequence-483129-       13219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light-controlled release of dCas9-VPR from the cytoplasm to the
            nucleus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Baloban M, Manoilov KY, Karasev MM, Verkhusha VV, Shcherbakova DM
  TITLE     Photoswitchable intein for light control of covalent protein binding
            and cleavage.
  JOURNAL   Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9.
  PUBMED    40935816
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-09-11"; volume: "16(1):8263. doi"; pages: " 
            10.1038/s41467-025-63595-9"
COMMENT     Sequence Label: Cp PS Intein dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..13219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             543..1478
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2130..2579
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     CDS             join(2667..2693,2694..2696,2697..5180,5181..5186,5187..5252,
                     5253..5255,5256..6770)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    6837..6861
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6891..7040
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7515..7871
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7890..8459
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     polyA_signal    9947..10068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9984..10003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10038..10057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10075..10530)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10212..10233)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10424..10443
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10557..10661
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10663..11020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10663..10854
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10686..10706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10871..11006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11055..11849
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11055..11849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11109..11128)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11719..11738
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12037..12056)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12081..12128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12457..13045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12946..12965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgaatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      541 tggctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta
      601 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg
      661 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga
      721 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga
      781 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg
      841 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc
      901 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg
      961 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg
     1021 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc
     1081 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga
     1141 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc
     1201 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca
     1261 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg
     1321 aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg
     1381 agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt
     1441 attacatcac gggggaggca gagggtttcc ctgccacagc tgggggtggt ggatcaggag
     1501 gtggtggatc aggaggaggt ggttctggtg gaggaggttc taccggtgaa atgatgctga
     1561 aaaaaatcct gaaaatcgag gaactggatg aacgcggtac cggcagcgga ggtcatacct
     1621 tgtatgcgcc ggggggttat gacatcatgg gttacctcat acagatcatg aataggccga
     1681 acccacaagt ggaactcgga cccgtcgata cctcctgcgc tctcattctg tgtgacctta
     1741 agcagaagga tacccctatc gtgtacgcct ccgaggcatt tctgtacatg acagggtact
     1801 cgaacgccga agtgctggga cggaactgcc gcttcctgca aagcccggat ggaatggtga
     1861 agcctaagtc aacccgcaaa tacgtggact ccaacactat caacaccatg cgcaaggcca
     1921 ttgaccgcaa tgctgaggtg caagtggaag tggtgaactt caagaagaat ggacagcgct
     1981 tcgtcaactt cctgactatg atccccgtgc gcgacgagac cggcgaatat cggtacagca
     2041 tggggtttca gtgtgagaca gagggcggct caggaggcag cggcggttct ggaggttccg
     2101 gtggcggctc aggaggtagc ggagggtctc acactcttta cgcccctgga ggatacgaca
     2161 ttatgggata tttgattcag attatgaacc gcccaaaccc tcaggtcgaa ctggggcctg
     2221 tggacacgtc atgtgccctg atcctgtgcg atctgaagca aaaggacact ccgattgtct
     2281 acgcctcgga agccttcttg tatatgaccg gatacagcaa tgcagaggtg ctcggtagga
     2341 actgcagatt cctgcagtcc cccgacggga tggtgaaacc aaagtccact cgcaaatatg
     2401 tggactcgaa cacgatcaat acaatgcgga aggccatcga ccggaacgcc gaggtccagg
     2461 tggaggtggt caactttaag aagaacggcc agcggttcgt gaactttctc accatgattc
     2521 cggtccggga tgaaaccgga gagtacagat actccatggg attccagtgc gaaaccgaag
     2581 ggtcaggatc cgaactgatt gatattgaag ttagcggtaa ccacctgttc tatgccaatg
     2641 atattctgac ccataatagc tcgagcatgg acaagaagta ctccattggg ctcgctatcg
     2701 gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat
     2761 tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc
     2821 tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat
     2881 atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta
     2941 aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa
     3001 agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt
     3061 acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc
     3121 ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg
     3181 gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt
     3241 acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc
     3301 tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg
     3361 agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact
     3421 ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa gacacctacg
     3481 atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac ctttttttgg
     3541 cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg aacacggaga
     3601 tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac caccaagact
     3661 tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag gaaattttct
     3721 tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc caggaggaat
     3781 tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag ctgctggtaa
     3841 agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga agcatccccc
     3901 accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat ttctacccct
     3961 ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata ccctactatg
     4021 taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa tcagaagaga
     4081 ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc cagtccttca
     4141 tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt cctaaacact
     4201 ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa tacgtcacag
     4261 aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc gtggacctcc
     4321 tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagactat ttcaaaaaga
     4381 ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac gcatccctgg
     4441 gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac aatgaggaga
     4501 acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat agggagatga
     4561 ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg aaacagctca
     4621 agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat gggatccgag
     4681 acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga
     4741 acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac
     4801 aagtttctgg ccagggggac agtcttcacg agcacatcgc taatcttgca ggtagcccag
     4861 ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg
     4921 gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga
     4981 agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg
     5041 ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct
     5101 acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc
     5161 ggctctccga ctacgacgtg gctgctatcg tgccccagtc ttttctcaaa gatgattcta
     5221 ttgataataa agtgttgaca agatccgata aagctagagg gaagagtgat aacgtcccct
     5281 cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga
     5341 tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg
     5401 ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg
     5461 cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag
     5521 aggtgaaagt tattactctg aagtctaagc tggtctcaga tttcagaaag gactttcagt
     5581 tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag
     5641 tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag
     5701 actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg
     5761 ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac
     5821 tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa
     5881 tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg
     5941 tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc
     6001 cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg
     6061 gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag
     6121 ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat
     6181 caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa
     6241 aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac
     6301 gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat
     6361 acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct cccgaagata
     6421 atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc
     6481 aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt
     6541 ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac attatccact
     6601 tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag
     6661 acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa
     6721 ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg
     6781 acggaggcgg tgggtctgga tcgccaggga tccgtcgact tgacgcgttg atatcaacaa
     6841 gtttgtacaa aaaagcaggc tacaaagagg ccagcggttc cggacgggct gacgcattgg
     6901 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
     6961 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
     7021 atttcgacct ggacatgctg attaactcta gaggttccgg atctggcggt gggggaagca
     7081 cgcgtggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga
     7141 agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg
     7201 accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa
     7261 aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt
     7321 tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc
     7381 ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac
     7441 tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc
     7501 caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc
     7561 tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt
     7621 tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc
     7681 ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc
     7741 tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc
     7801 tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgat atggatttct
     7861 cagccttgct gggctctggc agcggcagcc gggattccag ggaagggatg tttttgccga
     7921 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
     7981 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
     8041 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
     8101 cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
     8161 aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
     8221 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
     8281 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
     8341 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
     8401 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgttta
     8461 ctagtggagg gtcaggggct ggagggtcgg gggcaggcgg ttcgggagcc ggaggatctg
     8521 gagggggtgg ttcgggtggt ggtggtagcg gctactgcct ggacctgaag acccaggtgc
     8581 agacccccca gggcatgaag gagatcagca acatccaggt gggcgacctg gtgctgagca
     8641 acaccggcta caacgaggtg ctgaacgtgt tccccaagag caagaagaag agctacaaga
     8701 tcaccctgga ggacggcaag gagatcatct gcagcgagga gcacctgttc cccacccaga
     8761 ccggcgagat gaacatcagc ggcggcctga aggagggcat gtgcctgtac gtgaagggcg
     8821 gctccggagg aggtagtggc ggagggagtg gtgggagtgg ctctggttca ggcagtggtt
     8881 ctgcaggcga tatgcgggcc gctaatttgt ggccttctcc tttgatgatt aagagaagta
     8941 aaaaaaattc cttggctctg agcttgaccg ctgatcaaat ggtgtccgct ctcctcgacg
     9001 ccgaacctcc tatcttgtac agcgaatacg accccacacg gccatttagc gaggcctcaa
     9061 tgatgggact gcttacaaat ctcgctgata gagaactcgt gcatatgatt aattgggcca
     9121 aacgggtccc cggattcgtc gacctgactc tgcatgacca agtgcatctc ttggagtgcg
     9181 catggctgga aattctcatg atcggcctgg tgtggcggag catggaacat cctgtcaaac
     9241 tgctcttcgc ccccaatctg ctgctggatc ggaatcaagg caagtgcgtg gaaggaatgg
     9301 tcgaaatttt tgatatgctc ctcgccacca gcagcaggtt tagaatgatg aacctccaag
     9361 gcgaagagtt cgtctgtctg aagagcatca tcctgctgaa cagcggggtc tataccttcc
     9421 taagtagtac attaaaaagt ttggaggaaa aagatcacat tcatcgggtg ctcgataaaa
     9481 ttaccgatac cctaattcat ctcatggcta aagccggact cacactccaa caacaacatc
     9541 aaagactcgc tcaactgtta ctgattctga gccatattag acatatgagc aataagggaa
     9601 tggaacacct ctattccatg aaatgtaaaa atgtcgtccc tctgtacgat ctcctcctcg
     9661 aagccgccga tgctcatagg ctgcacgccc ctacctccag aggcggagcc agcgtcgaag
     9721 aaaccgatca gtcccatctg gctaccgccg gaagcacctc cagccacagc cttcagaaat
     9781 actatattac cggcgaagcc gaaggctttc ccgctaccgc ctaaagcggc cgcgactcta
     9841 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9901 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
    10081 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
    10141 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
    10201 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
    10261 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
    10321 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
    10381 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
    10441 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
    10501 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
    10561 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10621 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
    10681 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10741 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10801 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10921 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10981 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
    11041 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    11101 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    11161 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    11221 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    11281 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    11341 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    11401 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    11461 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    11521 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    11581 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    11641 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    11701 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    11761 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    11821 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    11881 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    11941 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    12001 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
    12061 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
    12121 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    12181 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    12241 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
    12301 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    12361 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    12481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    12541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    12601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    12661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    12721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    12781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    12841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    12901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    12961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    13021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    13081 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    13141 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
    13201 gcccatatat ggagttccg
//
