LOCUS       sequence-483153-        8253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human eIF3h, eIF3d and eIF3m subunits for further cloning or 
            expression in insect cells for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8253)
  TITLE     Ramakrishnan lab plasmids 2025
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBIG1B-eIF3hdm
FEATURES             Location/Qualifiers
     source          1..8253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(209..242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(288..307)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    332..380
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        475..566
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1835..1969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2125..2216
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3923..3949
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4109..4243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4399..4490
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5825..5959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  6273..6438
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6628..7216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7117..7136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  7351..7574
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7641..8174)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      181 ggcaaccttg ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt
      241 atctgggttt aaactatatc tcaatcgggg tttttatcga ggctaactga aacacggaag
      301 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      361 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcaa
      421 gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta atagatcatg
      481 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
      541 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
      601 gcggatccct cgagtcaaca tggcttccag aaaggagggt acaggatcta ctgctacttc
      661 cagctcttcc acagctggag ctgctggtaa aggtaaaggc aagggaggtt ctggtgacag
      721 cgctgttaag caggtccaaa tcgacggtct ggtggtgctg aagatcatca agcattacca
      781 ggaagagggt caaggtactg aagttgtgca gggtgtgctc ctgggtctgg tggtcgagga
      841 tagactggag atcaccaact gtttcccttt ccctcagcat accgaagatg atgccgactt
      901 cgatgaggtc cagtatcaaa tggagatgat gcgcagcctg aggcatgtca acatcgacca
      961 tctgcatgtg ggatggtatc aatccacata ctatggctcc tttgttacta gggctctcct
     1021 ggactctcag ttctcttacc aacacgctat tgaggaatct gtggtgctga tctacgatcc
     1081 catcaagact gctcagggat ctctctccct caaggcatat cgcctgactc ctaagctgat
     1141 ggaggtctgc aaagagaagg acttcagccc tgaagctctc aagaaggcaa acattacatt
     1201 cgagtacatg tttgaagagg tgcctatcgt tatcaagaac tcccacctca tcaacgtcct
     1261 gatgtgggag ctggagaaga agtctgctgt tgctgacaag catgaactgc tgagcctcgc
     1321 ttcctccaac catctcggaa agaacctgca gctcctcatg gacagggttg atgaaatgag
     1381 ccaagacatc gtgaagtaca acacctacat gaggaatacc tccaagcagc agcagcagaa
     1441 acatcaatac caacaaagaa gacaacagga gaacatgcaa cgccagagca gaggcgaacc
     1501 tccactccct gaagaggatc tgtccaagct gttcaagcca cctcagccac ctgctagaat
     1561 ggactctctg ctgatcgcag gccagatcaa tacctattgt cagaacatca aggagttcac
     1621 tgcccagaac ctcggtaagc tgttcatggc acaggcactc caagaataca acaattaagg
     1681 tacctctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag
     1741 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     1801 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1921 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
     1981 tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     2041 ccgctgagca ataactatca taaccattta aataaactgg atactattgc acgtttaaat
     2101 cgacctactc cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa
     2161 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc
     2221 cggattattc ataccgtccc accatcgggc gcggatccct cgagtcaaca tggctaagtt
     2281 catgactcca gtgatccaag acaacccttc tggttggggt ccttgtgctg tgcctgaaca
     2341 gtttagagac atgccctacc aaccattctc caagggtgat aggctcggta aagtcgcaga
     2401 ttggactgga gccacctacc aagacaagag atacaccaac aagtacagct cccagtttgg
     2461 aggaggaagc cagtacgctt actttcatga ggaggacgaa tcctctttcc agctcgtcga
     2521 tactgccagg acacagaaga ccgcttacca acgcaacagg atgaggtttg cccagcgcaa
     2581 tctcaggaga gacaaggaca ggaggaacat gctgcaattc aacctccaga tcctccctaa
     2641 gagcgctaaa cagaaagaga gagagaggat caggctgcag aagaagttcc agaaacagtt
     2701 tggagtgagg cagaaatggg atcagaagtc ccagaagcca agggattcct ccgttgaggt
     2761 ccgcagcgat tgggaggtta aggaagaaat ggacttccct cagctgatga agatgagata
     2821 cctggaagtg tccgaaccac aagacatcga gtgttgtggt gctctggagt actacgataa
     2881 ggccttcgac aggattacta ctcgctccga gaagccactg cgctccatca agagaatctt
     2941 ccacactgtg acaacaactg acgacccagt cattcgcaag ctggctaaga ctcaaggaaa
     3001 cgtcttcgct actgatgcta tcctggccac tctgatgagc tgcaccaggt ctgtctattc
     3061 ctgggacatt gtggtgcaga gagtgggatc taaactgttc ttcgataaga gggacaattc
     3121 cgatttcgat ctcctgaccg tctccgagac tgccaacgaa ccacctcaag atgaaggcaa
     3181 cagcttcaac agcccacgca atctggctat ggaggctact tacatcaacc acaacttctc
     3241 ccaacagtgt ctccgcatgg gtaaggagag gtacaacttt ccaaatccca atcctttcgt
     3301 tgaagacgat atggacaaga acgagatcgc ctccgttgct tatcgctata gacgctggaa
     3361 gctgggagac gacattgacc tgatcgttag gtgcgaacat gacggagtga tgacaggtgc
     3421 aaacggtgag gttagcttca tcaacatcaa gacactgaac gagtgggatt ctaggcactg
     3481 caatggtgtg gactggagac agaaactcga tagccagaga ggtgctgtta tcgctacaga
     3541 gctgaagaac aactcctaca aactggctcg ctggacttgt tgtgctctgc tcgctggaag
     3601 cgaatacctg aagctgggtt atgtttctcg ctaccatgtg aaggatagca gcagacatgt
     3661 gatcctcggc acccagcagt tcaagcctaa cgagttcgcc agccagatca acctcagcgt
     3721 cgagaacgct tggggtatcc tgagatgcgt tatcgacatc tgcatgaaac tcgaagaagg
     3781 caagtacctg atcctgaaag atccaaacaa acaggtgatt agggtgtact ctctcccaga
     3841 cggcaccttc tcttccgatg aggacgaaga ggaggaagaa gaagaggaag aagaggagga
     3901 ggaagaggag acaggctcct cctaccctta tgacgtccct gattacgcct aaggtacctc
     3961 tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac
     4021 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     4081 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     4141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4201 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     4261 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     4321 agcaataact atcataacca tttaaataaa ctggatacta ttgcacgttt aaatcgacct
     4381 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
     4441 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
     4501 attcataccg tcccaccatc gggcgcggat ccctcgagtc aacatgagcg tccctgcttt
     4561 catcgacatc tctgaagaag accaagcagc cgaactgagg gcttatctga agagcaaggg
     4621 agctgaaatc tctgaggaga acagcgaggg aggcctgcac gtcgatctcg ctcagatcat
     4681 cgaggcatgc gatgtgtgcc tgaaggagga cgacaaggat gtggaaagcg ttatgaatag
     4741 cgttgtgtcc ctcctcctga tcctcgaacc cgataagcaa gaagctctca tcgagtccct
     4801 gtgcgagaag ctcgtgaagt tcagggaggg agaaagacct agcctcaggc tccagctcct
     4861 ctccaacctg tttcatggaa tggacaagaa cactccagtc aggtacaccg tttactgttc
     4921 tctcatcaag gttgctgcaa gctgcggtgc tatccagtac attccaaccg aactggacca
     4981 agtccgcaaa tggatctctg actggaacct cactactgag aagaaacaca ccctgctgag
     5041 gctgctgtat gaagctctgg tggactgcaa gaagagcgac gctgcctcta aagtgatggt
     5101 ggagctgctg ggttcctaca ctgaggacaa tgcttcccag gccagggtcg atgctcatag
     5161 gtgcattgtt agagccctga aggacccaaa tgctttcctg ttcgaccacc tcctgaccct
     5221 caaacccgtg aagtttctgg aaggcgaact gatccatgat ctcctgacca tcttcgtgag
     5281 cgctaagctc gcctcttacg tcaagttcta ccagaacaac aaggacttca tcgactccct
     5341 gggactgctg catgaacaga acatggccaa gatgagactg ctgaccttca tgggaatggc
     5401 tgttgagaac aaggagatta gcttcgacac tatgcaacag gaactccaga tcggagcaga
     5461 tgatgtggag gctttcgtca tcgacgcagt tagaactaag atggtttact gcaagattga
     5521 tcagactcag cgcaaagtcg tcgtgtctca cagcactcac agaacattcg gtaagcagca
     5581 gtggcaacag ctgtatgaca ctctgaacgc ttggaagcag aacctcaaca aggtgaagaa
     5641 ctctctcctg agcctctctg acacctaagg tacctctaga gcctgcagtc tcgacaagct
     5701 tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5941 atcttatcat gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag
     6001 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca taaccattta
     6061 aataaatcta tatctcaatc ggggttagtc gataccccac cgagacccca ttgggaccaa
     6121 tacgcccgcg tttcttcctt ttccccaccc caacccccaa gttcgggtga aggcccaggg
     6181 ctcgcagcca acgtcggggc ggcaagccct gccatagcca ctacgggtta attaaaaact
     6241 ggatactatt gcacgtttaa acaggagatc cgaaccagat aagtgaaatc tagttccaaa
     6301 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     6361 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     6421 ctattttgtc cgcccacaac cggttgactt gggtcaactg tcagaccaag tttactcata
     6481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct
     7321 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     7381 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     7441 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     7501 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     7561 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     7621 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     7681 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     7741 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     7801 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     7861 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     7921 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     7981 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     8041 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     8101 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     8161 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     8221 ttgctgcttg gatgcccgag gcatagactg tac
//
