LOCUS       sequence-48317-e        6119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Liang FS, Ho WQ, Crabtree GR
  TITLE     Engineering the ABA plant stress pathway for regulation of induced 
            proximity.
  JOURNAL   Sci Signal. 2011 Mar 15;4(164):rs2.
  PUBMED    21406691
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2011-03-15"; volume: "4(164)"; pages: "rs2"
COMMENT     Sequence Label: SV-ABAactDA
FEATURES             Location/Qualifiers
     source          1..6119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2292..2312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2297..2626
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2477..2612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2539..2558
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2670..2690
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2691..2924
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3465..3491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3502..4075
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3525..4077
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3669..3686)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3896..3915
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4089..4529
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5430..5453
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5574..5695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5611..5630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5665..5684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5725..5742)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5725..5741)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5734..5756)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5950..5969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6069..6091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctg gccggcccgt acggccacgc ggatctcgac attgattatt
     2281 gactagtctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2401 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2461 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2521 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     2581 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     2641 ttgtatatcc attttcggaa ttcatgggcc ctaaaaagaa gcgtaaagtc gcccccccga
     2701 ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg
     2761 ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg
     2821 gatttacccc ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg
     2881 agcagatgtt taccgatgcc cttggaattg acgagtacgg tgggggcgcg ccaactcaag
     2941 acgaattcac ccaactctcc caatcaatcg ccgagttcca cacgtaccaa ctcggtaacg
     3001 gccgttgctc atctctccta gctcagcgaa tccacgcgcc gccggaaaca gtatggtccg
     3061 tggtgagacg tttcgatagg ccacagattt acaaacactt catcaaaagc tgtaacgtga
     3121 gtgaagattt cgagatgcga gtgggatgca cgcgcgacgt gaacgtgata agtggattac
     3181 cggcgaatac gtctcgagag agattagatc tgttggacga tgatcggaga gtgactgggt
     3241 ttagtataac cggtggtgaa cataggctga ggaattataa atcggttacg acggttcata
     3301 gatttgagaa agaagaagaa gaagaaagga tctggaccgt tgttttggaa tcttatgttg
     3361 ttgatgtacc ggaaggtaat tcggaggaag atacgagatt gtttgctgat acggttatta
     3421 gattgaatct tcagaaactt gcttcgatca ctgaagctat gaactaccca tacgatgttc
     3481 cagattacgc ttgaggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     3541 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3601 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3661 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3721 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3781 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3841 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3901 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3961 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4021 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4081 ccgagctcat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
     4141 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
     4201 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
     4261 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     4321 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     4381 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     4441 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     4501 acaaaggtca aagacagttg actgtatcga cgcgtgtgcc tttgtatggt tttacttcga
     4561 tttgtggaag aagacctgag atggaagctg ctgtttcgac tataccaaga ttccttcaat
     4621 cttcctctgg ttcgatgtta gatggtcggt ttgatcctca atccgccgct catttcttcg
     4681 gtgtttacga cggccatggc ggttctcagg tagcgaacta ttgtagagag aggatgcatt
     4741 tggctttggc ggaggagata gctaaggaga aaccgatgct ctgcgatggt gatacgtggc
     4801 tggagaagtg gaagaaagct cttttcaact cgttcctgag agttgactcg gagattgagt
     4861 cagttgcgcc ggagacggtt gggtcaacgt cggtggttgc cgttgttttc ccgtctcaca
     4921 tcttcgtcgc taactgcggt gactctagag ccgttctttg ccgcggcaaa actgcacttc
     4981 cattatccgt tgaccataaa ccggatagag aagatgaagc tgcgaggatt gaagccgcag
     5041 gagggaaagt gattcagtgg aatggagctc gtgttttcgg tgttctcgcc atgtcgagat
     5101 ccattggcga tagatacttg aaaccatcca tcattcctga tccggaagtg acggctgtga
     5161 agagagtaaa agaagatgat tgtctgattt tggcgagtga cggggtttgg gatgtaatga
     5221 cggatgaaga agcgtgtgag atggcaagga agcggattct cttgtggcac aagaaaaacg
     5281 cggtggctgg ggatgcatcg ttgctcgcgg atgagcggag aaaggaaggg aaagatcctg
     5341 cggcgatgtc cgcggctgag tatttgtcaa agctggcgat acagagagga agcaaagaca
     5401 acataagtgt ggtggtggtt gatttgaagg attacaagga cgatgacgat aagtgaagcg
     5461 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttacc
     5521 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5581 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5641 catttttttc actgcattgt agttgtggtt tgtccaaact catcaatgta tcttaagatc
     5701 tggcccttaa ggccggccaa attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5761 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5821 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5881 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     5941 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     6001 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6061 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
