LOCUS       sequence-48319-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  TITLE     David Wang unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDKIER
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccgag ctcggatccc ccgggctgca ggaattcatg
      121 gataaaactt tatctagaga agaggcaaag cagctaatgc aacttttatg tttggacatg
      181 agttgctggg gcaatttacc actaatgaga agacaatact tagtaaagtg caaagaatat
      241 catccagata aaggaggaaa tgaggaaagc atgaagcttc ttaattcttt atatttaaaa
      301 ctgcaagact cagttagcag tgtacatgat ttaaatgaag aagaagataa tatatggcaa
      361 tcttctcagg tatactgcaa agatttgtgc tgcaataagt ttagattagt tggtgctata
      421 tatggtgaat actatgaagc atatattatg aagcagtggg atgtatgcat tcatggctac
      481 aatcacgaat gccaatgcat tcactgtatt ttaagcaaat atcataaaga aaagtacaaa
      541 atttatagaa aaccacctgt gtggatagag tgctactgct ataagtgcta cagggagtgg
      601 tttttttttc caatttcaat gcaaacattt ttcttttgga aagtgattat ttttaatact
      661 gaaatacgag cagttcaacc attattacgg taagcataaa ataatgtttt taaataattg
      721 tttgtgtaat attaaatgta tatttttgtt gtatagatac ctacatacgg tacacctgac
      781 tgggatgagt ggtggtcaca gttca
//
