LOCUS       sequence-48323-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     Rimm Lab Lenti MetGFP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-tkd2MetGFP
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(866..887)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagaggatc caccatgcta ccatacatga aacatggaga
      121 tcttcgaaat ttcattcgaa atgagactca taatccaact gtaaaagatc ttattggctt
      181 tggtcttcaa gtagccaaag gcatgaaata tcttgcaagc aaaaagtttg tccacagaga
      241 cttggctgca agaaactgta tgctggatga aaaattcaca gtcaaggttg ctgattttgg
      301 tcttgccaga gacatgtatg ataaagaata ctatagtgta cacaacaaaa caggtgcaaa
      361 gctgccagtg aagtggatgg ctttggaaag tctgcaaact caaaagttta ccaccaagtc
      421 agatgtgtgg tcctttggcg tgctcctctg ggagctgatg acaagaggag ccccacctta
      481 tcctgacgta aacacctttg atataactgt ttacttgttg caagggagaa gactcctaca
      541 acccgaatac tgcccagacc ccttatatga agtaatgcta aaatgctggc accctaaagc
      601 cgaaatgcgc ccatcctttt ctgaactggt gtcccggata tcagcgatct tctctacttt
      661 cattggggag cactatgtcc atgtgaacgc tacttatgtg aacgtaaaat gtgtcgctcc
      721 gtatccttct ctgttgtcat cagaagataa cgctgatgat gaggtggaca cacgaccagc
      781 ctccttctgg gagacatcag cggatccacc ggtcgccacc atggtgagca agggcgagga
      841 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      901 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      961 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1021 cgg
//
