LOCUS       sequence-48324-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     Rimm Lab Lenti MetGFP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-tkd2MetGFP
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..153
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     104..124
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     150..174
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      959..968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
       61 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      121 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      181 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag gatccaccat
      241 gctaccatac atgaaacatg gagatcttcg aaatttcatt cgaaatgaga ctcataatcc
      301 aactgtaaaa gatcttattg gctttggtct tcaagtagcc aaaggcatga aatatcttgc
      361 aagcaaaaag tttgtccaca gagacttggc tgcaagaaac tgtatgctgg atgaaaaatt
      421 cacagtcaag gttgctgatt ttggtcttgc cagagacatg tatgataaag aatactatag
      481 tgtacacaac aaaacaggtg caaagctgcc agtgaagtgg atggctttgg aaagtctgca
      541 aactcaaaag tttaccacca agtcagatgt gtggtccttt ggcgtgctcc tctgggagct
      601 gatgacaaga ggagccccac cttatcctga cgtaaacacc tttgatataa ctgtttactt
      661 gttgcaaggg agaagactcc tacaacccga atactgccca gaccccttat atgaagtaat
      721 gctaaaatgc tggcacccta aagccgaaat gcgcccatcc ttttctgaac tggtgtcccg
      781 gatatcagcg atcttctcta ctttcattgg ggagcactat gtccatgtga acgctactta
      841 tgtgaacgta aaatgtgtcg ctccgtatcc ttctctgttg tcatcagaag ataacgctga
      901 tgatgaggtg gacacacgac cagcctcctt ctgggagaca tcagcggatc caccggtcgc
      961 caccatggtg agcaagggcg ag
//
