LOCUS       sequence-48326-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN
  TITLE     Metabolic engineering of cofactor F420 production in Mycobacterium 
            smegmatis.
  JOURNAL   PLoS One. 2010 Dec 29;5(12):e15803.
  PUBMED    21209917
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-29"; volume: "5(12)"; pages: "e15803"
COMMENT     Sequence Label: pYUB28b
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             177..194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(247..265)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      261..308
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      345..773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(432..451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     642..663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact ggtggacagc
      121 aaatgggtcg ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
      181 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      241 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      301 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      781 aatatctaga tttaaagatc tggtaccgcg gccgcgccac ggatgccacc acaggcacta
      841 caacggagtt cgccacgtac atcaccacaa ccaccgattc tggcggtgag ctcc
//
