LOCUS       sequence-48327-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN
  TITLE     Metabolic engineering of cofactor F420 production in Mycobacterium 
            smegmatis.
  JOURNAL   PLoS One. 2010 Dec 29;5(12):e15803.
  PUBMED    21209917
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-29"; volume: "5(12)"; pages: "e15803"
COMMENT     Sequence Label: pYUBDuet
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    159..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(316..333)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             352..374
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    389..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(414..432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt
      121 atatctcctt cttatactta actaatatac taagatgggg aattgttatc cgctcacaat
      181 tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      241 tgttcgactt aagcattatg cggccgcaag cttgtcgacc tgcaggcgcg ccgagctcga
      301 attcggatcc tggctgtggt gatgatggtg atggctgctg cccatggtat atctccttct
      361 taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
      421 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
      481 cggcatcacc ggcgccgggg gcggtgtccg gcggccccca gaggaactgc gccagttcct
      541 ccggatcggt gaagccggag agatccagcg gggtctcctc gaacacctcg aagtcgtgca
      601 ggaaggtgaa ggcgagcagt tcgcgggcga agtcctcggt ccgcttccac tgcgccccgt
      661 cgagcagcgc ggccaggatc tcgcggtcgc cccggaaggc gttgagatgc agttgcacca
      721 ggctgtagcg ggagtctccc gcatagacgt cggtgaagtc gacgatcccg gtgacctcgg
      781 tcgcggccag gtccacgaag atgttggtcc cgtgcaggtc gccgtggacg aaccggggtt
      841 cgcggcc
//
