LOCUS       sequence-483330-        9100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mMaroon1 reporter plasmid for live cell visualization of RNA m6A 
            modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Li Q, Liu J, Guo L, Zhang Y, Chen Y, Liu H, Cheng H, Deng L, Qiu J, 
            Zhang K, Goh WSS, Wang Y, Peng Q
  TITLE     Decoding the interplay between m(6)A modification and stress granule
            stability by live-cell imaging.
  JOURNAL   Sci Adv. 2024 Nov 15;10(46):eadp5689. doi: 10.1126/sciadv.adp5689. 
            Epub 2024 Nov 15.
  PUBMED    39546601
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-11-15"; volume: "10(46):eadp5689. doi"; pages: " 
            10.1126/sciadv.adp5689. Epub 2024 Nov 15"
COMMENT     Sequence Label: pLD1112 TRE-NLS-mMaroon1-NLS-22Xm6A MS2
FEATURES             Location/Qualifiers
     source          1..9100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             552..578
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1290..1316
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_RNA        1353..1371
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1381..1399
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1409..1427
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1437..1455
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1465..1483
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1493..1511
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1521..1539
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1549..1567
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1577..1595
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1605..1623
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1633..1651
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1661..1679
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1689..1707
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1717..1735
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1745..1763
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1773..1791
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1801..1819
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1829..1847
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1857..1875
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1885..1903
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1913..1931
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1941..1959
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1969..1987
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        1997..2015
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        2042..2552
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2471..2489
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2562..3158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2562..2581)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3058..3078
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3159..3212
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3213..3830,3831..3836,3837..3872,3873..3875,3876..3911,
                     3912..3914,3915..3950,3951..3959)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3999..4019)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4417..4428)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4633..4866
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4938..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4975..4994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5029..5048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5099..5234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5234..5305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5306..5374,5375..6166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5524..5543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6337..6925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6826..6845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7079..7096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7213..7234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7249..7254,7255..7272,7273..7279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7287..7303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7292..7314
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7311..7327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7311..7327
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7346..7366
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7348..7366
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7394..7620
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7621..7801
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7848..7973
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8466..8699
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8884..8928
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gcccgcagca aagcgtgtaa agctagacat ggtgagcaag ggcgaggagc
      601 tgatcaagga gaacatgcac accaagctgt acctgacagg caccgtgaac aaccactact
      661 tcgaatgcac cgccgaaggg gagggcaagc cctacgaggg cacccagacc aacaggatca
      721 aggtggtgcg cggaggcccc ctgccgttcg cattcgacat cctggccccc tgctttatgt
      781 acgggagcaa gaccttcatt aaccatccac ctgacattcc cgattatttt aagcagtcct
      841 tccctgaggg cttcacatgg gagagaacca ccgtttacga agacgggggc acactcactg
      901 ctacacagga caccagcctc caggacggct gcctgatcta caacgtccaa gtcagagggg
      961 agaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccagta
     1021 ccgagaccct gtaccccgct gacggcagcc tggaaggcag actggactgg gccctgaagc
     1081 tcgtgggcgg gggccacctg cactgcagac tggaaaccac atacagatcc aagaaacccg
     1141 caaagaacct caagatgccc ggcgtctatt ttattgacag gagactggaa agaatcaagg
     1201 aggccgacaa tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     1261 tccctagcaa actggggcac aaacttaatc ccgcagcaaa gcgtgtaaag ctagactaac
     1321 tggtaccatg caattcgaag cttccggact gtacatgagg atcacccatg tccggactgt
     1381 acatgaggat cacccatgtc cggactgtac atgaggatca cccatgtccg gactgtacat
     1441 gaggatcacc catgtccgga ctgtacatga ggatcaccca tgtccggact gtacatgagg
     1501 atcacccatg tccggactgt acatgaggat cacccatgtc cggactgtac atgaggatca
     1561 cccatgtccg gactgtacat gaggatcacc catgtccgga ctgtacatga ggatcaccca
     1621 tgtccggact gtacatgagg atcacccatg tccggactgt acatgaggat cacccatgtc
     1681 cggactgtac atgaggatca cccatgtccg gactgtacat gaggatcacc catgtccgga
     1741 ctgtacatga ggatcaccca tgtccggact gtacatgagg atcacccatg tccggactgt
     1801 acatgaggat cacccatgtc cggactgtac atgaggatca cccatgtccg gactgtacat
     1861 gaggatcacc catgtccgga ctgtacatga ggatcaccca tgtccggact gtacatgagg
     1921 atcacccatg tccggactgt acatgaggat cacccatgtc cggactgtac atgaggatca
     1981 cccatgtccg gactgtacat gaggatcacc catgtgcggc cgcgatcgtg aattcggatc
     2041 cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     2101 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     2161 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     2221 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     2281 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     2341 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     2401 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     2461 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     2521 tcgttgaccg aatcaccgac ctctctcccc agcaattcac catgaccgag tacaagccca
     2581 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     2641 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     2701 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     2761 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2821 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     2881 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     2941 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     3001 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3061 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     3121 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccga aggtagaggt tctctcctca
     3181 cttgtggtga tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca
     3241 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     3301 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     3361 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     3421 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     3481 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     3541 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     3601 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     3661 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     3721 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     3781 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     3841 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     3901 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     3961 taagtaagga tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt
     4021 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4081 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4141 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4201 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4261 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4321 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4381 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4441 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4501 acgagtcgga tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag
     4561 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4621 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4681 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4741 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4801 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4861 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5221 cttttttgga ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5281 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5341 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5401 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5461 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5521 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5581 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5641 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5701 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5761 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5821 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5881 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5941 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6001 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6061 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6121 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6181 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6241 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6301 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6421 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6481 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6541 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6601 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6661 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6721 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6781 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6841 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6901 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6961 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7021 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7081 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7201 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7261 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7321 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     7381 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     7441 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     7501 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     7561 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     7621 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7681 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7741 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7801 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7861 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7921 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7981 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     8041 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     8101 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     8161 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     8221 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     8281 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     8341 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8401 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8461 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8521 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8581 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8641 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8701 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8761 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8821 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8881 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8941 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9001 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9061 ccattatcgt ttcagaccca cctcccaacc ccgaggggac
//
