LOCUS       sequence-48335-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pIC389
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             103..147
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctcggcatgg acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc
       61 cgctctagag agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct
      121 aaattcgaac gccagcacat ggacagcgga ggtggaggta ctagtctcga gcgacttaat
      181 ctctcagacc ttcaaatggt tcttggtctg agctacagca caaataactc cataatgtct
      241 gaaaatactg aaattgatag cagtttgaat ggtgtacgcc ccaagttggg acgccggcat
      301 agtatggaaa atatggaact tatgaagtta acaccagaaa aggtacagaa ctggaacagt
      361 gaaattcttg ctaagcaaaa acctcttatt gccaaacctt ctgcaaagct actctttgtc
      421 aacagactga aggggaaaaa atacaaaacg aattcctcca ctaaagttct ccaagatgcc
      481 agtaattcta ttgaccaccg aattccaaat tctcagagga aaatcagtgc agatactatt
      541 ggagatgaag ggttctttga cttattaagc cgatttcaaa gcaataggat ggatgatcag
      601 agatgttgct tacaagaaaa gaactgccat acagcttcaa caacaacttc cactccccct
      661 aaaatgatgc taaaaacatc atctgttcct gtggtatccc ccaacacgga tgagttttta
      721 gatcttcttg ccagctcaca gagtcgccgt ctggatgacc agagggctag tttcagtaat
      781 ttgcca
//
