LOCUS       sequence-48336-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pCB163
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             102..146
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tcggcatgga cgagctgtac aagtccggag gggatcggtg gagctccacc ggtggcggcc
       61 gctctagaga gaatctttat tttcagggcg ccgccaaatt caaagaaacc gctgctgcta
      121 aattcgaacg ccagcacatg gacagcggag gtggaggtac tagtctcgag atggcggacc
      181 ctaaataccc cgaccttccc ggcattgcca ggaatgagcc agatgtttat gaaactagcg
      241 acctacctga ggatgatcaa gcagagttcg atgcggagga gctgacaagc acaagtgtgg
      301 aacacatcat tgtcaatcct aatgctgcct atgacaagtt caaggacaag agagtgggga
      361 caaagggact tgatttctca gatcgtattg gaaaaaccaa gaggacagga tatgaatctg
      421 gagaatatga gatgcttgga gagggtctgg gagtgaagga gacaccccag caaaagtacc
      481 agcgcctact gcatgaggtc caagagctga caactgaagt tgaaaaaatc aagacgacag
      541 tgaaggagtc agccacagag gagaagctga cccctgtgtt gctggctaaa cagctggcag
      601 ccctgaagca gcagctggtt gcttcccacc tggagaagct gctgggacca gatgctgcaa
      661 tcaaccttac cgaccccgat ggcgccctgg ctaagcgcct actactgcag ctggaagcaa
      721 caaagaacag caaaggggga tca
//
